Functional characterization of the chlorophyll b reductase gene NYC1 associated with chlorophyll degradation and photosynthesis in Zoysia japonica

Teng, Ke; Tan, Penghui; Guan, Jin; Dong, D. W.; Liu, Ling-yun; Guo, Yidi; Guo, Weier; Yue, Yuesen; Fan, Xing; Wu, Juying

doi:10.1016/j.envexpbot.2021.104607

Cited by 22 publications

(22 citation statements)

References 46 publications

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“…In contrast, the other two DEGs involved in Chl degradation were downregulated in CN17 compared with CN19 at 40 DAH, while following the leaf senescence process, they were upregulated in CN19 (Table 2 and Supplementary Table 2). One of these DEGs encodes the Chl b reductase NYC1, which directly regulates Chl degradation (Teng et al, 2021), and the other encodes heme oxygenase 1, which putatively and negatively regulates Chl biosynthesis by enhancing the other branch pathways of porphyrin and inhibiting the number of porphyrins available for Chl biosynthesis (Mahawar and Shekhawat, 2018). In addition, 6 type I DEGs involved in Chl metabolism were detected, of which two (Table 2 and Supplementary Table 2) DEGs encoding protochlorophyllide reductase A, were upregulated in CN17 compared with CN19 at 20 DAH, while following leaf senescence, they were downregulated in CN17, and the expression change showed no difference in CN19.…”

Section: Identification Of Key Genes Responsible For the Functional S...mentioning

confidence: 99%

Comparative transcriptome analysis revealed differential gene expression involved in wheat leaf senescence between stay-green and non-stay-green cultivars

Yang

Guo

et al. 2022

Front. Plant Sci.

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Breeders agree that leaf senescence is a favorable process for wheat seed yield improvement due to the remobilization of leaf nutrients. However, several studies have suggested that staying green may be an important strategy for further increasing wheat yields. In this study, we performed a comparative transcriptome analysis between wheat cultivars CN17 and CN19 after heading and also measured photosynthetic parameters, chlorophyll (Chl) contents, and antioxidant enzyme activities at various time points after heading. The physiological and biochemical indexes revealed that CN17 exhibited a functionally stay-green phenotype while CN19 did not. We identified a total of 24,585 and 34,410 differential expression genes between genotypes at two time-points and between time-points in two genotypes, respectively, and we also found that 3 (37.5%) genes for leaf senescence, 46 (100%) for photosynthesis – antenna protein, 33 (70.21%) for Chl metabolism and 34 (68%) for antioxidative enzyme activity were upregulated in CN17 compared with CN19 during leaf senescence, which could be regulated by the differential expression of SAG39 (senescence-associated gene 39), while 22 (100%) genes for photosynthesis – antenna proteins, 6 (46.15%) for Chl metabolism and 12 (80%) for antioxidative enzyme activity were upregulated in CN17 compared with CN19 before the onset of leaf senescence. Here, we further clarified the expression profiles of genes associated with a functional stay-green phenotype. This information provides new insight into the mechanism underlying delayed leaf senescence and a new strategy for breeders to improve wheat yields.

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Section: Identification Of Key Genes Responsible For the Functional S...mentioning

confidence: 99%

Comparative transcriptome analysis revealed differential gene expression involved in wheat leaf senescence between stay-green and non-stay-green cultivars

Yang

Guo

et al. 2022

Front. Plant Sci.

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“…Commonly known as a warm-season turfgrass, Zoysia japonica (2n = 4x = 40) has many remarkable characteristics, including minimal maintenance, excellent tolerance to drought, salinity, and freezing, good ability to conserve water and soil, and excellent traffic tolerance (Patton and Reicher, 2007;Teng et al, 2017Teng et al, , 2018. Nevertheless, the short green period and unaesthetic appearance during senescence hamper its further popularization and utilization (Teng et al, 2016(Teng et al, , 2021b. Therefore, it is critical to interpret the molecular regulation mechanism of chlorophyll degradation and photosynthesis with the help of molecular biology.…”

