Functional Characterization of OXYL, A SghC1qDC LacNAc-specific Lectin from The Crinoid Feather Star Anneissia Japonica

Abstract

We identified a lectin (carbohydrate-binding protein) belonging to the complement 1q(C1q) family in the feather star Anneissia japonica (a crinoid pertaining to the phylum Echinodermata). The combination of Edman degradation and bioinformatics sequence analysis characterized the primary structure of this novel lectin, named OXYL, as a secreted 158 amino acid-long globular head (sgh)C1q domain containing (C1qDC) protein. Comparative genomics analyses revealed that OXYL pertains to a family of intronless genes found with several paralogous copies in different crinoid species. Immunohistochemistry assays identified the tissues surrounding coelomic cavities and the arms as the main sites of production of OXYL. Glycan array confirmed that this lectin could quantitatively bind to type-2 N-acetyllactosamine (LacNAc: Galβ1-4GlcNAc), but not to type-1 LacNAc (Galβ1-3GlcNAc). Although OXYL displayed agglutinating activity towards Pseudomonas aeruginosa, it had no effect on bacterial growth. On the other hand, it showed a significant anti-biofilm activity. We provide evidence that OXYL can adhere to the surface of human cancer cell lines BT-474, MCF-7, and T47D, with no cytotoxic effect. In BT-474 cells, OXYL led to a moderate activation of the p38 kinase in the MAPK signaling pathway, without affecting the activity of caspase-3. Bacterial agglutination, anti-biofilm activity, cell adhesion, and p38 activation were all suppressed by co-presence of LacNAc. This is the first report on a type-2 LacNAc-specific lectin characterized by a C1q structural fold.

Diverse Localization Patterns of an R-Type Lectin in Marine Annelids

“…Not many lectins derived from marine invertebrates were extracted from tissue homogenates, so they seem to exist apart from tissues [ 14 , 15 , 16 ]. However, these lectins are found to locate around characteristic tissues such as the guts, coelome, and spickles [ 17 , 18 , 19 ]. Together with these results, PnL provided more evidence that this Gal-binding lectin locates at the surface of various tissues of PnL in lugworm tissues and suggested binding with their endogenous ligands.…”

Is ursodeoxycholic acid therapy due to pregnant intrahepatic cholestasis change the adiponectin and fibroblast growth factor‐21 levels?—Pilot study

“…In relation to IL‐6, the duality of its effects was observed. On the one hand, it is indicated as a factor that is important in developing insulin resistance; on the other hand, it suggests that IL‐6 causes cells to become sensitive to insulin . Therefore, it seems justified to determine the changes in the expression profile of Il‐6 in patients with intrahepatic cholestasis of pregnant women treated with UDCA, which probably would indicate that the concentration of IL‐6 is dependent on adiponectin and FGF21.…”

Echinoderms Metabolites: Structure, Functions, and Biomedical Perspectives

“…For example, they contain cerebrosides [10] and gangliosides [11], which are characteristic of other deuterostomes, including Chordata, Hemichordata, and Tunicata, relatives of echinoderms [12]. Echinoderms contain lectins, glycan-specific glycoproteins with immunity functions for the producers [13], and glycosaminoglycans [14].…”