Functional differentiation of bmp2a and bmp2b genes in zebrafish

Wu, Yaming; Sun, Aili; Nie, Chun‐Hong; Gao, Zexia; Wan, Shiming

doi:10.1016/j.gep.2022.119288

Cited by 5 publications

(3 citation statements)

References 39 publications

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“…These subsequent generational effects, mediated by mothers and fathers exposed to toxicants, may occur due to the bioaccumulation and transfer of toxicants in adult zebrafish germ cells. However, there is evidence that developmental changes occur through epigenetic modification of genes transferred from F0 to F1 larvae (67). Thus, complementary transgenerational studies are required to verify whether the transcriptional changes induced by caffeine can be passed on to subsequent generations.…”

Section: Discussionmentioning

confidence: 99%

Caffeine exposure causes downregulation of the expression of genes related to osteogenesis and chondrogenesis in Zebrafish (Danio rerio)

Bertassoli,

Santana,

Nogueira

et al. 2024

BJVP

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With the aim of evaluating the effects of caffeine on the expression of gene transcripts related to osteogenesis and chondrogenesis, during zebrafish embryonic development the embryos were divided into five groups: control (without caffeine), 0.25 mM caffeine, 0.5 mM caffeine, 1.0mM caffeine, and 2.0mM caffeine. Embryos were placed in cell culture plates with aquarium water at 280 C for 72 hours post-fertilization (hpf). Embryonic development was followed at times of six, 12 hpf, 24 hpf, 30 hpf, 48 hpf, 54 hpf, and 72 hpf. At 72 hpf, the expression of Sox9a, runx2b, osteocalcin, osteopontin, collagen 2a1, and bmp2a was analyzed using real-time RT-PCR. The 0.25mM caffeine group showed embryonic development that was visually similar to the control group despite having a significantly lower relative expression of bmp2a, runx2b, and collagen 2a1. In the 0.5mM and 1mM caffeine groups, morphological alterations such as tail folding, pericardial edema, and yolk sac deformation were observed, and the relative expression levels of bmp2a, runx2b, and collagen 2a1 were significantly lower. In contrast, osteocalcin expression was significantly higher than in the control group. In the 2mM caffeine group, the embryos did not hatch until 72 hpf and showed tail docking and pericardial and yolk sac edema. The relative expression of bmp2a and runx2b was significantly lower, whereas that of osteocalcin, osteopontin, and collagen 2a1 did not differ from that of the control group. We conclude that caffeine altered the expression of gene transcripts related to osteogenesis and chondrogenesis in zebrafish embryos, even in embryos without visible morphological changes.

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Section: Discussionmentioning

confidence: 99%

Caffeine exposure causes downregulation of the expression of genes related to osteogenesis and chondrogenesis in Zebrafish (Danio rerio)

Bertassoli,

Santana,

Nogueira

et al. 2024

BJVP

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“…embryos and adults. BMP plays a crucial role in the formation of the body axis, particularly in the development of the ventral side during early life stages [46]. NP's effects on embryos can lead to the downregulation of BMP2 and BMP4 expression, resulting in morphological changes such as the abnormal development of tail bones during the early life stages [30].…”

Section: Discussionmentioning

confidence: 99%

Mechanistic Insights into Nonylphenol Stress on BMP2 and BMP4 Gene Expression in Red Crucian Carp (Carassius auratus Red var.)

Li,

Cui,

Chen

et al. 2024

Fishes

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Nonylphenol (NP) is a known endocrine-disrupting chemical (EDC) that has been shown to affect bone development in mammals. However, the detrimental impacts of NP on the skeletal growth and development of aquatic species, especially bony fish, remain poorly understood. Bone morphogenic proteins (BMPs), essential for bone formation and osteoblast differentiation, act through the BMP-Smad signaling pathway. In this study, two BMP genes, BMP2 and BMP4, were cloned and characterized in the red crucian carp (Carassius auratus red var.). The full-length cDNAs of BMP2 and BMP4 were 2029 bp and 2095 bp, respectively, encoding polypeptides of 411 and 433 amino acids, and share a typical TGF-β domain with other BMPs. The tissue expression patterns of both genes were identified, showing ubiquitous expression across all studied tissues. Additionally, the exposure of embryos or adult fish to NP stress resulted in a downregulation of BMP2, BMP4, and other genes associated with the BMP-Smad signaling pathway. Moreover, the combined treatment of adult fish with NP and the specific BMP receptor inhibitor significantly reduced these genes’ expression. These findings elucidate the mechanism of NP stress on BMP2 and BMP4, suggesting a role for the BMP-Smad signaling pathway in the response to endocrine-disrupting chemicals in fish.

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“…The genes wnt16 [ 233 ], hoxaa , hoxab , hoxba , hoxca , hoxda , [ 234 ], bmp2a , bmp2b [ 235 ], and nkx3.2 [ 175 ] control the development of the spine, skull, and fins. Mutants for these genes exhibited significant deformities in body shape.…”

Section: Genome Editing In Salmonidae and Cyprinidae Aquaculture Fish...mentioning

confidence: 99%

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

Orlova,

Ruzina,

Emelianova

et al. 2024

Genes

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Aquaculture supplies the world food market with a significant amount of valuable protein. Highly productive aquaculture fishes can be derived by utilizing genome-editing methods, and the main problem is to choose a target gene to obtain the desirable phenotype. This paper presents a review of the studies of genome editing for genes controlling body development, growth, pigmentation and sex determination in five key aquaculture Salmonidae and Cyprinidae species, such as rainbow trout (Onchorhynchus mykiss), Atlantic salmon (Salmo salar), common carp (Cyprinus carpio), goldfish (Carassius auratus), Gibel carp (Carassius gibelio) and the model fish zebrafish (Danio rerio). Among the genes studied, the most applicable for aquaculture are mstnba, pomc, and acvr2, the knockout of which leads to enhanced muscle growth; runx2b, mutants of which do not form bones in myoseptae; lepr, whose lack of function makes fish fast-growing; fads2, Δ6abc/5Mt, and Δ6bcMt, affecting the composition of fatty acids in fish meat; dnd mettl3, and wnt4a, mutants of which are sterile; and disease-susceptibility genes prmt7, gab3, gcJAM-A, and cxcr3.2. Schemes for obtaining common carp populations consisting of only large females are promising for use in aquaculture. The immobilized and uncolored zebrafish line is of interest for laboratory use.

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Functional differentiation of bmp2a and bmp2b genes in zebrafish

Cited by 5 publications

References 39 publications

Caffeine exposure causes downregulation of the expression of genes related to osteogenesis and chondrogenesis in Zebrafish (Danio rerio)

Caffeine exposure causes downregulation of the expression of genes related to osteogenesis and chondrogenesis in Zebrafish (Danio rerio)

Mechanistic Insights into Nonylphenol Stress on BMP2 and BMP4 Gene Expression in Red Crucian Carp (Carassius auratus Red var.)

In Search of a Target Gene for a Desirable Phenotype in Aquaculture: Genome Editing of Cyprinidae and Salmonidae Species

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