Functional characterization of three ethylene response factor genes from Bupleurum kaoi indicates that BkERFs mediate resistance to Botrytis cinerea

Yu, Wen; Chiou, Shu-Jiau; Ko, Chih‐Yuan; Lin, Tsai-Yun

doi:10.1016/j.jplph.2010.07.006

Cited by 23 publications

(15 citation statements)

References 21 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…In previous studies, our group also showed that two ERFs from Gossypium barbadense , showed positive effects on disease resistance [10], [11]. ERF genes from other species, such as tomato ( TSRF1 and Pti4 ), soybean ( GmERF3 ) and Bupleurum kaoi ( BkERF1 , BkERF2.1 and BkERF2.2 ) also showed positive effects on disease resistance, suggesting the ERF genes have a conserved role in diverse species to counteract with plant pathogens [5], [12]–[16].…”

Section: Introductionmentioning

confidence: 62%

AaERF1 Positively Regulates the Resistance to Botrytis cinerea in Artemisia annua

Jiang

Zhang

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

Plants are sessile organisms, and they can not move away under abiotic or biotic stresses. Thus plants have evolved a set of genes that response to adverse environment to modulate gene expression. In this study, we characterized and functionally studied an ERF transcription factor from Artemisia annua, AaERF1, which plays an important role in biotic stress responses. The AaERF1 promoter had been cloned and GUS staining results of AaERF1 promoter-GUS transgenic A. annua showed that AaERF1 is expressed ubiquitiously in all organs. Several putative cis-acting elements such as W-box, TGA-box and Py-rich element, which are involved in defense responsiveness, are present in the promoter. The expression of AaERF1 can be induced vigorously by methyl jasmonate as well as by ethephon and wounding, implying that AaERF1 may activate some of the defense genes via the jasmonic acid and ethylene signaling pathways of A. annua. The results of electrophoretic mobility shift assay (EMSA) and yeast one-hybrid experiments showed that AaERF1 was able to bind to the GCC box cis-acting element in vitro and in yeast. Ectopic expression of AaERF1 could enhance the expression levels of the defense marker genes PLANT DEFENSIN1.2 (PDF1.2) and BASIC CHITINASE (ChiB), and increase the resistance to Botrytis cinerea in the 35S::AaERF1 transgenic Arabidopsis. The down-regulated expression level of AaERF1 evidently reduced the resistance to B. cinerea in A. annua. The overall results showed that AaERF1 positively regulated the resistance to B. cinerea in A. annua.

show abstract

Section: Introductionmentioning

confidence: 62%

AaERF1 Positively Regulates the Resistance to Botrytis cinerea in Artemisia annua

Jiang

Zhang

et al. 2013

PLoS ONE

View full text Add to dashboard Cite

show abstract

“…The 147 members of the Arabidopsis AP2/ERF superfamily [93,94] have been divided into four families: ethylene response factor (ERF), dehydration-responsive element-binding protein (DREB), apetala (AP2), related to ABI3/VP1 (RAV) and a solo gene, At4g13040. The ERF genes isolated from B. kaoi shares high similarity with the Arabidopsis ERF genes, and their deduced peptides all contain a single AP2/ERF domain [94,95]. The AP2/ERF domain is involved in diverse functions in cellular processes, including hormonal signal transduction [96] and responds to biotic [97] and abiotic stress [93,98].…”

Section: Functional Characterization Of Erf Genes Extracted From Bmentioning

confidence: 99%

“…Overexpression of ERF proteins in Arabidopsis plants led to increased expression of PDF1.2, ChiB and Thi2.1 and increased resistance to both necrotrophic [99,101] and biotrophic pathogens [100]. Enhancement of disease resistance in plants has been achieved by overexpressing ERF proteins, such as Arabidopsis AtERF1 [101,102], AtERF2 [100] and B kaoi BkERF1/2.2 [95]. The rapid activation of BkERF transcription after MeJA treatment may lead to the expression of a subset of defense genes [95].…”

Section: Functional Characterization Of Erf Genes Extracted From Bmentioning

confidence: 99%

“…Enhancement of disease resistance in plants has been achieved by overexpressing ERF proteins, such as Arabidopsis AtERF1 [101,102], AtERF2 [100] and B kaoi BkERF1/2.2 [95]. The rapid activation of BkERF transcription after MeJA treatment may lead to the expression of a subset of defense genes [95]. …”

Section: Functional Characterization Of Erf Genes Extracted From Bmentioning

confidence: 99%

“…Our previous studies demonstrated that B. kaoi ERFs activate defense genes and that the increased gene expression correlates with increased resistance to Botrytis cinerea [95]. The expression of ERF genes in both homologous and heterologous transgenic systems suggests that these genes might be generally useful in conferring increased disease resistance to diverse plant species.…”

Section: Functional Characterization Of Erf Genes Extracted From Bmentioning

confidence: 99%

See 2 more Smart Citations

Putative Genes Involved in Saikosaponin Biosynthesis in Bupleurum Species

Lin

Chiou

2013

IJMS

Self Cite

View full text Add to dashboard Cite

Alternative medicinal agents, such as the herb Bupleurum, are increasingly used in modern medicine to supplement synthetic drugs. First, we present a review of the currently known effects of triterpene saponins-saikosaponins of Bupleurum species. The putative biosynthetic pathway of saikosaponins in Bupleurum species is summarized, followed by discussions on identification and characterization of genes involved in the biosynthesis of saikosaponins. The purpose is to provide a brief review of gene extraction, functional characterization of isolated genes and assessment of expression patterns of genes encoding enzymes in the process of saikosaponin production in Bupleurum species, mainly B. kaoi. We focus on the effects of MeJA on saikosaponin production, transcription patterns of genes involved in biosynthesis and on functional depiction.

show abstract

Overexpression of BcERF3 increases the biosynthesis of saikosaponins in Bupleurum chinense

Han

Wan

et al. 2022

FEBS Open Bio

View full text Add to dashboard Cite

Chaihu, the dried roots of some species of Bupleurum L., is a famous Chinese herbal medicine for treatment of liver‐ and cold‐related diseases, in which saikosaponins (SSs) are the major active compounds. Many of the genetic components upstream of SS biosynthetic pathways have been characterized; however, the regulatory mechanisms remain elusive. In this study we identified the APETALA2/Ethylene Responsive Factor family transcription factor gene BcERF3 from B. chinense. The expression of BcERF3 was induced in methyl‐jasmonate‐treated adventitious root of B. chinense; it was also expressed at higher levels in roots than in other tissues (stem, leaf, flower, and tender fruit of early fruiting plants). Transient expression of BcERF3 in the leaves of Nicotiana benthamiana resulted in intracellular localization of the protein in the nucleus. It was also demonstrated that the number of SSs was greater in BcERF3‐overexpressing hairy roots of B. chinense than in plants treated with empty vector controls. This coincided with upregulation of β‐AS, which encodes a key enzyme involved with triterpenoid biosynthesis. In conclusion, BcERF3 plays a positive regulatory role in the biosynthesis of SSs.

show abstract

Functional characterization of three ethylene response factor genes from Bupleurum kaoi indicates that BkERFs mediate resistance to Botrytis cinerea

Cited by 23 publications

References 21 publications

AaERF1 Positively Regulates the Resistance to Botrytis cinerea in Artemisia annua

AaERF1 Positively Regulates the Resistance to Botrytis cinerea in Artemisia annua

Putative Genes Involved in Saikosaponin Biosynthesis in Bupleurum Species

Overexpression of BcERF3 increases the biosynthesis of saikosaponins in Bupleurum chinense

Contact Info

Product

Resources

About