Functional Equivalency of B7-1 and B7-2 for Costimulating Plasmid DNA Vaccine-Elicited CTL Responses

Santra, Sampa; Barouch, Dan H.; Jackson, Shawn S.; Kuroda, Marcelo J.; Schmitz, Jörn E.; Lifton, Michelle A.; Sharpe, Arlene H.; Letvin, Norman L.

doi:10.4049/jimmunol.165.12.6791

Cited by 28 publications

(19 citation statements)

References 31 publications

(35 reference statements)

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“…Antibody (IgG), as detected by ELISA, was not produced upon plasmid or MVA immunization in any of the immunization groups, which is in agreement with previous VMV plasmid immunization studies in sheep [14] and [16], and with work in mice on immunization with HIV plasmids in the presence of CD80 or CD86 genes [49]. Just after challenge (week 16) and until the end of the experimental period, groups receiving B7 genes produced both anti--ENV and anti--GAG antibodies, as revealed by WB, whereas the gag-env group had anti--ENV but a very low production of anti--GAG antibodies at weeks 16 and 20 and the control group did not show either anti--ENV or anti--GAG antibodies until week 20.…”

Section: Discussionsupporting

confidence: 76%

“…The enhancement of CTL responses when incorporating CD86 into the vaccine is in agreement with a series of previous HIV studies in mice [46], [47] and [48] where inclusion of CD80 in the inoculum did not generate CTL responses. However, the relative role of both CD80 and CD86 molecules remains controversial, as there are studies in mice which either propose the enhancement of both cellular and humoral responses when using CD86 [49] or describe the enhancement of CTL responses when incorporating CD80 rather than CD86 [50].…”

Section: Discussionmentioning

confidence: 99%

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Use of B7 costimulatory molecules as adjuvants in a prime-boost vaccination against Visna/Maedi ovine lentivirus

Andrés

Reina

Ciriza

et al. 2009

Vaccine

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RNA transcripts of the B7 family molecule (CD80) are diminished in blood leukocytes from animals clinically affected with Visna/Maedi virus (VMV) infection. This work investigates whether the use of B7 genes enhances immune responses and protection in immunization--challenge approaches. Sheep were primed by particle--mediated epidermal bombardment with VMV gag and env gene recombinant plasmids together with plasmids encoding both CD80 and CD86 or CD80 alone, boosted with gag and env gene recombinant modified vaccinia Ankara virus and challenged intratracheally with VMV. Immunization in the presence of one or both of the B7 genes resulted in CD4+ T cell activation and antibody production (before and after challenge, respectively), but only immunization with CD80 and CD86 genes together, and not CD80 alone, resulted in a reduced number of infected animals and increased early transient cytotoxic T lymphocytes (CTL) responses. Post--mortem analysis showed an immune activation of lymphoid tissue in challenge--target organs in those animals that had received B7 genes compared to unvaccinated animals. Thus, the inclusion of B7 genes helped to enhance early cellular responses and protection (diminished proportion of infected animals) against VMV infection.

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Section: Discussionsupporting

confidence: 76%

Section: Discussionmentioning

confidence: 99%

Use of B7 costimulatory molecules as adjuvants in a prime-boost vaccination against Visna/Maedi ovine lentivirus

Andrés

Reina

Ciriza

et al. 2009

Vaccine

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“…Glycoprotein 120 by itself is weakly immunogenic and immunizations with plasmid DNA encoding gp120 alone are not efficient. DNA immunizations with gp120 of HIV usually require boosting with viral vaccines, co-administration of various cytokines or expression as a fusion protein with proinflammatory chemoattractants to achieve optimal efficacy [13][14][15][16][17].…”

Section: Introductionmentioning

confidence: 99%

Induction of gp120-specific protective immune responses by genetic vaccination with linear polyethylenimine–plasmid complex

