Functional Characterization of the α- and β-Subunits of a Group II Chaperonin from Aeropyrum pernix K1

Abstract

We isolated and functionally characterized the α- and β- subunits (ApCpnA and ApCpnB) of a chaperonin from Aeropyrum pernix K1. The constructed vectors pET3d- ApCpnA and pET21a-ApCpnB were transformed into E. coli Rosetta (DE3), BL21 (DE3), or CodonPlus (DE3) cells. The expression of ApCpnA (60.7 kDa) and ApCpnB (61.2 kDa) was confirmed by SDS-PAGE analysis. Recombinant ApCpnA and ApCpnB were purified by heat-shock treatment and anion-exchange chromatography. ApCpnA and ApCpnB were able to hydrolyze not only ATP, but also CTP, GTP, and UTP, albeit with different efficacies. Purified ApCpnA and ApCpnB showed the highest ATPase, CTPase, UTPase, and GTPase activities at 80°C. Furthermore, the addition of ApCpnA and ApCpnB effectively protected citrate synthase (CS) and alcohol dehydrogenase (ADH) from thermal aggregation and inactivation at 43°C and 50°C, respectively. In particular, the addition of ATP or CTP to ApCpnA and ApCpnB resulted in the most effective prevention of thermal aggregation and inactivation of CS and ADH. The ATPase activity of the two chaperonin subunits was dependent on the salt concentration. Among the ions we examined, potassium ions were the most effective at enhancing the ATP hydrolysis activity of ApCpnA and ApCpnB.

Coexpression of Alginate Lyase with Hyperthermophilic Archaea Chaperonin in E. coli

“…Recombinant plasmids, pALP4, pET3d-ApCpnA, pET21a-ApCPnB, and pG-KJE6 were used in this work [11,21]. The alginate lyase gene (aly, 1.19 kb) from P. elyakovii [19] was subcloned into the pET25-vector, resulting in the plasmid pALP4 (6.7 kb) [21].…”

“…A number of biochemical studies using endogenous model substrates suggest that each subunit contributes to the recognition of specific motifs within the substrates [3,11].…”