Functional characterization of the Arabidopsis transcription factor bZIP29 reveals its role in leaf and root development

Leene, Jelle Van; Blomme, Jonas; Kulkarni, Shubhada Rajabhau; Cannoot, Bernard; Winne, Nancy De; Eeckhout, Dominique; Persiau, Geert; Slijke, Eveline Van De; Vercruysse, Leen; Bossche, Robin Vanden; Heyndrickx, Ken S.; Vanneste, Steffen; Goossens, Alain; Gevaert, Kris; Vandepoele, Klaas; González, Nathalie; Inzé, Dirk; Jaeger, Geert De

doi:10.1093/jxb/erw347

Cited by 75 publications

(82 citation statements)

References 99 publications

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“…Van Leene et al . reported that a group I bZIP transcription factor, bZIP29, interacts with regulatory B′ subunits of the protein phosphatase 2A (PP2A) and PP2A catalytic subunit. It is possible that subcellular localization of VIP1 and other group I bZIP members are regulated by PP2A complexes that are activated by mechanical and hypo‐osmotic stresses.…”

Section: Discussionmentioning

confidence: 99%

“…Mechanical or hypo-osmotic stress transiently induces dephosphorylation of VIP1, suggesting that Arabidopsis has a VIP1 phosphatase that is activated by mechanical and hypo-osmotic stresses. Van Leene et al [31] reported that a group I bZIP transcription factor, bZIP29, interacts with regulatory B 0 subunits of the protein phosphatase 2A (PP2A) and PP2A catalytic subunit. It is possible that subcellular localization of VIP1 and other group I bZIP members are regulated by PP2A complexes that are activated by mechanical and hypo-osmotic stresses.…”

Section: Discussionmentioning

confidence: 99%

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Subcellular localization of VIP1 is regulated by phosphorylation and 14‐3‐3 proteins

Takeo

Ito

2017

FEBS Letters

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Arabidopsis basic leucine zipper transcription factor VIRE2-interacting protein 1 (VIP1) changes its localization from the cytosol to the nucleus when cells are subjected to mechanical or hypo-osmotic stress, although the mechanism of this change is not known. In this study, we show that change in VIP1 subcellular localization is synchronized with a change in the VIP1 phosphorylation state that is induced by mechanical/hypo-osmotic stress. VIP1 has three phosphorylatable serine residues in HXRXXS motifs, which are 14-3-3-binding targets. Mutations of these residues results in the lack of 14-3-3 binding and prevents cytosolic localization of VIP1. These results suggest that dephosphorylation of VIP1 resulting from mechanical or hypo-osmotic stress induces nuclear localization via 14-3-3 dissociation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Subcellular localization of VIP1 is regulated by phosphorylation and 14‐3‐3 proteins

Takeo

Ito

2017

FEBS Letters

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“…Moreover, Van Leene et al. () reported that the bZIP family member, bZIP29, was an important factor in leaf development. Taken together, these findings suggest that the candidate genes may participate in the formation of LA.…”

Section: Discussionmentioning

confidence: 99%

Identification of quantitative trait loci for leaf‐related traits in an IBM Syn10 DH maize population across three environments