Section: Introductionmentioning

confidence: 99%

Zoysia japonica Chlorophyll b Reductase Gene NOL Participates in Chlorophyll Degradation and Photosynthesis

et al. 2022

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The degradation of chlorophyll is of great significance to plant growth. The chlorophyll b reductase NOL (NYC1-like) is in charge of catalyzing the degradation of chlorophyll b and maintaining the stability of the photosystem. However, the molecular mechanisms of NOL-mediated chlorophyll degradation, senescence, and photosynthesis and its functions in other metabolic pathways remain unclear, especially in warm-season turfgrass. In this study, ZjNOL was cloned from Zoysia japonica. It is highly expressed in senescent leaves. Subcellular localization investigation showed ZjNOL is localized in the chloroplast and the bimolecular fluorescence complementation (BiFC) results proved ZjNOL interacts with ZjNYC1 in vivo. ZjNOL promoted the accumulation of abscisic acid (ABA) and carbohydrates, and the increase of SAG14 at the transcriptional level. ZjNOL simultaneously led to the excessive accumulation of reactive oxygen species (ROS), the activation of antioxidant enzymes, and the generation of oxidative stress, which in turn accelerated senescence. Chlorophyll fluorescence assay (JIP-test) analysis showed that ZjNOL inhibited photosynthetic efficiency mainly through damage to the oxygen-evolving complex. In total, these results suggest that ZjNOL promotes chlorophyll degradation and senescence and negatively affects the integrity and functionality of the photosystem. It could be a valuable candidate gene for genome editing to cultivate Z. japonica germplasm with prolonged green period and improved photosynthesis efficiency.

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“…The consistency of the biological repeats reflects the reliability of the experimental design and is the premise of follow-up analysis [10]. Sample repeatability analysis result proved the experimental design are reliable in this study.…”

Section: Transcriptional Regulating Mechanism Of Wheat Cultivars With...mentioning

confidence: 52%

“…Investigating the nitrogen metabolism at the transcriptional level helps to understand the complex response mechanisms of plants to different nitrogen levels. RNAsequencing provides an effective tool for studying biological processes, deciphering trait formation mechanisms, and interpreting gene regulatory mechanisms [10]. For instance, transcriptomic and proteomic analyses shed new light on how high nitrogen causes apple fruit quality to decline [11].…”

Section: Introductionmentioning

confidence: 99%

Transcriptional regulation mechanism of wheat varieties with different nitrogen use efficiencies in response to nitrogen deficiency stress

Wang

Fuhua

et al. 2022

BMC Genomics

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Background As one of the microelements, nitrogen play essential roles in cereal production. Although the use of chemical fertilizers has significantly improved the yield of wheat, it has also caused increasingly adverse environmental pollution. Revealing the molecular mechanism manipulating wheat nitrogen use efficiency (NUE), and cultivating wheat germplasms with high nitrogen use efficiency has become important goals for wheat researchers. In this study, we investigated the physiological and transcriptional differences of three wheat cultivars with different NUE under low nitrogen stress. Results The results showed that, under low nitrogen conditions, the activities of nitrogen metabolism-related enzymes (GS, NR, GDH), antioxidant enzymes (SOD, POD, CAT) and soluble protein contents of ZM366 (high NUE cultivar) were higher than those of JD8 (low NUE cultivar). The hybrid cultivar of ZM366 and JD8 showed mid-parent or over-parent heterosis. Transcriptome analysis revealed that ‘alanine, aspartate and glutamate metabolism’, ‘terpenoid backbone biosynthesis’ and ‘vitamin B6 metabolism’ pathways play key roles in nitrogen use efficiency in wheat. The significant enhancement of the ‘Calvin cycle’ and ‘photorespiration’ in ZM366 contributed to its higher level of carbon metabolism under low nitrogen stress, which is an important attribute differs from the other two varieties. In addition, the activation of ABA signal transduction and biosynthesis pathways also helps to maintain NUE under low- nitrogen conditions. Moreover, bHLH transcription factors were also found to play a positive role in wheat NUE. Conclusions In conclusion, these results enriched our knowledge of the mechanism of wheat NUE, and provided a theoretical basis for improving wheat NUE and breeding new cultivars.

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Functional characterization of the chlorophyll b reductase gene NYC1 associated with chlorophyll degradation and photosynthesis in Zoysia japonica

Cited by 22 publications

References 46 publications

Comparative transcriptome analysis revealed differential gene expression involved in wheat leaf senescence between stay-green and non-stay-green cultivars

Comparative transcriptome analysis revealed differential gene expression involved in wheat leaf senescence between stay-green and non-stay-green cultivars

Zoysia japonica Chlorophyll b Reductase Gene NOL Participates in Chlorophyll Degradation and Photosynthesis

Transcriptional regulation mechanism of wheat varieties with different nitrogen use efficiencies in response to nitrogen deficiency stress

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