Functional differentiation of endosomes in Arabidopsis cells

Ueda, Takashi; Uemura, Tomohiro; Sato, Masa H.; Nakano, Akihiko

doi:10.1111/j.1365-313x.2004.02249.x

Cited by 246 publications

(275 citation statements)

References 30 publications

(35 reference statements)

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“…The membrane-selective fluorescent FM4-64 dye is a reliable marker to analyze the global endocytosis phenomenon and to visualize endosomes in plant cells (Bolte et al, 2004;Ueda et al, 2004). Real-time monitoring of FM4-64 internalization indicates that the rate of global endocytosis is higher in cryptogeinelicited cells at least in the first 15 min after treatment.…”

Section: Discussion Endocytosis As An Early Signaling Event Elicitedmentioning

confidence: 99%

The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells

et al. 2008

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The plant defense elicitor cryptogein triggers well-known biochemical events of early signal transduction at the plasma membrane of tobacco (Nicotiana tabacum) cells, but microscopic observations of cell responses related to these early events were lacking. We determined that internalization of the lipophilic dye FM4-64, which is a marker of endocytosis, is stimulated a few minutes after addition of cryptogein to tobacco Bright Yellow-2 (BY-2) cells. This stimulation is specific to the signal transduction pathway elicited by cryptogein because a lipid transfer protein, which binds to the same receptor as cryptogein but without triggering signaling, does not increase endocytosis. To define the nature of the stimulated endocytosis, we quantified clathrin-coated pits (CCPs) forming on the plasma membrane of BY-2 cells. A transitory stimulation of this morphological event by cryptogein occurs within the first 15 min. In the presence of cryptogein, increases in both FM4-64 internalization and clathrin-mediated endocytosis are specifically blocked upon treatment with 5 mM tyrphostin A23, a receptor-mediated endocytosis inhibitor. The kinetics of the transient increase in CCPs at the plasma membrane coincides with that of transitory reactive oxygen species (ROS) production occurring within the first 15 min after elicitation. Moreover, in BY-2 cells expressing NtrbohD antisense cDNA, which are unable to produce ROS when treated with cryptogein, the CCP stimulation is inhibited. These results indicate that the very early endocytic process induced by cryptogein in tobacco is due, at least partly, to clathrin-mediated endocytosis and is dependent on ROS production by the NADPH oxidase NtrbohD.

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Section: Discussion Endocytosis As An Early Signaling Event Elicitedmentioning

confidence: 99%

The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells

et al. 2008

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“…Rab GTPases are key regulators of vesicular transport and vesicle fusion (Zerial and McBride, 2001). In the protoplasts of Arabidopsis suspension-cultured cells, AtRABF1 and AtRABF2b were located to two distinct populations of endosomes, with some overlap (Ueda et al, 2001(Ueda et al, , 2004. By following internalization of the fluorescent lipophilic styryl dye FM4-64, AtRABF1 was localized mainly to a late type of endosome, whereas AtRABF2b was localized on early endosomes (Ueda et al, 2004).…”

Section: Introductionmentioning

confidence: 98%

“…To date, only a few endosomal proteins have been described in plants, and endosome differentiation and functions are not well defined. The first plant proteins shown to associate with the membrane of endosomes were the Arabidopsis Rab GTPases AtRABF1 (also known as Ara6) and AtRABF2b (also known as Ara7) (Ueda et al, 2001(Ueda et al, , 2004. RABF1 and RABF2b are two of three Arabidopsis homologues of the mammalian small GTPase Rab5.…”

