Functional characterization of candidate antigens of Hyalomma anatolicum and evaluation of its cross-protective efficacy against Rhipicephalus microplus

Kumar, Brijesh; Manjunathachar, H V; Nagar, Gaurav; Ravikumar, G.; Fuente, José de la; Saravanan, B. C.; Ghosh, Srikant

doi:10.1016/j.vaccine.2017.08.049

Cited by 22 publications

(33 citation statements)

References 67 publications

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“…In fact, recent results support the potential of Subolesin/Akirin as a vaccine protective antigen for the control of multiple ectoparasite vector species and transmitted pathogens (Figure 10 ). The arthropod ectoparasite species in which Subolesin/Akirin have shown protective capacity by affecting different phases of their life cycles include the genera Aedes, Anopheles, Phlebotomus, Caligus, Dermanyssus, Ornithodoros, Ixodes, Haemaphysalis, Amblyomma, Dermacentor, Hyalomma and Rhipicephalus ( Almazán et al, 2003 , 2005 , 2010 , 2012 ; de la Fuente et al, 2006a , 2010 , 2011 , 2013 ; Canales et al, 2009 ; Harrington et al, 2009 ; Zivkovic et al, 2010a ; Moreno-Cid et al, 2011 , 2013 ; Carpio et al, 2011 ; Bensaci et al, 2012 ; Merino et al, 2011a , b, 2013b ; Carreón et al, 2012 ; Manzano-Román et al, 2012 , 2015 ; de la Fuente and Kocan, 2014 ; da Costa et al, 2014 ; Shakya et al, 2014 ; Torina et al, 2014 ; Contreras et al, 2015 ; de la Fuente and Contreras, 2015 ; Lu et al, 2016 ; Olds et al, 2016 ; Contreras and de la Fuente, 2016a ; Kumar et al, 2017 ; Villar et al, 2017 ; Figure 10 ). The reduction in pathogen infection has been shown for tick-borne pathogens, Anaplasma marginale, A. phagocytophilum, Borrelia burgdorferi s.s. and Babesia bovis , and the mosquito-borne pathogen, Plasmodium berghei ( de la Fuente et al, 2011 ; Merino et al, 2011b , 2013a ; Bensaci et al, 2012 ; de la Fuente and Kocan, 2014 ; da Costa et al, 2014 ; Figure 10 ).…”

Section: Protective Capacity Of Subolesin/akirin For the Control Of Ementioning

confidence: 99%

Functional Evolution of Subolesin/Akirin

et al. 2018

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The Subolesin/Akirin constitutes a good model for the study of functional evolution because these proteins have been conserved throughout the metazoan and play a role in the regulation of different biological processes. Here, we investigated the evolutionary history of Subolesin/Akirin with recent results on their structure, protein-protein interactions and function in different species to provide insights into the functional evolution of these regulatory proteins, and their potential as vaccine antigens for the control of ectoparasite infestations and pathogen infection. The results suggest that Subolesin/Akirin evolved conserving not only its sequence and structure, but also its function and role in cell interactome and regulome in response to pathogen infection and other biological processes. This functional conservation provides a platform for further characterization of the function of these regulatory proteins, and how their evolution can meet species-specific demands. Furthermore, the conserved functional evolution of Subolesin/Akirin correlates with the protective capacity shown by these proteins in vaccine formulations for the control of different arthropod and pathogen species. These results encourage further research to characterize the structure and function of these proteins, and to develop new vaccine formulations by combining Subolesin/Akirin with interacting proteins for the control of multiple ectoparasite infestations and pathogen infection.

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Section: Protective Capacity Of Subolesin/akirin For the Control Of Ementioning

confidence: 99%

Functional Evolution of Subolesin/Akirin

et al. 2018

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“…Similarly, in FER2, only statistically significant (p < 0.01) low FCE was recorded in eggs. Unlike FER2, the FCE of TPM gene was significantly (p < 0.001) higher in fed larvae, nymphs, females, males and in the unfed adult stage (P < 0.01) ( Kumar et al., 2017 ; Manjunathachar et al., 2019 ). The functional properties of the genes were studied by RNA interference (RNAi) technology and following immunization of animals with recombinant proteins, SUB, TPM, FER2, CRT and CathL, 65.4%, 63.7%, 51.7 %, 41.3% and 30.2%, respectively, protection against larvae was observed.…”