Guan

Zhang

et al. 2018

Plant Breeding

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Leaf‐related traits (leaf length, leaf width, leaf area and leaf angle) are very important for the yield of maize (Zea mays L) due to their influence on plant type. Therefore, it is necessary to identify quantitative trait loci (QTLs) for leaf‐related traits. In this report, 221 doubled haploid lines (DHLs) of an IBM Syn10 DH population were provided for QTL mapping. In total, 54 QTLs were detected for leaf‐related traits in single environments using a high‐density genetic linkage map. Among them, only eight common QTLs were identified across two or three environments, and the common QTLs for the four traits explained 4.38%–19.99% of the phenotypic variation. qLL‐2‐1 (bin 2.09), qLW‐2‐2 (bin 2.09), qLW‐6‐3 (bin 6.07) and qLA‐5‐2 (bin 2.09) were detected in previous studies, and qLL‐1‐1, qLAr‐1‐1, qLAr‐2‐1 and qLA‐7‐1 may be new QTLs. Notably, qLW‐6‐3 and qLA‐5‐2 were found to be major QTLs explaining 19.99% and 10.96% of the phenotypic variation, respectively. Interestingly, we found three pairs of QTLs (qLW‐2‐2 and qLAr‐2‐1, qLW‐8‐1 and qLL‐8‐2, qLL‐3‐3 and qLAr‐3‐3) that control different traits and that were located on the same chromosome or in a nearby location. Moreover, nine pairs of loci with epistatic effects were identified for the four traits. These results may provide the foundation for QTL fine mapping and for an understanding of the genetic basis of variation in leaf‐related traits.

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“…However, specific VirE2 proteins showed different, yet overlapping, specificities towards individual VIP1 homologues, potentially affecting the host range of the different bacterial species. This subset of VIP1 homologues shares somewhat redundant biological functions in uninfected cells (Tsugama et al, 2014(Tsugama et al, , 2016Van Leene et al, 2016), which suggests that this redundancy could also apply to their interactions with VirE2 during the infection process. Whereas, in nature, the host range of Agrobacterium is essentially limited to dicotyledonous plants, under laboratory conditions, many more eukaryotic species can be transformed by Agrobacterium, from monocotyledonous plants, to yeast and other fungi, to mammalian cultured cells (Lacroix et al, 2006).…”

Section: Discussionmentioning

confidence: 99%

The Agrobacterium VirE2 effector interacts with multiple members of the Arabidopsis VIP1 protein family

Wang

Guo

Citovsky

2017

Molecular Plant Pathology

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T-DNA transfer from Agrobacterium to its host plant genome relies on multiple interactions between plant proteins and bacterial effectors. One such plant protein is the Arabidopsis VirE2 interacting protein (AtVIP1), a transcription factor that binds Agrobacterium tumefaciens C58 VirE2, potentially acting as an adaptor between VirE2 and several other host factors. It remains unknown, however, whether the same VirE2 protein has evolved to interact with multiple VIP1 homologues in the same host, and whether VirE2 homologues encoded by different bacterial strains/species recognize AtVIP1 or its homologues. Here, we addressed these questions by systematic analysis (using the yeast two-hybrid and co-immunoprecipitation approaches) of interactions between VirE2 proteins encoded by four major representatives of known bacterial species/strains with functional T-DNA transfer machineries and eight VIP1 homologues from Arabidopsis and tobacco. We also analysed the determinants of the VirE2 sequence involved in these interactions. These experiments showed that the VirE2 interaction is degenerate: the same VirE2 protein has evolved to interact with multiple VIP1 homologues in the same host, and different and mutually independent VirE2 domains are involved in interactions with different VIP1 homologues. Furthermore, the VIP1 functionality related to the interaction with VirE2 is independent of its function as a transcriptional regulator. These observations suggest that the ability of VirE2 to interact with VIP1 homologues is deeply ingrained into the process of Agrobacterium infection. Indeed, mutations that abolished VirE2 interaction with AtVIP1 produced no statistically significant effects on interactions with VIP1 homologues or on the efficiency of genetic transformation.

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Functional characterization of the Arabidopsis transcription factor bZIP29 reveals its role in leaf and root development

Abstract: HighlightbZIP29, an Arabidopsis transcription factor, is expressed in proliferative tissues and involved in the regulation of cell number in the root meristem and leaves.

Cited by 75 publications

References 99 publications

Subcellular localization of VIP1 is regulated by phosphorylation and 14‐3‐3 proteins

Subcellular localization of VIP1 is regulated by phosphorylation and 14‐3‐3 proteins

Identification of quantitative trait loci for leaf‐related traits in an IBM Syn10 DH maize population across three environments

The Agrobacterium VirE2 effector interacts with multiple members of the Arabidopsis VIP1 protein family

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