Functional characterization of the endolysins derived from mycobacteriophage PDRPxv

Eniyan, Kandasamy; Sinha, Avni; Ahmad, Shazeb; Bajpai, Urmi

doi:10.1007/s11274-020-02858-7

Cited by 8 publications

(22 citation statements)

References 35 publications

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“…Mycobacteriophages encode endolysins (lytic enzymes) that can promote the lysis of phage-infected bacteria at the end of propagation [ 47 ]. Mycobacteriophages are distributed into three categories based on their capability to produce one or two endolysins, including Lysin A, Lysin A and Lysin B, and Lysin B [ 48 ]. Lysin A and Lysin B can disintegrate peptidoglycan and mycolic acid-arabinogalactan layers, respectively [ 48 ].…”

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

“…Mycobacteriophages are distributed into three categories based on their capability to produce one or two endolysins, including Lysin A, Lysin A and Lysin B, and Lysin B [ 48 ]. Lysin A and Lysin B can disintegrate peptidoglycan and mycolic acid-arabinogalactan layers, respectively [ 48 ]. All bacteriophages have lysin A domains in their genomes, whereas Lysin B is specific to some mycobacteriophages [ 49 ].…”

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

“…A study on mycobacteriophage Che12 revealed that Gp11 in this phage has Chitinase domains that function as Lysin A. Che12 lysin A cuts off NAG-NAM-NAG of the peptidoglycan structure in the mycobacterial cell wall and produces tautomers of NAG-NAM-NAG [ 50 ]. Genomes in mycobacteriophages PDRPxv and OKCentral2016 [ 51 ] can encode the two endolysins [ 48 ], whereas mycobacteriophages Ms6 [ 4 ], Donny [ 52 ], and D29 only have genes that induce the production of Lysin B [ 51 , 53 ]. Endolysin extracted from PDRPxv could affect both dividing and non-dividing host cells.…”

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

“…Endolysin extracted from PDRPxv could affect both dividing and non-dividing host cells. Mycobacterial endolysins have potential therapeutic properties against drug-resistant tuberculosis [ 48 ]. The turbidimetric and biochemical analyses revealed that PDRPxv-recombinant endolysins have antimicrobial impacts on M. smegmatis [ 48 ].…”

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

“…Mycobacterial endolysins have potential therapeutic properties against drug-resistant tuberculosis [ 48 ]. The turbidimetric and biochemical analyses revealed that PDRPxv-recombinant endolysins have antimicrobial impacts on M. smegmatis [ 48 ]. Lysin A has a secretory role and is expressed in periplasmic spaces to deteriorate its target, peptidoglycan.…”

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

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A Review on Mycobacteriophages: From Classification to Applications

Hosseiniporgham

Sechi

2022

Pathogens

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Mycobacterial infections are a group of life-threatening conditions triggered by fast- or slow-growing mycobacteria. Some mycobacteria, such as Mycobacterium tuberculosis, promote the deaths of millions of lives throughout the world annually. The control of mycobacterial infections is influenced by the challenges faced in the diagnosis of these bacteria and the capability of these pathogens to develop resistance against common antibiotics. Detection of mycobacterial infections is always demanding due to the intracellular nature of these pathogens that, along with the lipid-enriched structure of the cell wall, complicates the access to the internal contents of mycobacterial cells. Moreover, recent studies depicted that more than 20% of M. tuberculosis (Mtb) infections are multi-drug resistant (MDR), and only 50% of positive MDR-Mtb cases are responsive to standard treatments. Similarly, the susceptibility of nontuberculosis mycobacteria (NTM) to first-line tuberculosis antibiotics has also declined in recent years. Exploiting mycobacteriophages as viruses that infect mycobacteria has significantly accelerated the diagnosis and treatment of mycobacterial infections. This is because mycobacteriophages, regardless of their cycle type (temperate/lytic), can tackle barriers in the mycobacterial cell wall and make the infected bacteria replicate phage DNA along with their DNA. Although the infectivity of the majority of discovered mycobacteriophages has been evaluated in non-pathogenic M. smegmatis, more research is still ongoing to find mycobacteriophages specific to pathogenic mycobacteria, such as phage DS6A, which has been shown to be able to infect members of the M. tuberculosis complex. Accordingly, this review aimed to introduce some potential mycobacteriophages in the research, specifically those that are infective to the three troublesome mycobacteria, M. tuberculosis, M. avium subsp. paratuberculosis (MAP), and M. abscessus, highlighting their theranostic applications in medicine.

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Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

Section: An Explanation Of Discovered Mycobacteriophages Infective To...mentioning

confidence: 99%

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A Review on Mycobacteriophages: From Classification to Applications

Hosseiniporgham

Sechi

2022

Pathogens

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Insights into the genomic features and lifestyle of B1 subcluster mycobacteriophages

Das,

Arora,

Nadar

et al. 2024

J Basic Microbiol

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Bacteriophages infecting Mycobacterium smegmatis mc2155 are numerous and, hence, are classified into clusters based on nucleotide sequence similarity. Analyzing phages belonging to clusters/subclusters can help gain deeper insights into their biological features and potential therapeutic applications. In this study, for genomic characterization of B1 subcluster mycobacteriophages, a framework of online tools was developed, which enabled functional annotation of about 55% of the previously deemed hypothetical proteins in B1 phages. We also studied the phenotype, lysogeny status, and antimycobacterial activity of 10 B1 phages against biofilm and an antibiotic‐resistant M. smegmatis strain (4XR1). All 10 phages belonged to the Siphoviridae family, appeared temperate based on their spontaneous release from the putative lysogens and showed antibiofilm activity. The highest inhibitory and disruptive effects on biofilm were 64% and 46%, respectively. This systematic characterization using a combination of genomic and experimental tools is a promising approach to furthering our understanding of viral dark matter.

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