Functional Development of Oligodendrocytes and Open-field Behavior in Developing Rats: An Approach Using Monoclonal Antibody to Immature Oligodendrocytes.

Abstract

Abstract:To examine the relation between functional development of oligodendrocytes and open-field behavior during the postnatal period, a mouse monoclonal antibody termed 14F7, which predominantly labels stage-specific immature oligodendrocytes, was employed. Antibody 14F7 was administered intraperitoneally into male pups on day 3 and 4 after birth. The open-field test was performed on days 12 and 18 of the postnatal period. Horizontal activity increased remarkably with the growth of pups. On day 18, horizontal activity in the group with 14F7 was significantly higher than the control, while there was no significant difference between treatments on day 12. In contrast to the horizontal activity, the frequency of hind leg rearing, vertical activity, in the group with 14F7 was significantly lower than that in the control. On day 12, choline acetyltransferase (ChAT) and acetylcholinesterase (AChE) activities in the cerebral cortex were similar between the groups. These activities increased with the growth of pups in both groups. In the 14F7 group on day 18, ChAT activity was the same as the control, whereas AChE activity was significantly lower compared with the control. These results suggest that neonatal exposure to 14F7 induces abnormal neurotransmission by reducing the degradation of acetylcholine and alters the spontaneous activities in developing rats.

Protective effect of walnut on d‐galactose‐induced aging mouse model

“…Movement distance and movement path in the middle region could infer the mouse's cognitive and exploration capabilities in the mine experiment. Under normal circumstances, animals would actively explore the new environment, but animals with poor cognitive ability would have less activity in the new environment (Yamamuro, Yoshimura, Tsuchiya, Sensui, & Asou, ). The grooming behavior of animals appeared in a more comfortable environment and a leisure activity in a new environment, indicating that mice were more adaptable in an unfamiliar environment (Zhang et al., ).…”