Functional Disorder of the Retina in Manganese-Deficient Japanese Quail Revealed by Electroretinography using a Contact Lens Electrode with Built-In Light Source

Endo, Kentaro; Itoh, Norihiko; Maehara, Seiya; Shinozaki, Aya; Imagawa, Tomohiro; Uehara, Masato; Mizuno, Naoharu; Shoji, Shuichi; Hiraga, Takeo

doi:10.1292/jvms.70.139

Cited by 8 publications

(4 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…However, high Mn exposure is also associated with potent neurotoxicity effects. Some studies found that animals fed with Mn-deficient diet for long periods can result in changes both on the morphology and function of the retina or optic nerve [16,17]. Contrast sensitivity was reported to be significantly reduced in heavy smokers compared to non-smokers, and mean serum Mn levels were significantly lower in smokers than in non-smokers [18].…”

Section: Discussionmentioning

confidence: 99%

Mineral Elements in the Hair of Amblyopic Children

Zhu

Chen

Zheng

et al. 2010

Biol Trace Elem Res

View full text Add to dashboard Cite

Amblyopia is a common cause of vision damage in children, and some aspects of its etiology are not clear. A number of mineral elements have important effects on the nerve and visual nerve systems. However, little is known about the relationship between amblyopia and nutritional mineral elements. In this study, hair samples were collected from 67 children with amblyopia and 57 age-matched control groups. The height and weight of each child was measured, and body mass index (BMI) was calculated. Mineral elements were determined by atomic absorption spectrometry and inductively coupled plasma-atomic emission spectrometry. The calcium and magnesium levels in the hair of amblyopic children were higher (p < 0.006), but the level of manganese were lower compared with those in the control groups (p < 0.006). Other elements measured were found to have an insignificant difference between the two groups (p > 0.006). The BMI of amblyopic children was higher (p < 0.001). These results show that mineral elements may play an important role in the visual development of children. Therefore, studies should pay more attention to investigating the impact of mineral elements on child vision.

show abstract

Section: Discussionmentioning

confidence: 99%

Mineral Elements in the Hair of Amblyopic Children

Zhu

Chen

Zheng

et al. 2010

Biol Trace Elem Res

View full text Add to dashboard Cite

show abstract

“…11,12 Trace elements deficiency, undernourishment, and exposure to drugs of abuse or alcohol induced ultrastructural changes in the ON, cornea, and retina. 25,26,29,[31][32][33][34][35][42][43][44][45][46][47][48][49][50][51][52][53] Figure 2 Mean density (a) and total number (b) of myelinated and unmyelinated fibres of the optic nerve in the control, anaemic, and recovered rats at postnatal day 32. Values are means ± SE for n ¼ 6; *Po0.05.…”

Section: Discussionmentioning

confidence: 99%

Effectiveness of iron repletion in the diet for the optic nerve development of anaemic rats

Demaman

Melo

Homem

et al. 2009

Eye

View full text Add to dashboard Cite

Purpose To compare the process of myelination in the developing optic nerve (ON) of anaemic rats with the subsequent recovery after being fed an iron-recovery diet. Methods In this study, the morphometrical parameters in the ON were assessed by electron microscopy in Wistar rats that were on an iron-deficient diet for 32 days or for 21 days followed by 10 days on an iron-recovery diet. Qualitative and quantitative analyses were performed using representative electron ultramicrographs. Data were analysed by one-way analysis of variance (ANOVA). When differences were detected, comparisons were made using Tukey's post hoc test (Po0.05 was considered to be significant). Results Qualitative analysis of the ONs in anaemic and recovered animals showed a higher rate of deformed axons and increased lamellar separation in the myelin sheath when compared with the respective control group. The ON of the anaemic group showed a reduced mean density of myelinated fibres when compared with the control group. The fibre area ratio, axon area ratio, and myelin area ratio of large axons/small axons in the ONs of the control group showed the highest values for the myelin areas, axon areas, and total fibre areas. The control group showed a significantly higher myelin sheath thickness when compared with the anaemic and recovered groups. Conclusions Our data indicate that iron is necessary for maintenance of the ON cell structure, and that morphological damage from iron deficiency is not easily reverted by iron repletion.

show abstract

“…We evaluated a simple, easy to build and inexpensive experimental setup that includes contact lens electrodes with a built-in white light-emitting diode (LED) light source for ERG recording in rats. This type of electrode has been used in humans (Suzuki et al 1998, Tsuruoka et al 2004, dogs (Kim et al 2008, Yu et al 2007, Sasaki et al 2006, Maehara et al 2005, quail (Endo et al 2008) and rabbits (Maia et al 2004). There are four reports in the literature that describe its use in rats, but with a different ERG setup (Goto et al 2002, Grozdanic et al 2003, Yoneda et al 2003, Yamashita et al 2009.…”

Section: Introductionmentioning

confidence: 99%

Monitoring the effect of mild ischemia with a built-in light-emitting diode contact lens electrode and a low-cost custom-made apparatus

Fleischman¹,

Parvari²,

Oron³

et al. 2012

Physiol. Meas.

View full text Add to dashboard Cite

Electroretinography (ERG) is widely used in clinical work and research to assess the retinal function. We evaluated an easy to build ERG setup adapted for small animals comprising two contact lens electrodes with a built-in light-emitting diode and a custom-made amplification system. The system's sensitivity was tested by monitoring ERG in albino rat eyes subjected to mild ischemia. Flash ERG was recorded by two contact lens electrodes positioned on the rat's corneas and used alternately as test or reference. The a- and b-wave amplitudes, a-wave latency, b-wave implicit time and oscillatory potentials (OPs) were analyzed. Ischemia was achieved by elevating the intraocular pressure in the eye's anterior chamber. ERG was recorded on post-ischemia (PI) days -1, 1, 3 and 7. Morphological changes were analyzed on hematoxylin/eosin stained 5 µm sections of control 7d PI retinas. In control eyes, ERG exhibited a pattern similar to a standard recording. Retinas subjected to mild ischemia preserved ordered layered morphology, exhibiting approximately 30% loss of ganglion cells and no changes in gross morphology. By day 3 PI, ischemia caused an increase in the a-wave amplitude (from 34.9 ± 2.7 to 45.4 ± 4.3 µV), a decrease in the b-wave amplitude (from 248 ± 13 to 162 ± 8 µV), an increase in a-wave latency (from 11.1 ± 0.3 to 17.3 ± 1.4 ms) and b-wave implicit time (from 81.0 ± 1.6 to 90.0 ± 2.5 ms), and attenuation of OPs. The described setup proved sensitive and reliable for evaluating subtle changes in the retinal function in small animals.

show abstract

Functional Disorder of the Retina in Manganese-Deficient Japanese Quail Revealed by Electroretinography using a Contact Lens Electrode with Built-In Light Source

Cited by 8 publications

References 21 publications

Mineral Elements in the Hair of Amblyopic Children

Mineral Elements in the Hair of Amblyopic Children

Effectiveness of iron repletion in the diet for the optic nerve development of anaemic rats

Monitoring the effect of mild ischemia with a built-in light-emitting diode contact lens electrode and a low-cost custom-made apparatus

Contact Info

Product

Resources

About