Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae)

Carvalho, Vanda Marilza de; Marques, Rosimar Maria; Lapenta, Ana Sílvia; Machado, Maria de Fátima Pires da Silva

doi:10.1590/s1415-47572003000200013

Cited by 31 publications

(27 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…So plant-esterase can be widely applied in rapid warning alerts and field analysis for OPs (Huo et al 2009). Several methods have been developed for the purification of plant-esterase by different researchers (Cummins and Edwards 2004;De Carvalho et al 2003;Stuhlfelder et al 2002). However, almost all these methods involve a large number of steps such as precipitation, centrifugation, dialysis, ion-exchange chromatography and gel filtration chromatography which take higher loss of product yield (Kula et al 1992).…”

Section: Introductionmentioning

confidence: 99%

Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

et al. 2014

View full text Add to dashboard Cite

To explore a new and simple rapid extraction and purification technique for wheat-esterase, an ionic liquids (ILs)-based aqueous two-phase system (ATPS) was developed for the purification of wheat-esterase from wheat extracts. Effects of various process parameters such as the concentrations of [Bmim]BF 4 , the types and concentrations of phaseforming salt, the system pH and the temperature on partitioning of wheat-esterase were evaluated. The obtained data indicated that wheat-esterase was preferentially partitioned into the ILs-rich phase and the ATPS composed of 20 % [Bmim]BF 4 (w/w) and 25 % (w/w) NaH 2 PO 4 (pH= 4.8) showed good selectivity on wheat-esterase. Under the optimum conditions, wheat-esterase was purified with an acceptable yield (88.93 %), but produced wheat-esterase was 4.23 times as pure. It was obvious that temperature shows little influence on the purification between 10 and 50°C. Sephadex G-150FF revealed that the band intensity of contaminating proteins in ATPS fraction almost disappeared. Therefore, ILsbased ATPS was an effective method for partitioning and recovery of wheat-esterase from wheat crude extracts.

show abstract

Section: Introductionmentioning

confidence: 99%

Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Most esterase isozymes in plant tissues have been classified as carboxylesterases (Pereira et al, 2001;Carvalho et al, 2003;Orasmo et al, 2007). The carboxylesterase activity in plants has been correlated with differentiation processes (Gahan et al, 1983;Melati et al, 1996), and different physiological roles have recently been attributed to these compounds.…”

Section: Discussionmentioning

confidence: 99%

“…Polyacrylamide gel electrophoresis (PAGE) has been used to analyze α-and β-esterase genetic polymorphism in plants (Pereira et al, 2001;Carvalho et al, 2003;Orasmo et al, 2007;Frigo et al, 2009) and is also useful for analyses of the genetic diversity and structure of Capsicum accessions maintained at BAGC. Esterases are often found in multigene families (Robin et al, 1996), and a large number of esterase loci in plant tissues can be used simultaneously for the identification of genetic variation and polymorphism analyses.…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity analysis of Capsicum spp germplasm bank accessions based on α/β-esterase polymorphism

Monteiro

Bronzato²,

Orasmo³

et al. 2013

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. Genetic diversity and structure were analyzed in 10 accessions belonging to Banco Ativo de Germoplasma de Capsicum located at Federal University of Piauí in northwestern Brazil that receives pepper samples grown in community gardens in various regions and Brazilian states. Selections were made from seeds of C. chinense (4 accessions), C. annuum (5 accessions), and C. baccatum (1 accession). Samples consisting of leaves were collected from 4-10 plants of each accession (a total of 85 plants). Native polyacrylamide gel electrophoresis was used to identify α-and β-esterase polymorphisms. Polymorphism was clearly detected in 5 loci. Sixteen alleles were found at 5 α/β-esterase loci of the three Capsicum species. In the C. chinense samples, the highest H O and H E values were 0.3625 and 0.4395, respectively, whereas in C. annuum samples, H O and H E values were 0.2980 and 0.3310, respectively; the estimated H O and H E values in C. chinense samples were higher than those detected in C. annuum samples. A deficit of homozygous individuals was found in C. chinense (F IS = -0.6978) and C. annuum (F IS = 0.7750). Genetic differentiation between C. chinense and C. annuum at these loci was high (F ST = 0.1867) indicating that C. chinense and C. annuum are genetically structured species for α/β-esterase isozymes. The esterase analysis showed high genetic diversity among the C. chinense and C. annuum samples and very high genetic differentiation (F ST = 0.6321) among the C. chinense and C. annuum samples and the C. baccatum accession.

