Functional characterization of FgERG3 and FgERG5 associated with ergosterol biosynthesis, vegetative differentiation and virulence of Fusarium graminearum

Yun, Yingzi; Yin, Dafang; Dawood, Dawood H.; Liu, Xin; Chen, Yun; Ma, Zhonghua

doi:10.1016/j.fgb.2014.04.010

Cited by 31 publications

(16 citation statements)

References 58 publications

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“…[ 24 ] Ergosterol, produced by fungi, has attracted much attention due to its health properties, such as antioxidant, antiinflammatory, antihyperlipidemic and potential anticancer effects. [ 25 , 26 ] The ergosterol biosynthesis pathway is well characterized in Saccharomyces cerevisiae , while little is known about the pathway in filamentous fungi, so we investigated the genes that control sterol biosynthesis in Phellinus linteus .…”

Section: Resultsmentioning

confidence: 99%

De novo transcriptome analysis of a medicinal fungiPhellinus linteusand identification of SSR markers

Huang

Jian

et al. 2015

Biotechnology & Biotechnological Equipment

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The aim of this study was to facilitate gene discovery for functional genome studies and to identify simple sequence repeat (SSR) markers for molecular-assisted selection in Phellinus linteus. The transcriptome of Phellinus linteus was sequenced using а high-throughput RNA sequencing system – the Illumina Hiseq 2000. A total of 16,383,818 clean sequencing reads, 35,532 contigs and 25,811 unigenes were postulated. Based on similarity searches with known proteins, 19,350 genes (74.97% of the unigenes) were annotated. In the present research, 19,266, 10,978 and 7831 unigenes were mapped in Nr, Swiss-Prot and clusters of orthologous groups (COG) classifications, respectively. Of all unigenes, 6845 were categorized into three functional groups, namely biological process, cellular components and molecular function and 11,088 were annotated to 108 pathways by searching the Kyoto Encyclopedia of Genes and Genomes pathway database. A total of 1129 SSRs were identified in these unigenes. In addition, 23 candidate genes, potentially involved in sterol biosynthesis, were identified and were worthy of further investigation.

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Section: Resultsmentioning

confidence: 99%

De novo transcriptome analysis of a medicinal fungiPhellinus linteusand identification of SSR markers

Huang

Jian

et al. 2015

Biotechnology & Biotechnological Equipment

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“…The amplicons were visualized on 1.5% agarose gel. Table S3 shows the list of target genes, accession numbers, their functions, the corresponding primer pairs, and their references used to perform the RT- q PCR [ 95 , 96 , 97 , 98 , 99 , 100 , 101 , 102 ]. The efficiency of the RT- q PCR amplification (E) was determined for each primer pair as follows: Five 1:10 serial dilutions (1:1-1:10,000) were obtained for each cDNA and amplified in four replicates.…”

Section: Methodsmentioning

confidence: 99%

Chitosan Hydrochloride Decreases Fusarium graminearum Growth and Virulence and Boosts Growth, Development and Systemic Acquired Resistance in Two Durum Wheat Genotypes

et al. 2020

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Fusarium head blight (FHB) is a devastating disease for cereals. FHB is managed by fungicides at anthesis, but their efficacy is variable. Conventional fungicides accumulate in the soil and are dangerous for animal and human health. This study assayed the antifungal ability of chitosan hydrochloride against Fusarium graminearum. Chitosan reduced F. graminearum growth and downregulated the transcript of the major genes involved in the cell growth, respiration, virulence, and trichothecenes biosynthesis. Chitosan promoted the germination rate, the root and coleoptile development, and the nitrogen balance index in two durum wheat genotypes, Marco Aurelio (FHB-susceptible) and DBC480 (FHB-resistant). Chitosan reduced FHB severity when applied on spikes or on the flag leaves. FHB severity in DBC480 was of 6% at 21 dpi after chitosan treatments compared to F. graminearum inoculated control (20%). The elicitor-like property of chitosan was confirmed by the up-regulation of TaPAL, TaPR1 and TaPR2 (around 3-fold). Chitosan decreased the fungal spread and mycotoxins accumulation. This study demonstrated that the non-toxic chitosan is a powerful molecule with the potential to replace the conventional fungicides. The combination of a moderately resistant genotype (DBC480) with a sustainable compound (chitosan) will open new frontiers for the reduction of conventional compounds in agriculture.

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“…Various azoles, such as miconazole and fluconazole, besides inhibiting lanosterol 14-alpha-demethylase (erg11) of the ergosterol biosynthesis pathway, generate oxidative stress in fungi (318,319) and derepress CSP-1 in N. crassa (320). Furthermore, ergosterol is essential for spore formation in Fusarium graminearum (321). Similarly, hopanoids (bacterial steroids) are synthetized during the transition of the substrate to aerial hyphae in Streptomyces coelicolor (322).…”

Section: Transitions From Vegetative Growth To Asexual Developmentmentioning

confidence: 99%

Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures

Riquelme

Aguirre

Bartnicki-García

et al. 2018

Microbiol Mol Biol Rev

269

246

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SUMMARYFilamentous fungi constitute a large group of eukaryotic microorganisms that grow by forming simple tube-like hyphae that are capable of differentiating into more-complex morphological structures and distinct cell types. Hyphae form filamentous networks by extending at their tips while branching in subapical regions. Rapid tip elongation requires massive membrane insertion and extension of the rigid chitin-containing cell wall. This process is sustained by a continuous flow of secretory vesicles that depends on the coordinated action of the microtubule and actin cytoskeletons and the corresponding motors and associated proteins. Vesicles transport cell wall-synthesizing enzymes and accumulate in a special structure, the Spitzenkörper, before traveling further and fusing with the tip membrane. The place of vesicle fusion and growth direction are enabled and defined by the position of the Spitzenkörper, the so-called cell end markers, and other proteins involved in the exocytic process. Also important for tip extension is membrane recycling by endocytosis via early endosomes, which function as multipurpose transport vehicles for mRNA, septins, ribosomes, and peroxisomes. Cell integrity, hyphal branching, and morphogenesis are all processes that are largely dependent on vesicle and cytoskeleton dynamics. When hyphae differentiate structures for asexual or sexual reproduction or to mediate interspecies interactions, the hyphal basic cellular machinery may be reprogrammed through the synthesis of new proteins and/or the modification of protein activity. Although some transcriptional networks involved in such reprogramming of hyphae are well studied in several model filamentous fungi, clear connections between these networks and known determinants of hyphal morphogenesis are yet to be established.

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Functional characterization of FgERG3 and FgERG5 associated with ergosterol biosynthesis, vegetative differentiation and virulence of Fusarium graminearum

Cited by 31 publications

References 58 publications

De novo transcriptome analysis of a medicinal fungiPhellinus linteusand identification of SSR markers

De novo transcriptome analysis of a medicinal fungiPhellinus linteusand identification of SSR markers

Chitosan Hydrochloride Decreases Fusarium graminearum Growth and Virulence and Boosts Growth, Development and Systemic Acquired Resistance in Two Durum Wheat Genotypes

Fungal Morphogenesis, from the Polarized Growth of Hyphae to Complex Reproduction and Infection Structures

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