Functional characterization of a novel, highly expressed ion‐driven sugar antiporter in the thoracic muscles of <i>Helicoverpa armigera</i>

Yuan, Yiyang; Xin, Yucui; Han, Jiaguang; Zhao, Yuhan; Han, Shi-Ming; Nangong, Ziyan; Chen, Xin; Wang, Zhu-Cheng; Li, Mei; Qiu, Xinghui

doi:10.1111/1744-7917.12908

Cited by 5 publications

(10 citation statements)

References 36 publications

(69 reference statements)

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“…The supernatant was subjected to trehalose and glucose contents measurement as previously described (Yuan et al. , 2022).…”

Section: Methodsmentioning

confidence: 99%

“…One microliter sample was diluted by 10 times with distill water, and 2 μL sample was mixed with 98 μL distill water thoroughly and centrifuged at 16 000 × g for 10 min at 4 °C. The supernatant was subjected to trehalose and glucose contents measurement as previously described (Yuan et al, 2022).…”

Section: Quantification Of Whole-body Glycogen and Hemolymph Trehalos...mentioning

confidence: 99%

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Functional evaluation of the insulin/insulin‐like growth factor signaling pathway in determination of wing polyphenism in pea aphid

Yuan

Wang

et al. 2022

Insect Science

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Wing polyphenism is a common phenomenon that plays key roles in environmental adaptation of insects. Insulin/insulin‐like growth factor signaling (IIS) pathway is a highly conserved pathway in regulation of metabolism, development, and growth in metazoans. It has been reported that IIS is required for switching of wing morph in brown planthopper via regulating the development of the wing pad. However, it remains elusive whether and how IIS pathway regulates transgenerational wing dimorphism in aphid. In this study, we found that pairing and solitary treatments can induce pea aphids to produce high and low percentage winged offspring, respectively. The expression level of ILP5 (insulin‐like peptide 5) in maternal head was significantly higher upon solitary treatment in comparison with pairing, while silencing of ILP5 caused no obvious change in the winged offspring ratio. RNA interference‐mediated knockdown of FoxO (Forkhead transcription factor subgroup O) in stage 20 embryos significantly increased the winged offspring ratio. The results of pharmacological and quantitative polymerase chain reaction experiments showed that the embryonic insulin receptors may not be involved in wing polyphenism. Additionally, ILP4 and ILP11 exhibited higher expression levels in 1st wingless offspring than in winged offspring. We demonstrate that FoxO negatively regulates the wing morph development in embryos. ILPs may regulate aphid wing polyphenism in a developmental stage‐specific manner. However, the regulation may be not mediated by the canonical IIS pathway. The findings advance our understanding of IIS pathway in insect transgenerational wing polyphenism.

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“…The supernatant was subjected to trehalose and glucose contents measurement as previously described (Yuan et al. , 2022).…”

Section: Methodsmentioning

confidence: 99%

Section: Quantification Of Whole-body Glycogen and Hemolymph Trehalos...mentioning

confidence: 99%

Functional evaluation of the insulin/insulin‐like growth factor signaling pathway in determination of wing polyphenism in pea aphid

Yuan

Wang

et al. 2022

Insect Science

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“…To the best of our knowledge, there are no reports that the SLC5 family is involved in the transport of trehalose. To date in insects, besides TRET1, three other proteins, HaST10, Pippin and MFS3, have been reported to transport trehalose (24, 25, 44-47). TRET1, HaST10 and Pippin are all facilitative diffusion-type passive transporters belonging to the SLC2 family (24, 25, 44-47).…”

Section: Introductionmentioning

confidence: 99%

“…To date in insects, besides TRET1, three other proteins, HaST10, Pippin and MFS3, have been reported to transport trehalose (24, 25, 44-47). TRET1, HaST10 and Pippin are all facilitative diffusion-type passive transporters belonging to the SLC2 family (24, 25, 44-47). MFS3 belonging to the SLC17 family transports trehalose and glucose (46).…”

Section: Introductionmentioning

confidence: 99%

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A Sodium-dependent Trehalose Transporter Contributes to Anhydrobiosis in Insect Cell Line, Pv11

Mizutani,

Yoshida,

Nakanishi

et al. 2023

Preprint

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Pv11 is the only animal cell culture that, when preconditioned with a high concentration of trehalose, can be preserved in the dry state at room temperature for more than one year while retaining the ability to resume proliferation. This extreme desiccation tolerance is referred to as anhydrobiosis. Here we identified a novel transporter that contributes to the recovery of Pv11 cells from anhydrobiosis. In general, the SLC5 family of secondary active transporters co-transport Na+and carbohydrates including glucose. Here we identified a novel transporter STRT1 (sodium-ion trehalose transporter 1) belonging to the SLC5 family that is highly expressed in Pv11 cells and transports trehalose with Na+dependency. This is the first report of an SLC5 family member that transports a naturally occurring disaccharide, such as trehalose. Knockout of theStrt1gene significantly reduced the viability of Pv11 cells upon rehydration after desiccation. During rehydration, when intracellular trehalose is no longer needed,Strt1-knockout cells released the disaccharide more slowly than the parent cell line. During rehydration, Pv11 cells became roughly spherical due to osmotic pressure changes, but then returned to their original spindle shape after about 30 min.Strt1-knockout cells, however, required about 50 min to adopt their normal morphology. STRT1 probably regulates intracellular osmolality by releasing unwanted intracellular trehalose with Na+, thereby facilitating the recovery of normal cell morphology during rehydration. STRT1 likely improves the viability of dried Pv11 cells by rapidly alleviating the significant physical stresses that arise during rehydration.Significance StatementThis is the first report of an SLC5 family member, STRT1 (sodium ion trehalose transporter 1), with Na+-dependent trehalose transport activity. AStrt1-knockout cell line revealed that STRT1 likely plays an important role during anhydrobiosis in Pv11 cells: it efficiently discharges unwanted trehalose in the presence of Na+during rehydration of dried Pv11 cells, effectively reducing intracellular osmolality and thereby restoring cell morphology to a normal state.

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Ecology and Evolution of Secondary Compound Detoxification Systems in Caterpillars

Groen

Whiteman

2022

Fascinating Life Sciences

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Functional characterization of a novel, highly expressed ion‐driven sugar antiporter in the thoracic muscles of Helicoverpa armigera

Cited by 5 publications

References 36 publications

Functional evaluation of the insulin/insulin‐like growth factor signaling pathway in determination of wing polyphenism in pea aphid

Functional evaluation of the insulin/insulin‐like growth factor signaling pathway in determination of wing polyphenism in pea aphid

A Sodium-dependent Trehalose Transporter Contributes to Anhydrobiosis in Insect Cell Line, Pv11

Ecology and Evolution of Secondary Compound Detoxification Systems in Caterpillars

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