show abstract

“…They are adequate co-dominant markers for the analysis of genetic structure in populations. Esterases are often found in multigene families (Oakeshott et al, 1993;Robin et al, 1996); 14 to 16 esterase isozymes have been detected by polyacrylamide gel electrophoresis (PAGE) in different plant species (Pereira et al, 2001;Carvalho et al, 2003;Orasmo et al, 2007;Frigo et al, 2009). A large number of esterase loci can be used simultaneously for the identification of genetic variation and for polymorphism analyses.…”

Section: Introductionmentioning

confidence: 99%

“…The PAGE system has been used for analysis of genetic diversity in peroba trees (Aspidosperma polyneuron; Carvalho et al, 2003), cassava (Manihot esculenta; Pereira et al, 2001), grapes (Vitis vinifera; Orasmo et al, 2007), and wild poinsettia (Euphorbia heterophylla; Frigo et al, 2009), but no such studies have been made of soybean (Glycine max) cultivars. Genetic diversity in soybean has been assessed using morphological traits (Perry et al, 1991), pedigrees (Zhou et al, 2000), geographic origins (Gorman, 1984), parentage coefficient (Miranda et al, 2007), isozymes (Yu and Kiang, 1993;Griffin and Palmer, 1995;Hirata et al, 1999), and DNA markers (Abdelnoor et al, 1995;Li and Nelson, 2001;Ude et al, 2003;Xu and Gai, 2003;Priolli et al, 2002Priolli et al, , 2004Bonato et al, 2006;Yamanaka et al, 2007;Fu et al, 2007;Hwang et al, 2008).…”

Section: Introductionmentioning

confidence: 99%

Esterase polymorphisms for analysis of genetic diversity and structure of soybean (Glycine max) cultivars

Mangolin¹,

Collet²,

Machado³

2011

Genet. Mol. Res.

View full text Add to dashboard Cite

ABSTRACT. We used native polyacrylamide gel electrophoresis to identify polymorphism levels in a-and b-esterase loci from leaf tissues of Brazilian soybean cultivars for the analysis of population genetic diversity and structure, and to investigate relationships between conventional and genetically modified cultivars. The cultivars included lines developed by a soybean-grower cooperative (CD), by EMBRAPA (BR), and "Roundup Ready" (RR) cultivars. Esterase isozymes recorded with α-naphthyl acetate and β-naphthyl acetate were produced from 14 loci. Two to three allelic variants were detected in leaves from 420 plants of 21 CD, BR, and RR cultivars at Est-1, Est-2, Est-3, Est-5, and Est-14 loci. The estimated proportion of polymorphic loci in CD cultivars was 21.4%, and in BR and RR cultivars it was 28.6%. High and low H O and H E values were observed within CD and BR cultivars and a very high cultivar differentiation level was evident in the plants of the 21 CD, BR, and RR cultivars (F ST = 0.3865). A low level of differentiation (F ST = 0.0289) was detected between conventional and RR cultivars. Plants from cultivar BR37 had the highest level of genetic differentiation compared to the other cultivars. The genetic basis of BR cultivars (0.5538-0.9748) was found to be broader than the genetic Analysis of genetic diversity and structure of Glycine max basis of CD cultivars (0.7058 for CD205 and CD209 and 0.9995 for CD205 and CD208). Higher genetic identity was detected between plants of CD and CDRR cultivars (I = 0.9816). Understanding the genetic structure of these populations can help provide specific culture strategies for each cultivar, depending on its level of heterozygosity.

show abstract

Functional classification of esterases from leaves of Aspidosperma polyneuron M. Arg. (Apocynaceae)

Cited by 31 publications

References 26 publications

Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

Extraction and purification of wheat-esterase using aqueous two-phase systems of ionic liquid and salt

Genetic diversity analysis of Capsicum spp germplasm bank accessions based on α/β-esterase polymorphism

Esterase polymorphisms for analysis of genetic diversity and structure of soybean (Glycine max) cultivars

Contact Info

Product

Resources

About