Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure

Ceasar, S. Antony; Baker, Alison; Ignacimuthu, S.

doi:10.1038/s41598-017-14447-0

Cited by 59 publications

(26 citation statements)

References 47 publications

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“…The genotype 'Maxima' (Acc. No: Bs 3875; Welsh Plant Breeding Station, Genetic Resources Unit, Institute of Grassland and Environmental Research, Aberystwyth University, UK) previously used for genetic characterization on P response [29,35] was also included in this study. Experiments were conducted under natural field and greenhouse conditions.…”

Section: Plant Materialsmentioning

confidence: 99%

Phenotypic responses of foxtail millet (Setaria italica) genotypes to phosphate supply under greenhouse and natural field conditions

et al. 2020

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Food insecurity is a looming threat for the burgeoning world population. Phosphorus (P), which is absorbed from soil as inorganic phosphate (Pi), is an essential macronutrient for the growth of all agricultural crops. This study reports phenotype analysis for P responses in natural field and greenhouse conditions, using 54 genotypes of foxtail millet (Setaria italica) representing wide geographic origins. The genotype responses were assessed in natural field conditions in two different seasons (monsoon and summer) under Pi-fertilized (P+) and unfertilized (P-) soil for eight above-ground traits. Enormous variations were seen among the genotypes in phenotypic responses for all the measured parameters under low P stress conditions. Variations were significant for plant height, leaf number and length, tillering ability and seed yield traits. Genotypes ISe 1234 and ISe 1541 were P+ responders, and the genotypes ISe 1181, ISe 1655, ISe 783 and ISe 1892 showed tolerance to low P for total seed yield. Genotypes that performed well under P-conditions were almost as productive as genotypes that performed well under P+ conditions suggesting some genotypes are well adapted to nutrient-poor soils. In the greenhouse, most of the genotypes produced changes in root architecture that are characteristic of P-stress, but to differing degrees. Significant variation was seen in root hair density and root hair number and in fresh and dry weight of shoot and root under P-stress. However, there was not much difference in the shoot and root total P and Pi levels of five selected high and low responding genotypes. We noticed contrasting responses in the greenhouse and natural field experiments for most of these genotypes. The leads from the study form the basis for breeding and improvement of foxtail millet for better Pi-use efficiency.

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Section: Plant Materialsmentioning

confidence: 99%

Phenotypic responses of foxtail millet (Setaria italica) genotypes to phosphate supply under greenhouse and natural field conditions

et al. 2020

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“…In foxtail millet, Agrobacterium-mediated transformation was first reported by Liu et al [106]. Later studies have focused on developing transgenic foxtail millet tolerant to drought and salinity [94,97,127,181], and characterization of phosphate transport [26]. While only a few studies on the genetic transformation are available on barnyard millet, teff, and fonio and none are available on little millet, proso millet and kodo millet.…”

Section: Genetic Transformation Of Small Milletsmentioning

confidence: 99%

Genetic and genomic resources, and breeding for accelerating improvement of small millets: current status and future interventions

Vetriventhan

Azevedo

Upadhyaya

et al. 2020

Nucleus

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107

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Current agricultural and food systems encourage research and development on major crops, neglecting regionally important minor crops. Small millets include a group of small-seeded cereal crops of the grass family Poaceae. This includes finger millet, foxtail millet, proso millet, barnyard millet, kodo millet, little millet, teff, fonio, job's tears, guinea millet, and browntop millet. Small millets are an excellent choice to supplement major staple foods for crop and dietary diversity because of their diverse adaptation on marginal lands, less water requirement, lesser susceptibility to stresses, and nutritional superiority compared to major cereal staples. Growing interest among consumers about healthy diets together with climate-resilient features of small millets underline the necessity of directing more research and development towards these crops. Except for finger millet and foxtail millet, and to some extent proso millet and teff, other small millets have received minimal research attention in terms of development of genetic and genomic resources and breeding for yield enhancement. Considerable breeding efforts were made in finger millet and foxtail millet in India and China, respectively, proso millet in the United States of America, and teff in Ethiopia. So far, five genomes, namely foxtail millet, finger millet, proso millet, teff, and Japanese barnyard millet, have been sequenced, and genome of foxtail millet is the smallest (423-510 Mb) while the largest one is finger millet (1.5 Gb). Recent advances in phenotyping and genomics technologies, together with available germplasm diversity, could be utilized in small millets improvement. This review provides a comprehensive insight into the importance of small millets, the global status of their germplasm, diversity, promising germplasm resources, and breeding approaches (conventional and genomic approaches) to accelerate climate-resilient and nutrient-dense small millets for sustainable agriculture, environment, and healthy food systems.

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“…They further validated the Less Shattering1 ( SvLes1 ) gene through CRISPR/Cas9 to control seed shattering. In other studies, Agrobacterium -mediated transformation was previously performed in foxtail millet ( Setaria italica ) for downregulation of phosphate transporters (SiPHT1;2, SiPHT1;3, and SiPHT1;4) [ 237 ]. They reported significant reductions in inorganic and total phosphate in root and shoot tissues, as well as an increase in the number of roots and hairy roots for the nonredundant roles.…”

Section: Alternative Strategies For Enhancing Stress Resilience In Cropsmentioning

confidence: 99%

Alternative Strategies for Multi-Stress Tolerance and Yield Improvement in Millets

Numan

Serba

Ligaba-Osena

2021

Genes

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Millets are important cereal crops cultivated in arid and semiarid regions of the world, particularly Africa and southeast Asia. Climate change has triggered multiple abiotic stresses in plants that are the main causes of crop loss worldwide, reducing average yield for most crops by more than 50%. Although millets are tolerant to most abiotic stresses including drought and high temperatures, further improvement is needed to make them more resilient to unprecedented effects of climate change and associated environmental stresses. Incorporation of stress tolerance traits in millets will improve their productivity in marginal environments and will help in overcoming future food shortage due to climate change. Recentlly, approaches such as application of plant growth-promoting rhizobacteria (PGPRs) have been used to improve growth and development, as well as stress tolerance of crops. Moreover, with the advance of next-generation sequencing technology, genome editing, using the clustered regularly interspaced short palindromic repeats (CRISPR/Cas9) system are increasingly used to develop stress tolerant varieties in different crops. In this paper, the innate ability of millets to tolerate abiotic stresses and alternative approaches to boost stress resistance were thoroughly reviewed. Moreover, several stress-resistant genes were identified in related monocots such as rice (Oryza sativa), wheat (Triticum aestivum), and maize (Zea mays), and other related species for which orthologs in millets could be manipulated by CRISPR/Cas9 and related genome-editing techniques to improve stress resilience and productivity. These cutting-edge alternative strategies are expected to bring this group of orphan crops at the forefront of scientific research for their potential contribution to global food security.

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Functional characterization of the PHT1 family transporters of foxtail millet with development of a novel Agrobacterium-mediated transformation procedure

Cited by 59 publications

References 47 publications

Phenotypic responses of foxtail millet (Setaria italica) genotypes to phosphate supply under greenhouse and natural field conditions

Phenotypic responses of foxtail millet (Setaria italica) genotypes to phosphate supply under greenhouse and natural field conditions

Genetic and genomic resources, and breeding for accelerating improvement of small millets: current status and future interventions

Alternative Strategies for Multi-Stress Tolerance and Yield Improvement in Millets

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