Functional characterization of FaNIP1;1 gene, a ripening-related and receptacle-specific aquaporin in strawberry fruit

Molina‐Hidalgo, Francisco Javier; Medina-Puche, Laura; Gelis, Samuel; Ramos, José; Sabir, Farzana; Soveral, Graça; Prista, Catarina; Iglesias‐Fernández, Raquel; Caballero, José L.; Muñoz‐Blanco, Juan; Blanco‐Portales, Rosario

doi:10.1016/j.plantsci.2015.06.013

Cited by 31 publications

(17 citation statements)

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“…This suggests that both proteins could be playing a role in water balance and/or stress. In fact, some porins implied in water balance are induced during the ripening [ 41 ]. However, the others strawberry FvNAC TFs were not clearly associated to any functional cluster, although FvNAC006 and FvNAC092 were near to the VTCW clade, suggesting a similar role for these proteins.…”

Section: Resultsmentioning

confidence: 99%

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Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits

et al. 2018

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NAC proteins are a family of transcription factors which have a variety of important regulatory roles in plants. They present a very well conserved group of NAC subdomains in the N-terminal region and a highly variable domain at the C-terminus. Currently, knowledge concerning NAC family in the strawberry plant remains very limited. In this work, we analyzed the NAC family of Fragaria vesca, and a total of 112 NAC proteins were identified after we curated the annotations from the version 4.0.a1 genome. They were placed into the ligation groups (pseudo-chromosomes) and described its physicochemical and genetic features. A microarray transcriptomic analysis showed six of them expressed during the development and ripening of the Fragaria x ananassa fruit. Their expression patterns were studied in fruit (receptacle and achenes) in different stages of development and in vegetative tissues. Also, the expression level under different hormonal treatments (auxins, ABA) and drought stress was investigated. In addition, they were clustered with other NAC transcription factor with known function related to growth and development, senescence, fruit ripening, stress response, and secondary cell wall and vascular development. Our results indicate that these six strawberry NAC proteins could play different important regulatory roles in the process of development and ripening of the fruit, providing the basis for further functional studies and the selection for NAC candidates suitable for biotechnological applications.

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Section: Resultsmentioning

confidence: 99%

“…For the drought stress treatment, we followed established protocols [ 41 ]. Fruits from green to white stages were cut along with their pedicels.…”

Section: Methodsmentioning

confidence: 99%

Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits

et al. 2018

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“…Aquaporins belong to the family of integral membrane channel proteins and play an important role in water homeostasis in plants. In strawberry fruit, it has been reported that a FaNIP1‐1 gene, positively regulated by abscisic acid and negatively regulated by auxins, is active in water transport in the fruit (Molina‐Hidalgo, et al , ). The only NIP1‐1 gene that was differentially expressed in the present study was up‐regulated at the white stage.…”

Section: Discussionmentioning

confidence: 99%

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Vallarino

Merchante

Sánchez-Sevilla

et al. 2019

Plant Biotechnology Journal

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FaMADS9 is the strawberry (Fragaria x ananassa) gene that exhibits the highest homology to the tomato (Solanum lycopersicum) RIN gene. Transgenic lines were obtained in which FaMADS9 was silenced. The fruits of these lines did not show differences in basic parameters, such as fruit firmness or colour, but exhibited lower Brix values in three of the four independent lines. The gene ontology MapMan category that was most enriched among the differentially expressed genes in the receptacles at the white stage corresponded to the regulation of transcription, including a high percentage of transcription factors and regulatory proteins associated with auxin action. In contrast, the most enriched categories at the red stage were transport, lipid metabolism and cell wall. Metabolomic analysis of the receptacles of the transformed fruits identified significant changes in the content of maltose, galactonic acid‐1,4‐lactone, proanthocyanidins and flavonols at the green/white stage, while isomaltose, anthocyanins and cuticular wax metabolism were the most affected at the red stage. Among the regulatory genes that were differentially expressed in the transgenic receptacles were several genes previously linked to flavonoid metabolism, such as MYB10, DIV, ZFN1, ZFN2, GT2, and GT5, or associated with the action of hormones, such as abscisic acid, SHP, ASR, GTE7 and SnRK2.7. The inference of a gene regulatory network, based on a dynamic Bayesian approach, among the genes differentially expressed in the transgenic receptacles at the white and red stages, identified the genes KAN1, DIV, ZFN2 and GTE7 as putative targets of FaMADS9. A MADS9‐specific CArG box was identified in the promoters of these genes.

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“…The product of the tobacco gene NtAQP1 has been shown to facilitate CO 2 membrane transport, and to contribute both to photosynthesis and stomatal movement [8,9]. The products of the strawberry AQP-encoding genes FaPIP1;1 and FaNIP1;1 both appear to be involved in the transport of water into the fruit [10,11]. A number of authors have reported that plant AQPs respond to external stress, triggering physiological adjustments which act to maintain the plant’s hydration status [12,13,14,15,16].…”

Section: Introductionmentioning

confidence: 99%

Gene-Wide Analysis of Aquaporin Gene Family in Malus domestica and Heterologous Expression of the Gene MpPIP2;1 Confers Drought and Salinity Tolerance in Arabidposis thaliana

Liu

Yang

Xin

et al. 2019

IJMS

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The aquaporins (AQPs) are a family of integral membrane proteins involved in the transcellular membrane transport of water and other small molecules. A scan of the apple (Malus domestica) genome revealed the presence of 42 genes encoding putative AQPs. Based on a phylogenetic analysis of the deduced peptide sequences of the AQPs generated by Arabidopsis thaliana, poplar (Populus trichocarpa), and rubber (Hevea brasiliensis), the apple AQPs were each assigned membership of the five established AQP subfamilies, namely the PIPs (eleven members), the TIPs (thirteen members), the NIPs (eleven members), the SIPs (five members), and the XIPs (two members). The apple AQPs included asparagine-proline-alanine (NPA) motifs, an aromatic/arginine (ar/R) selectivity filter, and the Froger’s positions. The heterologous expression of MpPIP2;1 in A. thaliana was shown to enhance the level of tolerance exhibited against both drought and salinity.

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Functional characterization of FaNIP1;1 gene, a ripening-related and receptacle-specific aquaporin in strawberry fruit

Cited by 31 publications

References 106 publications

Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits

Genome-wide analysis of the NAC transcription factor family and their expression during the development and ripening of the Fragaria × ananassa fruits

Characterizing the involvement of FaMADS9 in the regulation of strawberry fruit receptacle development

Gene-Wide Analysis of Aquaporin Gene Family in Malus domestica and Heterologous Expression of the Gene MpPIP2;1 Confers Drought and Salinity Tolerance in Arabidposis thaliana

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