Functional exosome-mimic for delivery of siRNA to cancer: in vitro and in vivo evaluation

Abstract

Exosomes, the smallest subgroup of extracellular vesicles, have been recognized as extracellular organelles that contain genetic and proteomic information for long distance intercellular communication. Exosome-based drug delivery is currently a subject of intensive research. Here, we report a novel strategy to produce nanoscale exosome-mimics (EMs) in sufficient quantity for gene delivery in cancer both in vitro and in vivo. Size-controllable EMs were generated at a high yield by serial extrusion of non-tumorigenic epithelial MCF-10A cells through filters with different pore sizes. siRNA was then encapsulated into the EMs by electroporation. Biosafety and uptake efficiency of the EMs were evaluated both in vitro and in vivo. The mechanism underlying their cellular endocytosis was also studied.

Evaluation of antitumor activity of survivin short interfering RNA delivered by lipid nanoparticles in colon cancer in vitro and in vivo

“…Paduano et al (23) revealed that si-survivins markedly reduced the expression level of survivin and produced supra-additive growth suppression in human androgen-independent prostate cancer cells. Numerous previous studies have directly added siRNA mimics into cell cultures (35–37). However, the major limitations of direct addition of siRNA mimics to cells are the instability and short half-life time.…”

The Synergistic Effect of Microwave Radiation and Hypergravity on Rats and the Intervention Effect of Rana Sylvatica Le Conte Oil

“…The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST), creatine kinase (CK), isocitric dehydrogenase (ICDH), and hydroxybutyrate dehydrogenase (HBDH) in rat serum were determined by detection kits according to the instructions that were provided by Changzheng Biochemical Reagent Co, Ltd, Shanghai, China. 17 …”

Engineering Extracellular Vesicles as Nanotherapeutics for Regenerative Medicine

“…Though no control comparison was made to native EVs derived from the same β-cell line, a yield of 8.2 × 10 8 particles per 1 µg of total protein was reported, confirming that generally a high number of vesicles can be made from this method [105]. A similar protocol was used to produce EV mimics from human breast epithelial cells [106]. A significantly higher yield of mimics was seen, with 379.3 µg of protein and 3.0 × 10 11 of mimics produced in comparison to 2.5 µg of protein and 2.6 × 10 9 of EVs from the same number of cells (1.0 × 10 7 cells).…”