Functional evaluation of iodoacetic acid induced photoreceptor degeneration in the cat

Ye, Nan; Zhang, Qin; Ren, Chaoran; Huang, Xin; Gao, Jie; Li, Xiaoxin; Pu, Mingliang

doi:10.1007/s11427-013-4483-3

Cited by 7 publications

(11 citation statements)

References 22 publications

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“…3,7 Different behavioral tests, which were based on the ability to avoid obstacles, were used to assess vision in the IAA cat model and in the transgenic pig model of photoreceptor degeneration. 11,17 In both cases, animals were not trained, and the efficacy of the test varied between animals regardless of their vision status. In our current study, the OC test was designed to include a 2-wk training period to accustom the animals to the trial environment, including new smells and noises.…”

Section: Discussionmentioning

confidence: 99%

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Behavioral Assessment of Vision in Pigs

Barone

Nannoni

Elmi

et al. 2018

j am assoc lab anim sci

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Swine (Sus scrofa) are often the 'gold standard' laboratory animal for ophthalmology research due to the anatomic and physiologic similarities between the porcine and human eye and retina. Despite the importance of this model, few tools for behavioral vision assessment in pigs are available. The aim of this study was to identify and validate a feasible and reproducible behavioral test to assess vision in a pig model of photoreceptor degeneration. In addition, a robust behavioral test will reduce stress and enhance enrichment by allowing animals opportunities for environmental exploration and by reducing the number of invasive experimental procedures. Two distinct behavioral approaches were tested: the obstacle-course test and temperament test. In the obstacle-course test, pigs were challenged (after an initial training period) to navigate a 10-object obstacle course; time and the number of collisions with the objects were recorded. In the temperament test, the time needed for pigs to complete 3 different tasks (human-approach, novel-object, and open-door tests) was recorded. The obstacle-course test revealed significant differences in time and number of collisions between swine with vision impairment and control animals, and the training period proved to be pivotal to avoid bias due to individual animal characteristics. In contrast, the temperament test was not altered by vision impairment but was validated to measure stress and behavioral alterations in laboratory pigs undergoing experimental procedures, thus achieving marked refinement of the study.

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Section: Discussionmentioning

confidence: 99%

“…OC test. Nine pigs treated with IAA and 5 control animals were evaluated by using a previously described behavioral test 17 with specific modifications. The pigs enrolled in the test were divided into 3 groups (Table 1), to facilitate their management.…”

Section: Behavioral Assessment Of Vision In Pigsmentioning

confidence: 99%

Behavioral Assessment of Vision in Pigs

Barone

Nannoni

Elmi

et al. 2018

j am assoc lab anim sci

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“…Another cat model, the CrxRdy cats, develops retinal thinning that initially takes place in the central retina [ 159 ]. An acute, reliable, and complete photoreceptor degeneration model in cats can be achieved by ear vein injection of high-dose iodoacetic acid [ 161 ]. Retinal sheet transplantation studies conducted in Abyssinian cats showed good signs of transplant integration with the host retina and lamination of transplant photoreceptors.…”

Section: Animal Models For Stem Cell-based Therapiesmentioning

confidence: 99%

Assessment of Safety and Functional Efficacy of Stem Cell-Based Therapeutic Approaches Using Retinal Degenerative Animal Models

Lin

Seiler

Zhu

et al. 2017

Stem Cells International

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Dysfunction and death of retinal pigment epithelium (RPE) and or photoreceptors can lead to irreversible vision loss. The eye represents an ideal microenvironment for stem cell-based therapy. It is considered an "immune privileged" site, and the number of cells needed for therapy is relatively low for the area of focused vision (macula). Further, surgical placement of stem cell-derived grafts (RPE, retinal progenitors, and photoreceptor precursors) into the vitreous cavity or subretinal space has been well established. For preclinical tests, assessments of stem cell-derived graft survival and functionality are conducted in animal models by various noninvasive approaches and imaging modalities. In vivo experiments conducted in animal models based on replacing photoreceptors and/or RPE cells have shown survival and functionality of the transplanted cells, rescue of the host retina, and improvement of visual function. Based on the positive results obtained from these animal experiments, human clinical trials are being initiated. Despite such progress in stem cell research, ethical, regulatory, safety, and technical difficulties still remain a challenge for the transformation of this technique into a standard clinical approach. In this review, the current status of preclinical safety and efficacy studies for retinal cell replacement therapies conducted in animal models will be discussed.

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“…Among animal models, the eyes of felines and swine have a central region (comprised partly of photoreceptors) comparable to the human macula, with similar distributions of rod and cone cells in the central and peripheral retina [9]. Therefore, using iodoacetic acid (IAA), such animals have been designated as models of retinal degeneration [101112131415]. IAA reacts with the sulfhydryl groups of glyceraldehyde 3-phosphate dehydrogenase (GADPH) in cells of various organs, including the eye, and inhibits anaerobic glycolysis [161718192021].…”

mentioning

confidence: 99%

“…In this process, the inner retina is preserved and shows histological features that appear early in human RP [22]. Based on these characteristic findings of retinal degeneration, an animal model of IAA-induced retinal degeneration has been designed to explore effective treatment of RP disease in human eyes [101214].…”

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confidence: 99%

Analysis of Changes in Retinal Photoreceptors Using Optical Coherence Tomography in a Feline Model of Iodoacetic Acid-induced Retinal Degeneration

Noh

Lim²,

Lee

et al. 2019

Korean J Ophthalmol

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PurposeWe investigated structural changes in the retina by using optical coherence tomography (OCT) in a feline model of retinal degeneration using iodoacetic acid (IAA).MethodsWe examined 22 eyes of 11 felines over 2 years of age. The felines had fasted for 12 hours and were intravenously injected with IAA 20 mg/kg of body weight. OCT (Spectralis OCT) was performed at the point where the ends of the retinal vessels collected in the lateral direction from the optic nerve head and area centralis. Similarly, OCT was performed four times at 1-week intervals following injections, at which point the felines were sacrificed and histologic examinations were performed. Using OCT, the thickness of each layer of the retina was measured.ResultsThe average body weight of the three male and eight female felines investigated in this study was 1.61 ± 0.19 kg. The mean total retinal thickness of the felines before injection was 221.32 ± 9.82 µm, with a significant decrease in the retinal thickness at 2, 3, and 4 weeks following injections of 186.41 ± 35.42, 174.56 ± 31.94, and 175.35 ± 33.84 µm, respectively (p = 0.028, 0.027, and 0.027, respectively). The thickness of the outer nuclear layer was 57.49 ± 8.03 µm before injection and 29.26 ± 17.87, 25.62 ± 13.88, and 31.60 ± 18.38 µm at 2, 3, and 4 weeks, respectively, after injection (p = 0.028, 0.028, 0.046, respectively).ConclusionsIn a feline model of retinal degeneration using IAA, the total retinal thickness and the thickness of the outer nuclear layer were shown to decrease significantly on OCT.

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Functional evaluation of iodoacetic acid induced photoreceptor degeneration in the cat

Cited by 7 publications

References 22 publications

Behavioral Assessment of Vision in Pigs

Behavioral Assessment of Vision in Pigs

Assessment of Safety and Functional Efficacy of Stem Cell-Based Therapeutic Approaches Using Retinal Degenerative Animal Models

Analysis of Changes in Retinal Photoreceptors Using Optical Coherence Tomography in a Feline Model of Iodoacetic Acid-induced Retinal Degeneration

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