Functional characterization of bovine von Willebrand factor gene promoter in bovine endothelial cells demonstrates species-specific properties

Janel, Nathalie; Dosne, A.M.; Obert, Bernadette; Meyer, Dominique; Kerbiriou-Nabias, Danièle

doi:10.1016/s0378-1119(97)00302-8

Cited by 12 publications

(7 citation statements)

References 21 publications

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“…The analysis of the bovine VWF promoter has also been recently reported by Janel et al (28). Sequences -113 to +229 of the bovine VWF gene were shown to function as a non-endothelial specific core promoter and sequences upstream of nucleotide -113 (-113 to -1362) were shown to function as repressor and inhibit the bovine VWF core promoter activity.…”

Section: Discussionmentioning

confidence: 54%

Two Repressor Elements Inhibit Expression of the von Willebrand Factor Gene Promoter In Vitro

Ardekani¹,

Greenberger²,

Jahroudi³

1998

Thromb Haemost

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SummaryA fragment of the human von Willebrand factor (VWF) gene promoter corresponding to sequences –487 to +247 bp functions as an endothelial specific promoter in cell culture. We have previously reported that a GATA transcription factor functions as an activator and an NF1 like protein functions as a repressor of this promoter fragment. We have now identified a second negative regulatory element in the VWF promoter that interacts with nuclear factor(s) (designated R) in both bovine aortic endothelial and smooth muscle cells. Inhibition of either the NF1 or the R repressor alone is not sufficient to activate the VWF promoter in smooth muscle cells. The present studies reveal that simultaneous inhibition of both repressors activates the VWF promoter in smooth muscle cells. The data support a model of selective derepression to explain the endothelial cell specific activity of the –487 to +247 fragment of the VWF promoter in vitro.

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Section: Discussionmentioning

confidence: 54%

Two Repressor Elements Inhibit Expression of the von Willebrand Factor Gene Promoter In Vitro

Ardekani¹,

Greenberger²,

Jahroudi³

1998

Thromb Haemost

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“…Confluent primary cells were detached by trypsin-EDTA (0.05% : 0.02%, v/v), and passages between three and five were used in the experiments. Cultures had typical cobblestone morphology and stained uniformly for human von Willebrand factor (vWF) [16] as assessed by indirect immunofluorescence.…”

Section: Methodsmentioning

confidence: 99%

Brazilin Ameliorates High Glucose-Induced Vascular Inflammation via Inhibiting ROS and CAMs Production in Human Umbilical Vein Endothelial Cells

Jayakumar

Chang

Lin

et al. 2014

BioMed Research International

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Vascular inflammatory process has been suggested to play a key role in the initiation and progression of atherosclerosis, a major complication of diabetes mellitus. Recent studies have shown that brazilin exhibits antihepatotoxic, antiplatelet, cancer preventive, or anti-inflammatory properties. Thus, we investigated whether brazilin suppresses vascular inflammatory process induced by high glucose (HG) in cultured human umbilical vein endothelial cells (HUVEC). HG induced nitrite production, lipid peroxidation, and intracellular reactive oxygen species formation in HUVEC cells, which was reversed by brazilin. Western blot analysis revealed that brazilin markedly inhibited HG-induced phosphorylation of endothelial nitric oxide synthase. Besides, we investigated the effects of brazilin on the MAPK signal transduction pathway because MAPK families are associated with vascular inflammation under stress. Brazilin blocked HG-induced phosphorylation of extracellular signal-regulated kinase and transcription factor NF-κB. Furthermore, brazilin concentration-dependently attenuated cell adhesion molecules (ICAM-1 and VCAM-1) expression induced by various concentrations of HG in HUVEC. Taken together, the present data suggested that brazilin could suppress high glucose-induced vascular inflammatory process, which may be closely related with the inhibition of oxidative stress, CAMs expression, and NF-κB activation in HUVEC. Our findings may highlight a new therapeutic intervention for the prevention of vascular diseases.

