Functional cholesteryl binding agents: synthesis, characterization, and evaluation of antibody binding to modified phospholipid vesicles

Carroll, Timothy R.; Davison, Alan; Jones, Alun G.

doi:10.1021/jm00160a004

Cited by 13 publications

(2 citation statements)

References 3 publications

(7 reference statements)

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“…In this way the antibody was covalently bound to the outside of the vesicle. 45 In this study the N-hydroxysuccinimide esters were shown to be better than cholesterol derivatives with isothiocyanate or maleimide reactive end groups. Only the N-hydroxysuccinimide esters gave protein binding which was significantly higher than background nonspecific binding.…”

Section: Uses Of the Hydroxysuccinimide Derivatives 6 Andmentioning

confidence: 75%

Cholesterol-based anchors and tethers for phospholipid bilayers and for model biological membranes

2010

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This review covers the range of cholesterol-based anchors and tethers and the ways in which they are being used. These cholesterol conjugates provide us with a very flexible 'tool-box' that can be used to tether phospholipid bilayers to surfaces, to join bilayers together (bilayer-to-bilayer, bilayer-to-vesicle, vesicle-to-vesicle, etc.) or to anchor molecules, biomolecules, macromolecules or particulate species to the surface of the bilayer. Model biomembranes tethered to a solid support provide a stable platform for addressing membrane components in vitro using force field and spectroscopic methods and since these membranes generally remain fluid and retain much of their biological activity, solid-supported membranes can also be use to study aspects of membrane biology and biochemistry. Potential medical applications are developing out of our ability to anchor 'markers' and antibodies to phospholipid vesicles. Compared to anchors in which the anchoring group is a phospholipid (or phospholipid-like) moiety, cholesterol-based anchors are generally easier to make and purify but the anchoring is less strong and this can be an issue when the 'payload' is large and water-soluble. In the case of nucleic acid functionalised systems this problem has been addressed by anchoring each oligonucleotide through two cholesteryl end-groups.

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Section: Uses Of the Hydroxysuccinimide Derivatives 6 Andmentioning

confidence: 75%

Cholesterol-based anchors and tethers for phospholipid bilayers and for model biological membranes

2010

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“…Thus at pH 8e9, lipid vesicles functionalized with an N-hydroxy succinimido ester react with human IgG, binding the antibody to the outside of the vesicle. 8 Using tethers bearing a maleimide head-group 9e11 it is possible to bind proteins that contain free eSH residues. Maleimide containing tethers have been used to create 'immunoliposomes' using an antibody that targets the epidermal growth factor receptor that is over expressed in brain tumor cells.…”

Section: Introductionmentioning

confidence: 99%