Functional characterization of MANNOSE-BINDING LECTIN 1, a G-type lectin gene family member, in response to fungal pathogens of strawberry

Abstract

Fragaria × ananassa mannose-binding lectin gene, FaMBL1, is a member of strawberry G-type lectin family involved in strawberry defense. Genome-wide identification of G-type lectin gene family was carried out in F. vesca, where 133 G-lectin genes were found. Their expression profile was retrieved from available databases showing that many G-lectin genes are actively expressed during strawberry development or interaction with pathogens. Hence, stable transgenic strawberry plants overexpressing the FaMBL1 gene were generated to better investigate the role of this gene in defense. Transformed strawberry plants were selected and evaluated for their disease-related phytohormones content and reaction to biotic stresses revealing that jasmonic acid (JA) content decreased in overexpressing lines compared to wild type (WT). Petioles of overexpressing lines inoculated with C. fioriniae had lower disease incidence than WT, and leaves of overexpressing lines challenged by B. cinerea showed remarkably smaller lesion diameters compared to WT and higher chitinase 2-1 (FaChi2-1) gene expression. Our results indicate that FaMBL1 gene plays an important role in strawberry response to fungal diseases caused by C. fioriniae and B. cinerea.

A histone deacetylase, FaSRT1‐2, plays multiple roles in regulating fruit ripening, plant growth and stresses resistance of cultivated strawberry

“…On the contrary, OE of FaSRT1-2 led to the opposite effects (Figure 6). There are also a lot of reports documenting the upregulation of the expression of anti-biotic stress genes (MBL1, PAL1, PGIP, TLP1b) and genes encoding antioxidant enzymes (SOD, CAT) in the resistance of strawberry and tomato against B. cinerea (Ma et al, 2023).…”

Plant lectins: Handymen at the cell surface

“… ( Hwang and Hwang, 2011 ) FaMBL1 Strawberry Mannose Recognition of cell wall mannose from Colletotrichum fioriniae and Botrytis cinerea. ( Ma et al, 2022 ) LORE1 Thale cress Lipopolysaccharides Biotic interaction with P. syringae . ( Luo et al, 2020 ) SD1-29 Thale cress Lipopolysaccharides Biotic interaction with P. syringae .…”

“…Only few G-type LecRLKs have been functionally characterized. CaMBL1 from pepper ( Capsicum annuum ) and FaMBL1 from strawberry ( Fragaria × ananassa ) are examples of G-type LecRLKs where the lectin domain was shown to bind cell wall mannose from Xanthomonas campestris , Colletotrichum fioriniae and Botrytis cinerea ( Hwang and Hwang, 2011 , Ma et al, 2022 ). Not all identified G-type LecRLKs make use of their carbohydrate-binding properties in the interaction with pathogenic ligands.…”

LORE receptor homomerization is required for 3‐hydroxydecanoic acid‐induced immune signaling and determines the natural variation of immunosensitivity within the Arabidopsis genus

“…Apart from SRKs, only a few SD‐RLKs have been described in more detail. They are involved in plant–pathogen interactions and symbiosis or abiotic stress responses (Walker & Zhang, 1990; Tobias et al ., 1992; Tobias & Nasrallah, 1996; Chen et al ., 2006, 2013; Kim et al ., 2009a,b; Gilardoni et al ., 2011; Cheng et al ., 2013; X. L. Sun et al ., 2013; Trontin et al ., 2014; Ranf et al ., 2015; Zou et al ., 2015; Fan et al ., 2018; Schnepf et al ., 2018; Kutschera et al ., 2019; Labbé et al ., 2019; Park et al ., 2019; Jinjun et al ., 2020; Pan et al ., 2020; Sun et al ., 2020; Liu et al ., 2021; Mondal et al ., 2021; Schellenberger et al ., 2021; Kato et al ., 2022; Ma et al ., 2022; Bao et al ., 2023; Pi et al ., 2023; Shrestha et al ., 2023; Zhou et al ., 2023). Although a growing number of members of the large SD‐RLK sub‐family are being characterized, their ligands, receptor complex formation, and downstream signaling mechanisms remain largely unknown.…”