Functional ectopic neural lobe increases GAP-43 expression via PI3K/AKT pathways to alleviate central diabetes insipidus after pituitary stalk lesion in rats

Feng, Zhanpeng; Ou, Yichao; Zhou, Mingfeng; Wu, Guangsen; Ma, Linzi; Yuan, Zuyi; Liu, Yawei; Qi, Songtao

doi:10.1016/j.neulet.2018.02.038

Cited by 13 publications

(11 citation statements)

References 22 publications

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“…All animals were housed in independent metabolic cages for 3 days to accommodate environment before surgery. PEL surgery was performed according to our previous studies . In brief, rats were first anesthetized with 5% isoflurane with the gas mask fitted into the platform of the stereotaxic instrument (Stoelting).…”

Section: Methodsmentioning

confidence: 99%

“…PEL surgery was performed according to our previous studies. 7,8 In brief, rats were first anesthetized with 5% isoflurane with the gas mask fitted into the platform of the stereotaxic instrument (Stoelting). Anesthesia was maintained with 1.5%-2% isoflurane delivered in air at 0.5 L/ min during the whole surgery process.…”

Section: Pituitary Stalk Electric Lesion Surgerymentioning

confidence: 99%

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Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway

Zhou

Feng

et al. 2019

CNS Neurosci Ther

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Aims Central diabetes insipidus (CDI), a typical complication caused by pituitary stalk injury, often occurs after surgery, trauma, or tumor compression around hypothalamic structures such as the pituitary stalk and optic chiasma. CDI is linked to decreased arginine vasopressin (AVP) neurons in the hypothalamic supraoptic nucleus and paraventricular nucleus, along with a deficit in circulating AVP and oxytocin. However, little has been elucidated about the changes in AVP neurons in CDI. Hence, our study was designed to understand the role of several pathophysiologic changes such as endoplasmic reticulum (ER) stress and apoptosis of AVP neurons in CDI. Methods In a novel pituitary stalk electric lesion (PEL) model to mimic CDI, immunofluorescence and immunoblotting were used to understand the underlying regulatory mechanisms. Results We reported that in CDI condition, generated by PEL, ER stress induced apoptosis of AVP neurons via activation of the PI3K/Akt and ERK pathways. Furthermore, application of N‐acetylcysteine protected hypothalamic AVP neurons from ER stress‐induced apoptosis through blocking the PI3K/Akt and ERK pathways. Conclusion Our findings showed that AVP neurons underwent apoptosis induced by ER stress, and ER stress might play a vital role in CDI condition through the PI3K/Akt and ERK pathways.

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Section: Methodsmentioning

confidence: 99%

Section: Pituitary Stalk Electric Lesion Surgerymentioning

confidence: 99%

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway

Zhou

Feng

et al. 2019

CNS Neurosci Ther

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“…In addition to playing an important role in the regulation of reproductive, digestive, stress, memory, neuroimmunity and other activities, OT has a close relationship with animal food intake and obesity [1] . OT promotes termination of feeding mainly through a central mechanism [1] .…”

Section: Introductionmentioning

confidence: 99%

“…OT promotes termination of feeding mainly through a central mechanism [1] . At the end of a meal, OT neuronal activity increases and OT release increases due to excessive plasma osmolality and excessive gastric stretch.…”

Section: Introductionmentioning

confidence: 99%

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The Effect of Oxytocin on Food Intake in the Nucleus Accumbens Core

Zhao¹,

Wang²,

Wang³

et al. 2018

ijSciences

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Objective: In this experiment, we mainly investigated whether oxytocin (OT) acts on the Nucleus Accumbens Core (NAc) to regulate the feeding behavior of rats. Methods: Immunohistochemical experiment was used to observe the expression of OT receptor in NAc; To observe the effect of direct injection of OT in NAc on hunger-and palatability-driven food intake; c-Fos expression can be used as a marker of neuronal activation. After the NAc injection ofOT, the expression of c-fos in the hypothalamus was observed. Results: Studies found that the NAc injection of OT significantly reduced fasting-induced food intake and reduced intake of maltose and dextrin in non-fasted rats. Administration of the OTR antagonist L-368899 within NAc completely blocked these effects induced by OT. The results showed that intra-NAc injection of OT significantly activated NAc, as well as the other two brain regions associated with feeding: hypothalamic paraventricular nucleus and supraoptic nucleus. Conclusion: Injection of OT within NAc significantly attenuated starvation and reward-induced feeding behavior.

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Adult neurogenesis of the median eminence contributes to structural reconstruction and recovery of body fluid metabolism in hypothalamic self-repair after pituitary stalk lesion

Zhou²,

Che³

et al. 2022

Cell. Mol. Life Sci.

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Functional ectopic neural lobe increases GAP-43 expression via PI3K/AKT pathways to alleviate central diabetes insipidus after pituitary stalk lesion in rats

Cited by 13 publications

References 22 publications

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway

Endoplasmic reticulum stress induces apoptosis of arginine vasopressin neurons in central diabetes insipidus via PI3K/Akt pathway

The Effect of Oxytocin on Food Intake in the Nucleus Accumbens Core

Adult neurogenesis of the median eminence contributes to structural reconstruction and recovery of body fluid metabolism in hypothalamic self-repair after pituitary stalk lesion

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