Garzón

Berraondo

Crettaz

et al. 2005

Vaccine

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The induction of IFN-␥-secreting CD8+ T cells and neutralizing antibodies to HIV-1 are both key requirements for prevention of viral transmission and clearance of pathogenic HIV. Although DNA vaccination has been shown to induce both humoral and cellular immune responses against HIV antigens, the magnitude of the immune responses has always been disappointing. In this report, we analyze the ability of polyethylenimine (PEI)-DNA complex expressing an HIV-glycoprotein 120 (gp120) antigen (PEI-pgp120) to induce systemic CD8+ T cell and humoral responses to the gp120 antigen. The administration of PEI-plasmid complex resulted in rapid elevation of serum levels of IL-12 and IFN-␥. Furthermore, a single administration of PEI-pgp120 complex elicits a number of gp120-specific CD8+ T cells 20 times higher than that elicited by three intramuscular injections of naked DNA. Interestingly, we found that systemic vaccination with PEI-pgp120 induced protective immune responses against both systemic and mucosal challenges with a recombinant vaccinia virus expressing a gp120 antigen. The data also demonstrated that the depletion of macrophages with liposome-encapsulated clodronate completely abolished gp120-specific cellular response. Overall, our results showed that a single administration of PEI-pgp120 complexes, eliciting strong immune responses, is an effective vaccination approach to generate protection against systemic and mucosal viral infections.

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“…Tetrameric H-2D d complexes folded around the HIV-1 IIIB V3 loop optimal P18 epitope peptide (P18-I10 or RGPGRAFVTI) (36) were prepared and used to stain P18-specific CD8 ϩ T cells essentially as previously described (37,38). Mouse blood was collected in RPMI 1640 containing 40 U/ml heparin.…”

Section: Tetramer Staining Assaysmentioning

confidence: 99%

Potent CD4+ T Cell Responses Elicited by a Bicistronic HIV-1 DNA Vaccine Expressing gp120 and GM-CSF

Barouch

Santra

Tenner-Rácz

et al. 2002

The Journal of Immunology

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143

140

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Virus-specific CD4+ T cell responses have been shown to play a critical role in controlling HIV-1 replication. Candidate HIV-1 vaccines should therefore elicit potent CD4+ as well as CD8+ T cell responses. In this report we investigate the ability of plasmid GM-CSF to augment CD4+ T cell responses elicited by an HIV-1 gp120 DNA vaccine in mice. Coadministration of a plasmid expressing GM-CSF with the gp120 DNA vaccine led to only a marginal increase in gp120-specific splenocyte CD4+ T cell responses. However, immunization with a bicistronic plasmid that coexpressed gp120 and GM-CSF under control of a single promoter led to a dramatic augmentation of vaccine-elicited CD4+ T cell responses, as measured by both cellular proliferation and ELISPOT assays. This augmentation of CD4+ T cell responses was selective, since vaccine-elicited Ab and CD8+ T cell responses were not significantly changed by the addition of GM-CSF. A 100-fold lower dose of the gp120/GM-CSF bicistronic DNA vaccine was required to elicit detectable gp120-specific splenocyte proliferative responses compared with the monocistronic gp120 DNA vaccine. Consistent with these findings, i.m. injection of the gp120/GM-CSF bicistronic DNA vaccine evoked a more extensive cellular infiltrate at the site of inoculation than the monocistronic gp120 DNA vaccine. These results demonstrate that bicistronic DNA vaccines containing GM-CSF elicit remarkably potent CD4+ T cell responses and suggest that optimal Th cell priming requires the precise temporal and spatial codelivery of Ag and GM-CSF.

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Functional Equivalency of B7-1 and B7-2 for Costimulating Plasmid DNA Vaccine-Elicited CTL Responses

Cited by 28 publications

References 31 publications

Use of B7 costimulatory molecules as adjuvants in a prime-boost vaccination against Visna/Maedi ovine lentivirus

Use of B7 costimulatory molecules as adjuvants in a prime-boost vaccination against Visna/Maedi ovine lentivirus

Induction of gp120-specific protective immune responses by genetic vaccination with linear polyethylenimine–plasmid complex

Potent CD4+ T Cell Responses Elicited by a Bicistronic HIV-1 DNA Vaccine Expressing gp120 and GM-CSF

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