Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H<sup>+</sup> Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport

Srivastava, Ashutosh; Ganesan, Savita; Ismail, Ihab O.; Ayre, Brian G.

doi:10.1104/pp.108.124776

Cited by 178 publications

(225 citation statements)

References 51 publications

(54 reference statements)

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“…Increased expression of SUT1 , the sucrose transporter critical for cell‐to‐cell sucrose movement and sucrose phloem loading (Srivastava et al ., 2008; Wang et al ., 2015), was also demonstrated in the roots using qRT‐PCR (Figure 11). Other genes, such as α ‐amylase , INV1 , INV2 , SUS and SUT2 , were not affected in IA lines under either condition.…”

Section: Resultsmentioning

confidence: 99%

H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

Fan

Wang

et al. 2017

Plant Biotechnology Journal

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SummaryIron (Fe) deficiency is one of the most common micronutrient deficiencies limiting crop production globally, especially in arid regions because of decreased availability of iron in alkaline soils. Sweet potato [Ipomoea batatas (L.) Lam.] grows well in arid regions and is tolerant to Fe deficiency. Here, we report that the transcription of type I H+‐pyrophosphatase (H+‐PPase) gene IbVP1 in sweet potato plants was strongly induced by Fe deficiency and auxin in hydroponics, improving Fe acquisition via increased rhizosphere acidification and auxin regulation. When overexpressed, transgenic plants show higher pyrophosphate hydrolysis and plasma membrane H+‐ATPase activity compared with the wild type, leading to increased rhizosphere acidification. The IbVP1‐overexpressing plants showed better growth, including enlarged root systems, under Fe‐sufficient or Fe‐deficient conditions. Increased ferric precipitation and ferric chelate reductase activity in the roots of transgenic lines indicate improved iron uptake, which is also confirmed by increased Fe content and up‐regulation of Fe uptake genes, e.g. FRO2,IRT1 and FIT. Carbohydrate metabolism is significantly affected in the transgenic lines, showing increased sugar and starch content associated with the increased expression of AGPase and SUT1 genes and the decrease in β‐amylase gene expression. Improved antioxidant capacities were also detected in the transgenic plants, which showed reduced H2O2 accumulation associated with up‐regulated ROS‐scavenging activity. Therefore, H+‐PPase plays a key role in the response to Fe deficiency by sweet potato and effectively improves the Fe acquisition by overexpressing IbVP1 in crops cultivated in micronutrient‐deficient soils.

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Section: Resultsmentioning

confidence: 99%

H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

Fan

Wang

et al. 2017

Plant Biotechnology Journal

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“…As expected (22,23), a fraction of the segregating progeny of heterozygous (SUC2/suc2) plants grown on sucrose-free medium in the light showed a strong retardation of root growth (Fig. 8 A and B).…”

mentioning

confidence: 91%

Photosynthetic sucrose acts as cotyledon-derived long-distance signal to control root growth during early seedling development in Arabidopsis

Kircher

Schöpfer

2012

Proc. Natl. Acad. Sci. U.S.A.

213

181

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The most hazardous span in the life of green plants is the period after germination when the developing seedling must reach the state of autotrophy before the nutrients stored in the seed are exhausted. The need for an economically optimized utilization of limited resources in this critical period is particularly obvious in species adopting the dispersal strategy of producing a large amount of tiny seeds. The model plant Arabidopsis thaliana belongs to this category. Arabidopsis seedlings promote root development only in the light. This response to light has long been recognized and recently discussed in terms of an organ-autonomous feature of photomorphogenesis directed by the red/blue light absorbing photoreceptors phytochrome and cryptochrome and mediated by hormones such as auxin and/or gibberellin. Here we show that the primary root of young Arabidopsis seedlings responds to an interorgan signal from the cotyledons and that phloem transport of photosynthesis-derived sugar into the root tip is necessary and sufficient for the regulation of root elongation growth by light.

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“…Perhaps if the apoplastic pathway is not functional, the ability to symplastically load Suc into the phloem sustains plant growth. In fact, Srivastava et al (2008) recently discussed the possibility that Arabidopsis may similarly be able to load Suc directly into the phloem using a completely symplastic pathway. Hence, it is possible that plants thought to use apoplastic phloem loading based on anatomical considerations are also conditional symplastic loaders (for discussion of mixed loading, see van Bel, 1993).…”

Section: Ricementioning

confidence: 99%

“…AtSUC2 has a biochemical affinity for Suc consistent with a role in phloem loading from the apoplast (Chandran et al, 2003). Definitive proof of a function in phloem loading came from analyses of T-DNA insertion mutations in AtSUC2 (Gottwald et al, 2000;Srivastava et al, 2008). Mutant plants have reduced Suc export from leaves, chlorotic leaves that accumulate carbohydrates, diminished shoot growth, and delayed flowering.…”

Section: Function Of Group 2 Sutsmentioning

confidence: 99%

“…Mutant plants have reduced Suc export from leaves, chlorotic leaves that accumulate carbohydrates, diminished shoot growth, and delayed flowering. Recently, using a tissue-specific promoter to complement an Atsuc2 mutant, it was shown that the only essential function in photoassimilate distribution for AtSUC2 was to load Suc into the phloem in the leaf minor veins (Srivastava et al, 2008).…”

Section: Function Of Group 2 Sutsmentioning

confidence: 99%

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Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading

2009

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Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H⁺ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport

Cited by 178 publications

References 51 publications

H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

Photosynthetic sucrose acts as cotyledon-derived long-distance signal to control root growth during early seedling development in Arabidopsis

Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading

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Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H+ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport

Cited by 178 publications

References 51 publications

H+‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

H+‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

Photosynthetic sucrose acts as cotyledon-derived long-distance signal to control root growth during early seedling development in Arabidopsis

Genetic Control of Carbon Partitioning in Grasses: Roles of Sucrose Transporters and Tie-dyed Loci in Phloem Loading

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Functional Characterization of the Arabidopsis AtSUC2 Sucrose/H⁺ Symporter by Tissue-Specific Complementation Reveals an Essential Role in Phloem Loading But Not in Long-Distance Transport

H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]

H⁺‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas (L.) Lam.]