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“…For example, when reporter gene expression was analyzed in BAECs following transient transfection experiments, it was evident that the organization of the endothelium‐specific cis ‐acting regulatory elements differed between the murine [41] and human [21] Flk‐1 sequences. Furthermore, functional characterization of the bovine vWf gene promoter showed that this promoter lacked positive tissue‐specific regulatory elements between bp −1362 and + 229 [29], elements which are present in the human vWf promoter [42,43]. Thus, human promoter sequences may not always work equally well in porcine cells.…”

Section: Discussionmentioning

confidence: 99%

“…The activity and specificity of the murine and human promoters of most of these proteins have been characterized in vitro using endothelial and nonendothelial cell lines, or in vivo using transgenic mice. In most cases the activity and EC specificity have been shown to be preserved over a species barrier, although examples exist where EC‐specific promoters have been shown to have a species‐specific activity [16,29].…”

Section: Introductionmentioning

confidence: 99%

The in vitro activity and specificity of  human endothelial cell‐specific promoters  in porcine cells

He¹,

She²,

Sumitran–Holgersson³

et al. 2001

Xenotransplantation

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The chronic shortage of human organs, tissues and cells for transplantation has inspired research on the possibility of using animal donor tissue instead. Transplantation over a species barrier is associated with rejections which are difficult to control. Therefore, it is generally agreed that successful pig to human xenotransplantation requires donor pigs to be genetically modified. Vascular endothelium is the most immediate barrier between the xenogeneic donor organ and host immune and nonimmune defense systems. Thus, these cells are the prime targets for such genetic modifications. Luciferase assays were used to evaluate the activity and specificity of human endothelial-cell specific promoters in porcine aortic-, microvascular- and nonendothelial cells. The promoters for human Flk-1 (fetal liver kinase-1), Flt-1 (fms-like tyrosine kinase), ICAM-2 (intercellular adhesion molecule-2), thrombomodulin and vWf (von Willebrand factor) supported similar levels of luciferase expression in human and porcine aortic endothelial cells, with the Flk-1 promoter being the strongest followed by the thrombomodulin promoter. Relative to the activity of the CMV promoter, the human endothelial cell-specific promoters all showed less activity in porcine kidney microvascular endothelial cells than in liver or brain microvascular endothelial cells. The thrombomodulin and Flk-1 promoters exhibited similar activity in liver and kidney microvascular endothelial cells, whereas the Flk-1 promoter was stronger in aortic and brain microvascular endothelial cells. Human endothelial cell-specific promoters also showed some degree of specificity in pig, because they supported less luciferase activity in porcine nonendothelial cell lines. Based on the in vitro data and previously published in vivo data, the human Flk-1 and thrombomodulin promoters are good candidate promoters for strong endothelial cell-specific gene expression in transgenic pigs.

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Functional characterization of bovine von Willebrand factor gene promoter in bovine endothelial cells demonstrates species-specific properties

Cited by 12 publications

References 21 publications

Two Repressor Elements Inhibit Expression of the von Willebrand Factor Gene Promoter In Vitro

Two Repressor Elements Inhibit Expression of the von Willebrand Factor Gene Promoter In Vitro

Brazilin Ameliorates High Glucose-Induced Vascular Inflammation via Inhibiting ROS and CAMs Production in Human Umbilical Vein Endothelial Cells

The in vitro activity and specificity of  human endothelial cell‐specific promoters  in porcine cells

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Functional characterization of bovine von Willebrand factor gene promoter in bovine endothelial cells demonstrates species-specific properties

Cited by 12 publications

References 21 publications

Two Repressor Elements Inhibit Expression of the von Willebrand Factor Gene Promoter In Vitro

Two Repressor Elements Inhibit Expression of the von Willebrand Factor Gene Promoter In Vitro

Brazilin Ameliorates High Glucose-Induced Vascular Inflammation via Inhibiting ROS and CAMs Production in Human Umbilical Vein Endothelial Cells

The in vitro activity and specificity of human endothelial cell‐specific promoters in porcine cells

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The in vitro activity and specificity of  human endothelial cell‐specific promoters  in porcine cells