Section: Management Of Hyalomma Speciesmentioning

confidence: 90%

“…The functional properties of the genes were studied by RNA interference (RNAi) technology and following immunization of animals with recombinant proteins, SUB, TPM, FER2, CRT and CathL, 65.4%, 63.7%, 51.7 %, 41.3% and 30.2%, respectively, protection against larvae was observed. However, against adults, the TPM and FER2 proteins were more effective ( Kumar et al., 2017 ; Manjunathachar et al., 2019 ). The results of different immunization studies clearly indicated that the majority of the experiments were conducted using the homologous challenge system and the protective efficacy of the antigens was variable.…”

Section: Management Of Hyalomma Speciesmentioning

confidence: 99%

A review on Hyalomma species infestations on human and animals and progress on management strategies

Kumar

Manjunathachar

Ghosh

2020

Heliyon

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The Hyalomma species of ticks have gained additional attention due to their role in the transmission of Theileria annulata infection in animals and the Crimean-Congo Haemorrhagic Fever (CCHF) virus in humans. Apart from these, many other pathogens viz., other species of Theileria, a few species of Babesia, Rickettsia and viruses are either maintained or transmitted by this tick species. The medium to large size species with longer proboscis has inflicted additional burden on the overall impact of tick infestations. Being a multi-host species, management of the species is very challenging. Presently, the traditional method of tick management using chemical acaricides is found insufficient and unsustainable. Henceforth, the overall burden of tick infestations and tick-borne diseases are increasing gradually. After the successful development of vaccines against cattle tick, Rhipicephalus microplus, the anti-Hyalomma vaccine is considered a feasible and sustainable management option. In the recent past research on herbal acaricides and its possible application for tick control seems promising. Other eco-friendly methods are still under experimental stage. The present review is focused on impact of Hyalomma species infestation on human and animal health with special emphasis on progress on its sustainable management.

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“…Other recently tested promising antigens include subolesin vaccine formulations including subolesin/akirin chimera which resulted in a 99% and a 46.4% vaccine efficacy in rabbits against I. ricinus and D. reticulatus , respectively (Contreras and de La Fuente, 2016 ). Additionally, recombinant subolesin tested against H. anatolicum and R. microplus infestation resulted in 65.4 and 54% protective efficacy, respectively (Kumar et al, 2017 ). The promise of this antigen is evident by the presence of a patent based on a combinatorial vaccine including Bm86 and subolesin (Patent nr.…”

Section: Ectoparasite Vaccines: Parasitic Arthropodsmentioning

confidence: 99%

Metazoan Parasite Vaccines: Present Status and Future Prospects

Stutzer

Richards

Ferreira

et al. 2018

Front. Cell. Infect. Microbiol.

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Eukaryotic parasites and pathogens continue to cause some of the most detrimental and difficult to treat diseases (or disease states) in both humans and animals, while also continuously expanding into non-endemic countries. Combined with the ever growing number of reports on drug-resistance and the lack of effective treatment programs for many metazoan diseases, the impact that these organisms will have on quality of life remain a global challenge. Vaccination as an effective prophylactic treatment has been demonstrated for well over 200 years for bacterial and viral diseases. From the earliest variolation procedures to the cutting edge technologies employed today, many protective preparations have been successfully developed for use in both medical and veterinary applications. In spite of the successes of these applications in the discovery of subunit vaccines against prokaryotic pathogens, not many targets have been successfully developed into vaccines directed against metazoan parasites. With the current increase in -omics technologies and metadata for eukaryotic parasites, target discovery for vaccine development can be expedited. However, a good understanding of the host/vector/pathogen interface is needed to understand the underlying biological, biochemical and immunological components that will confer a protective response in the host animal. Therefore, systems biology is rapidly coming of age in the pursuit of effective parasite vaccines. Despite the difficulties, a number of approaches have been developed and applied to parasitic helminths and arthropods. This review will focus on key aspects of vaccine development that require attention in the battle against these metazoan parasites, as well as successes in the field of vaccine development for helminthiases and ectoparasites. Lastly, we propose future direction of applying successes in pursuit of next generation vaccines.

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Functional characterization of candidate antigens of Hyalomma anatolicum and evaluation of its cross-protective efficacy against Rhipicephalus microplus

Cited by 22 publications

References 67 publications

Functional Evolution of Subolesin/Akirin

Functional Evolution of Subolesin/Akirin

A review on Hyalomma species infestations on human and animals and progress on management strategies

Metazoan Parasite Vaccines: Present Status and Future Prospects

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