Functional characterization of rat glutaryl-CoA dehydrogenase and its comparison with straight-chain acyl-CoA dehydrogenase

Wu, Long-Fei; Qiao, Yuqin; Gao, Jinbo; Deng, Guoliang; Yu, Wenhua; Chen, Gong; Li, Ding

doi:10.1016/j.bmcl.2011.09.062

Cited by 8 publications

(5 citation statements)

References 26 publications

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“…Deletion of ACADS or ACOX3 caused drop of intracellular crotonyl-CoA levels without affecting other acyl-CoAs [ 18 ]. During the amino acid metabolism of lysine, hydroxylysine and tryptophan, glutaryl-CoA dehydrogenase (GCDH) catalyzes the oxidation of glutaryl-CoA to crotonyl-CoA [ 19 , 20 ]. The GCDH deficiency caused accumulation of glutarylcarnitine and neurotoxic glutaric acid, glutaryl-CoA and 3-hydroxyglutaric acid [ 21 ].…”

Section: Regulation Mechanisms Of Kcrmentioning

confidence: 99%

“…ACADS and ACOX3 that catalyze conversion of butyryl-CoA to crotonyl-CoA during fatty acid oxidation were demonstrated to be as key crotonyl-CoA producers during endoderm differentiation [ 18 ]. In amino acid metabolism of lysine, hydroxylysine, and tryptophan, GCDH catalyzes the oxidation of glutaryl-CoA to crotonyl-CoA and CO 2 [ 19 , 20 ]. CDYL converts crotonyl-CoA into β-hydroxybutyryl-CoA [ 22 ].…”

Section: Regulation Mechanisms Of Kcrmentioning

confidence: 99%

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Protein lysine crotonylation: past, present, perspective

Jiang

Lü

et al. 2021

Cell Death Dis

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Lysine crotonylation has been discovered in histone and non-histone proteins and found to be involved in diverse diseases and biological processes, such as neuropsychiatric disease, carcinogenesis, spermatogenesis, tissue injury, and inflammation. The unique carbon–carbon π-bond structure indicates that lysine crotonylation may use distinct regulatory mechanisms from the widely studied other types of lysine acylation. In this review, we discussed the regulation of lysine crotonylation by enzymatic and non-enzymatic mechanisms, the recognition of substrate proteins, the physiological functions of lysine crotonylation and its cross-talk with other types of modification. The tools and methods for prediction and detection of lysine crotonylation were also described.

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Section: Regulation Mechanisms Of Kcrmentioning

confidence: 99%

Section: Regulation Mechanisms Of Kcrmentioning

confidence: 99%

Protein lysine crotonylation: past, present, perspective

Jiang

Lü

et al. 2021

Cell Death Dis

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“…Fluctuations in the level of intermediate metabolites such as crotonyl-CoA can affect not only the equilibrium between different metabolic pathways but also the degree and types of PTM on proteins including those important for chromatin remodeling and gene expression 27,28,35 . Crotonyl-CoA is generated from lysine/tryptophan metabolism and mitochondrial or peroxisomal fatty acid oxidation [36][37][38][39][40][41] . Addition of the crotonyl-CoA precursor crotonate to cultures can increase the cellular level of crotonyl-CoA as well as protein crotonylation 27,32 .…”

Section: Resultsmentioning

confidence: 99%

Crotonylation promotes human embryonic stem cell endodermal lineage differentiation and metabolic switch

Songyang

Zhang

Shi

et al. 2021

Preprint

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Post-translational modifications of proteins are crucial to the regulation of their activity and function. As a newly discovered acylation modification, crotonylation of non-histone proteins remains largely unexplored, particularly in human embryonic stem cells (hESCs). Here we report the investigation of induced crotonylation in hESCs, which resulted in hESCs of different pluripotency states differentiating into the endodermal lineage. We showed that increased protein crotonylation in hESCs was accompanied by transcriptomic shifts and decreased glycolysis. Through large-scale profiling of non-histone protein crotonylation, we identified metabolic enzymes as major targets of inducible crotonylation in hESCs. We further discovered GAPDH as a key glycolytic enzyme regulated by crotonylation during endodermal differentiation from hESCs, where crotonylation of GAPDH decreased its enzymatic activity thereby leading to reduced glycolysis. Our study demonstrates that crotonylation of glycolytic enzymes may be crucial to metabolic switching and cell fate determination in hESCs.

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“…These two proteins are involved in amino acid metabolism: glutamate dehydrogenase catalyses the oxidative deamination of l ‐glutamate to form α‐ketoglutarate (Mathema & Na‐Bangchang, ), whereas glutaryl‐CoA dehydrogenase is involved in oxidative decarboxylation of the glutaryl‐CoA in crotonyl‐CoA. This enzyme participates in the catabolism of some amino acids, including lysine, hydroxylysine and tryptophan (Wu et al , ).…”

Section: Discussionmentioning

confidence: 99%

Differential protein expression in the midgut of Culex quinquefasciatus mosquitoes induced by the insecticide temephos

Games

Alves

Katz

et al. 2016

Medical Vet Entomology

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Mosquitoes are vectors for pathogens of malaria, lymphatic filariasis, dengue, chikungunya, yellow fever and Japanese encephalitis. Culex quinquefasciatus Say, 1823 (Diptera: Culicidae) is a known vector of lymphatic filariasis. Its control in Brazil has been managed using the organophosphate temephos. Studies examining the proteins of Cx. quinquefasciatus that are differentially expressed in response to temephos further understanding of the modes of action of the insecticide and may potentially identify resistance factors in the mosquito. In the present study, a comparative proteomic analysis, using 2-dimensional electrophoresis coupled with matrix-assisted laser desorption/ionization (MALDI) time of flight (TOF)/TOF mass spectrometry, and bioinformatics analyses were performed to identify midgut proteins in Cx. quinquefasciatus larvae that were differentially expressed in response to exposure to temephos relative to those in untreated controls. A total of 91 protein spots were differentially expressed; 40 were upregulated and 51 were downregulated by temephos. A total of 22 proteins, predominantly upregulated, were identified as known to play a role in the immune response, whereas the downregulated proteins were involved in energy and protein catabolism. This is the first proteome study of the midgut of Cx. quinquefasciatus and it provides insights into the molecular mechanisms of insecticide-induced responses in the mosquito.

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Functional characterization of rat glutaryl-CoA dehydrogenase and its comparison with straight-chain acyl-CoA dehydrogenase

Cited by 8 publications

References 26 publications

Protein lysine crotonylation: past, present, perspective

Protein lysine crotonylation: past, present, perspective

Crotonylation promotes human embryonic stem cell endodermal lineage differentiation and metabolic switch

Differential protein expression in the midgut of Culex quinquefasciatus mosquitoes induced by the insecticide temephos

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