Section: Introductionmentioning

confidence: 99%

Evidence for a sorting endosome in Arabidopsis root cells

et al. 2007

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SummaryIn eukaryotic cells, the endocytic and secretory pathways are key players in several physiological processes. These pathways are largely inter-connected in animal and yeast cells through organelles named sorting endosomes. Sorting endosomes are multi-vesicular compartments that redirect proteins towards various destinations, such as the lysosomes or vacuoles for degradation, the trans-Golgi network for retrograde transport and the plasma membrane for recycling. In contrast, cross-talk between the endocytic and secretory pathways has not been clearly established in plants, especially in terms of cargo protein trafficking. Here we show by co-localization analyses that endosomes labelled with the AtSORTING NEXIN1 (AtSNX1) protein overlap with the pre-vacuolar compartment in Arabidopsis root cells. In addition, alteration of the routing functions of AtSNX1 endosomes by drug treatments leads to mis-routing of endocytic and secretory cargo proteins. Based on these results, we propose that the AtSNX1 endosomal compartment represents a sorting endosome in root cells, and that this specialized organelle is conserved throughout eukaryotes.

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“…Therefore, colocalization of proteins with internalized FM4-64 may be used to judge whether a particular compartment labeled by a specific protein is a putative endosomal compartment in plant cells. Proteins localized to plant endosomes in this way include the Arabidopsis thaliana Rab GTPases ARA6 and ARA7 (Ueda et al, 2001(Ueda et al, , 2004, Arabidopsis PRA2 (SYP111) (Inaba et al, 2002), the small GTPase ARF1 in maize (Zea mays) (Baluska et al, 2002, the Arabidopsis GNOM protein and the auxin-efflux carrier PIN1 (Geldner et al, 2003), and vacuolar H þ -ATPase (Dettmer et al, 2006). However, in the great majority of these cases, the ultrastructural morphology of the compartments bearing these proteins was not given.…”

Section: Introductionmentioning

confidence: 99%

Rice SCAMP1 Defines Clathrin-Coated,trans-Golgi–Located Tubular-Vesicular Structures as an Early Endosome in Tobacco BY-2 Cells

et al. 2007

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We recently identified multivesicular bodies (MVBs) as prevacuolar compartments (PVCs) in the secretory and endocytic pathways to the lytic vacuole in tobacco (Nicotiana tabacum) BY-2 cells. Secretory carrier membrane proteins (SCAMPs) are post-Golgi, integral membrane proteins mediating endocytosis in animal cells. To define the endocytic pathway in plants, we cloned the rice (Oryza sativa) homolog of animal SCAMP1 and generated transgenic tobacco BY-2 cells expressing yellow fluorescent protein (YFP)-SCAMP1 or SCAMP1-YFP fusions. Confocal immunofluorescence and immunogold electron microscopy studies demonstrated that YFP-SCAMP1 fusions and native SCAMP1 localize to the plasma membrane and mobile structures in the cytoplasm of transgenic BY-2 cells. Drug treatments and confocal immunofluorescence studies demonstrated that the punctate cytosolic organelles labeled by YFP-SCAMP1 or SCAMP1 were distinct from the Golgi apparatus and PVCs. SCAMP1-labeled organelles may represent an early endosome because the internalized endocytic markers FM4-64 and AM4-64 reached these organelles before PVCs. In addition, wortmannin caused the redistribution of SCAMP1 from the early endosomes to PVCs, probably as a result of fusions between the two compartments. Immunogold electron microscopy with high-pressure frozen/freeze-substituted samples identified the SCAMP1-positive organelles as tubular-vesicular structures at the trans-Golgi with clathrin coats. These early endosomal compartments resemble the previously described partially coated reticulum and trans-Golgi network in plant cells.

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Functional differentiation of endosomes in Arabidopsis cells

Cited by 246 publications

References 30 publications

The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells

The Plant Defense Elicitor Cryptogein Stimulates Clathrin-Mediated Endocytosis Correlated with Reactive Oxygen Species Production in Bright Yellow-2 Tobacco Cells

Evidence for a sorting endosome in Arabidopsis root cells

Rice SCAMP1 Defines Clathrin-Coated,trans-Golgi–Located Tubular-Vesicular Structures as an Early Endosome in Tobacco BY-2 Cells

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