Functional Characterization of Duck STING in IFN-β Induction and Anti-H9N2 Avian Influenza Viruses Infections

Cheng, Yuqiang; Shi, Shuduan; Niu, Qiaona; Zhu, Wenhan; Wang, Zhaofei; Ma, Jingjiao; Wang, Hengan; Yan, Yaxian; Sun, Jianhe

doi:10.3389/fimmu.2019.02224

Cited by 12 publications

(17 citation statements)

References 30 publications

(49 reference statements)

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“…Duck STING (DuSTING) shares 43 and 71% identity to human and chicken STING (chSTING), respectively (Cheng et al, 2019a). DuSTING is most highly expressed in the glandular stomach, followed by the trachea, lung, small intestine, spleen, kidney, and bursa (Cheng et al, 2019a). ChSTING is most highly expressed in the thymus, bursa, spleen, lung, and intestine of uninfected chickens (Ran et al, 2018).…”

Section: Stingmentioning

confidence: 99%

Pattern Recognition Receptor Signaling and Innate Responses to Influenza A Viruses in the Mallard Duck, Compared to Humans and Chickens

Campbell

Magor

2020

Front. Cell. Infect. Microbiol.

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Mallard ducks are a natural host and reservoir of avian Influenza A viruses. While most influenza strains can replicate in mallards, the virus typically does not cause substantial disease in this host. Mallards are often resistant to disease caused by highly pathogenic avian influenza viruses, while the same strains can cause severe infection in humans, chickens, and even other species of ducks, resulting in systemic spread of the virus and even death. The differences in influenza detection and antiviral effectors responsible for limiting damage in the mallards are largely unknown. Domestic mallards have an early and robust innate response to infection that seems to limit replication and clear highly pathogenic strains. The regulation and timing of the response to influenza also seems to circumvent damage done by a prolonged or dysregulated immune response. Rapid initiation of innate immune responses depends on viral recognition by pattern recognition receptors (PRRs) expressed in tissues where the virus replicates. RIG-like receptors (RLRs), Toll-like receptors (TLRs), and Nod-like receptors (NLRs) are all important influenza sensors in mammals during infection. Ducks utilize many of the same PRRs to detect influenza, namely RIG-I, TLR7, and TLR3 and their downstream adaptors. Ducks also express many of the same signal transduction proteins including TBK1, TRIF, and TRAF3. Some antiviral effectors expressed downstream of these signaling pathways inhibit influenza replication in ducks. In this review, we summarize the recent advances in our understanding of influenza recognition and response through duck PRRs and their adaptors. We compare basal tissue expression and regulation of these signaling components in birds, to better understand what contributes to influenza resistance in the duck.

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Section: Stingmentioning

confidence: 99%

Pattern Recognition Receptor Signaling and Innate Responses to Influenza A Viruses in the Mallard Duck, Compared to Humans and Chickens

Campbell

Magor

2020

Front. Cell. Infect. Microbiol.

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“…After 24 h, the lysates were immunoprecipitated with anti-HA beads, followed by immunoblotting analysis with the indicated Abs. to inhibit the replication of H9N2 AIV (22). In this study, we found that duTRIM32 directly interacts with duSTING and contributes to the production of IFN-β mediated by duSTING, though the increase level is <2-folds.…”

Section: Discussionmentioning

confidence: 64%

“…Several TRIM proteins, including TRIM29, TRIM30α, TRIM32, and TRIM56 have been reported to regulate the mammalian STING-mediated pathways (11,19,20). DuSTING was reported to activate the type I IFN pathway and exhibited antiviral effects against both DNA and RNA viruses (21,22). However, whether duTRIM32 regulates the duSTING-mediated pathway remains unknown.…”

Section: Introductionmentioning

confidence: 99%

Duck TRIM32 Functions in IFN-β Signaling Against the Infection of H5N6 Highly Pathogenic Avian Influenza Virus

Zhang

Xue

et al. 2020

Front. Immunol.

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In mammals, tripartite motif 32 (TRIM32) is essential for regulating host innate immune responses to viral infections. However, the antiviral effect of TRIM32 in birds has not been reported. Here, we cloned the full-length duck TRIM32 (duTRIM32) cDNA from total spleen RNA of Peking duck. DuTRIM32 consists of 682 amino acids and has 95.5% similarity in amino acid sequences with chicken TRIM32 and 84.9% similarity with human TRIM32, respectively. DuTRIM32 mRNA was found to be ubiquitously expressed in all tested tissues from healthy ducks. Overexpression of duTRIM32 significantly activated the IFN-β promoter and upregulated the mRNA levels of IFN-β, IRF7, and Mx, which indicates that duTRIM32 is involved in the type I IFN pathway. Furthermore, duTRIM32 was found to directly interact with duck STING (duSTING) and to contribute to the expression of IFN-β mediated by duSTING. The mRNA level of duTRIM32 was significantly upregulated in the lungs and spleens of H5N6 highly pathogenic avian influenza virus (HPAIV) infected ducks 3 days post-infection (DPI). Furthermore, overexpression of duTRIM32 could inhibit the replication of H5N6 HPAIV in duck embryo fibroblasts (DEFs). Therefore, these results indicate that duTRIM32 is involved in the type I IFN pathway and exhibit an antiviral effect against H5N6 HPAIV infection.

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“…Furthermore, STING overexpression led to significant activation of NFκB, IFN-β, and ISRE promoter elements along with an antiviral effect towards DPV infection [176]. Another study reported the antiviral effect of overexpressed STING against H9N2 LPAI virus infection in DEFs [177]. Lastly, DTMUV NS2B3 protease was demonstrated to cleave STING and abrogate downstream signaling to TBK1 signifying virus-encoded evasion mechanisms to subvert IFN induction [178].…”

Section: Stingmentioning

confidence: 96%

Avian Pattern Recognition Receptor Sensing and Signaling

Neerukonda

Katneni

2020

Veterinary Sciences

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Pattern recognition receptors (PRRs) are a class of immune sensors that play a critical role in detecting and responding to several conserved patterns of microorganisms. As such, they play a major role in the maintenance of immune homeostasis and anti-microbial defense. Fundamental knowledge pertaining to the discovery of PRR functions and their ligands continue to advance the understanding of immune system and disease resistance, which led to the rational design and/or application of various PRR ligands as vaccine adjuvants. In addition, the conserved nature of many PRRs throughout the animal kingdom has enabled the utilization of the comparative genomics approach in PRR identification and the study of evolution, structural features, and functions in many animal species including avian. In the present review, we focused on PRR sensing and signaling functions in the avian species, domestic chicken, mallard, and domestic goose. In addition to summarizing recent advances in the understanding of avian PRR functions, the present review utilized a comparative biology approach to identify additional PRRs, whose functions have been well studied in mammalians but await functional characterization in avian.

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Functional Characterization of Duck STING in IFN-β Induction and Anti-H9N2 Avian Influenza Viruses Infections

Cited by 12 publications

References 30 publications

Pattern Recognition Receptor Signaling and Innate Responses to Influenza A Viruses in the Mallard Duck, Compared to Humans and Chickens

Pattern Recognition Receptor Signaling and Innate Responses to Influenza A Viruses in the Mallard Duck, Compared to Humans and Chickens

Duck TRIM32 Functions in IFN-β Signaling Against the Infection of H5N6 Highly Pathogenic Avian Influenza Virus

Avian Pattern Recognition Receptor Sensing and Signaling

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