Functional Diversity of Microbial Communities in the Soybean (Glycine max L.) Rhizosphere from Free State, South Africa

Ajiboye, Titilope Tinu; Babalola, Olubukola Oluranti

doi:10.3390/ijms23169422

Cited by 7 publications

(3 citation statements)

References 47 publications

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“…On the other hand, many Burkholderia bacteria have the ability to promote plant growth and to act as biofertilizer, such as the B. vietnamiensis strain TVV74, which produced a 22% increase in rice yield as a result of its nitrogen fixation activity ( Van et al, 2000;Parke and Gurian-Sherman, 2001). Some strains of Burkholderia bacteria occupy dominant ecological niches in the endophytic bacterial community, to prevent the reproduction of pathogens (Hallmann et al, 1999;Ajiboye et al, 2022). Some seed-borne Burkholderia bacteria promote growth by producing plant hormones (Kloepper, 1993).…”

Section: Discussionmentioning

confidence: 99%

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Yang

Wang²,

Wan³

et al. 2023

Front. Plant Sci.

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IntroductionPlant pathogens are one of the major constraints on worldwide food production. The antibiotic properties of microbes identified as effective in managing plant pathogens are well documented.MethodsHere, we used antagonism experiments and untargeted metabolomics to isolate the potentially antifungal molecules produced by KJ-34.ResultsKJ-34 is a potential biocontrol bacterium isolated from the rhizosphere soil of rice and can fight multiple fungal pathogens (i.e. Ustilaginoidea virens, Alternaria solani, Fusarium oxysporum, Phytophthora capsica, Corynespora cassiicola). The favoured fermentation conditions are determined and the fermentation broth treatment can significantly inhibit the infection of Magnaporthe oryzae and Botryis cinerea. The fermentation broth suppression ratio is 75% and 82%, respectively. Fermentation broth treatment disrupted the spore germination and led to malformation of hyphae. Additionally, we found that the molecular weight of antifungal products were less than 1000 Da through semipermeable membranes on solid medium assay. To search the potentially antifungal molecules that produce by KJ-34, we used comparative and bioinformatics analyses of fermentation broth before and after optimization by mass spectrometry. Untargeted metabolomics analyses are presumed to have a library of antifungal agents including benzoylstaurosporine, morellin and scopolamine.DiscussionThese results suggest that KJ-34 produced various biological control agents to suppress multiple phytopathogenic fungi and showed a strong potential in the ecological technologies of prevention and protection.

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Section: Discussionmentioning

confidence: 99%

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Yang

Wang²,

Wan³

et al. 2023

Front. Plant Sci.

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“…Despite the vital contributions of microbial communities to rhizosphere engineering, particularly in the face of limited water and nutrient resources, there remains a significant research gap in understanding their structure, composition, and metabolic activities in arid and semiarid regions [14]. These communities contribute significantly to plant growth and hold agricultural potential in nutrient-deficient regions, making their comprehension essential for restoring rangeland ecosystems [15]. Moreover, environmental changes profoundly impact microbial communities, influencing ecosystem resistance and vegetation productivity [16,17].…”

Section: Microbial Community Associated With Arid and Semiarid Region...mentioning

confidence: 99%

Rhizosphere engineering for semiarid tropics: prospects and bottlenecks

Yadav,

Ahmad

2023

Academia Biology

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Rhizosphere engineering is a cutting-edge biotechnological approach, strategically employing microbial biofertilizers, phytostimulants, and plant growth promoting rhizobacteria (PGPR) to boost agricultural crop productivity. Unlike conventional chemical fertilizers, this method eliminates harmful substances, mitigating environmental and health concerns. The foundation of rhizosphere engineering lies in the intricate study of plant-microbe interactions, where soil microorganisms play a pivotal role in nutrient cycling, agricultural waste decomposition, and plant growth stimulation. Rhizosphere engineering shows immense promise in the semiarid tropics, covering around 26% of the Earth's ecology and characterized by water scarcity and high temperatures. Microorganisms found in the rhizosphere, endosphere, and vegetation of arid plants have adapted to harsh environmental conditions, offering valuable resources for biofertilizer and biocontrol research. Their application in enhancing water and nutrient absorption can help alleviate water stress, contributing to sustainable crop production in these regions. However, fully realizing the potential of rhizosphere engineering presents numerous challenges. Identifying beneficial microorganisms, establishing standardized protocols, comprehending complex plantmicrobe-soil interactions, and developing efficient delivery systems for microbial inoculants are among the bottlenecks that must be addressed. These challenges underscore the need for continuous research and innovation in this field. Despite being in its infancy, rhizosphere engineering has already accumulated a wealth of information and insights. By surmounting existing challenges and harnessing the power of PGPR and other rhizosphere microorganisms, rhizosphere engineering may usher in a new era in agriculture, particularly benefiting the water-stressed regions of the semiarid tropics.

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“…The rhizosphere microbial populations have been extensively studied using culture-dependent methodologies (Ajiboye et al, 2022). The drawback of this approach is the inability to sufficiently elucidate the roles of soil microorganisms in a specific environment (Fadiji et al, 2021c).…”

Section: Introductionmentioning

confidence: 99%

Variations in the Functional Diversity of Rhizosphere Microbiome of Healthy and Northern Corn Leaf Blight Infected Maize (Zea mays L.)

Dlamini¹,

Akanmu²,

Babalola³

2023

Span. J. Soil Sci.

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Metagenomics is a scientific breakthrough that can reveal the variations in the microbial diversities and functions between the healthy and diseased plants, towards a productive deployment in diverse biotechnological processes and agricultural activities. This study investigated the possible functional diversity in the rhizosphere microbiome of both healthy and Northern Corn Leaf Blight (NCLB) infected maize growing at farms in the Lichtenburg (LI) and Mafikeng (MA) areas of the North West Province, South Africa. We hypothesized variations in the abundance and diversities of microbial functions in the healthy (LI and MA) and diseased (LID and MAD) maize plants. Hence, we extracted DNA from the healthy and diseased maize rhizosphere in the two maize farms and sequenced using a shotgun approach. Using the SEED subsystem, we discovered that the healthy rhizosphere maize plant was dominated by 24 functional categories, while the NCLB infected rhizosphere maize plant was dominated by 4 functional categories. Alpha diversity analysis showed no significant (p > 0.05) difference between the healthy and diseased maize rhizosphere. However, the analysis of beta diversity showed a significant difference. The substantial abundance of functional groups detected especially in LI indicates that presence of plant diseases altered the functions of soil microbiomes. The significant abundance of the unknown role of rhizosphere microbiomes in disease management suggests the presence of some undiscovered functional genes associated with the microbiome of the healthy maize rhizosphere. Hence, further investigation is needed to explore the roles of these functional genes for their agricultural or biotechnological relevance.

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Functional Diversity of Microbial Communities in the Soybean (Glycine max L.) Rhizosphere from Free State, South Africa

Cited by 7 publications

References 47 publications

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Burkholderia gladioli strain KJ-34 exhibits broad-spectrum antifungal activity

Rhizosphere engineering for semiarid tropics: prospects and bottlenecks

Variations in the Functional Diversity of Rhizosphere Microbiome of Healthy and Northern Corn Leaf Blight Infected Maize (Zea mays L.)

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