Functional Characterization of a New Cold-Adapted β-Galactosidase from an Arctic Fjord Sediment Bacteria Enterobacter ludwigii MCC 3423

Alikunju, Aneesa P.; Joy, Susan; Salam, Jaseetha Abdul; Silvester, Reshma; Antony, Ally C.; Rahiman, K M Mujeeb; Krishnan, K. P.; Hatha, A. A. Mohamed

doi:10.1007/s10562-018-2504-3

Cited by 9 publications

(6 citation statements)

References 49 publications

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“…[ 22 ], Rahnella aquatilis from Xin-Jiang, China soil [ 32 ], Planoccocus sp. [ 33 ] from Daqing oil field in China and Enterobacter ludwigii from an Arctic Fjord [ 19 ]). Other few enzymes showed higher efficiencies in cold, among them, Rahnella sp.…”

Section: Resultsmentioning

confidence: 99%

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Antarctic Rahnella inusitata: A Producer of Cold-Stable β-Galactosidase Enzymes

Núñez-Montero

Salazar

Santos

et al. 2021

IJMS

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There has been a recent increase in the exploration of cold-active β-galactosidases, as it offers new alternatives for the dairy industry, mainly in response to the current needs of lactose-intolerant consumers. Since extremophilic microbial compounds might have unique physical and chemical properties, this research aimed to study the capacity of Antarctic bacterial strains to produce cold-active β-galactosidases. A screening revealed 81 out of 304 strains with β-galactosidase activity. The strain Se8.10.12 showed the highest enzymatic activity. Morphological, biochemical, and molecular characterization based on whole-genome sequencing confirmed it as the first Rahnella inusitata isolate from the Antarctic, which retained 41–62% of its β-galactosidase activity in the cold (4 °C–15 °C). Three β-galactosidases genes were found in the R. inusitata genome, which belong to the glycoside hydrolase families GH2 (LacZ and EbgA) and GH42 (BglY). Based on molecular docking, some of these enzymes exhibited higher lactose predicted affinity than the commercial control enzyme from Aspergillus oryzae. Hence, this work reports a new Rahnella inusitata strain from the Antarctic continent as a prominent cold-active β-galactosidase producer.

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Section: Resultsmentioning

confidence: 99%

“…[ 17 ], Paracoccus sp. [ 18 ], Enterobacter ludwigii [ 19 ], Halomonas sp. [ 20 ], Alkalilactibacillus ikkense [ 21 ], and Marinomonas sp.…”

Section: Introductionmentioning

confidence: 99%

Antarctic Rahnella inusitata: A Producer of Cold-Stable β-Galactosidase Enzymes

Núñez-Montero

Salazar

Santos

et al. 2021

IJMS

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“…Moreover, based on the thermal and pH tolerance, β‐Galactosidases can be characterised as thermophilic (Murphy and Walsh 2019), psychrophilic (Alikunju et al . 2018), thermo‐acidophilic (Alikunju et al . 2018) and thermo‐alkaliphilic (Maruthamuthu and van Elsas 2017).…”

Section: Characterisation Of β‐Galactosidasesmentioning

confidence: 99%

β‐Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine

Zhou,

Liu,

Gao

et al. 2024

Int J of Dairy Tech

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β‐Galactosidases are crucial enzymes that hydrolyse oligosaccharides and polysaccharides with terminal β‐1,4‐glycosidic bonds. Though the traditional application of β‐Galactosidases has been to catalyse the breakdown of lactose in dairy products, its application extends beyond the production of lactose‐free products since variants capable of facilitating lactose condensation and exhibiting galactosyl transferase activity are extensively utilised for the synthesis of prebiotic galacto‐oligosaccharides. This review analyses β‐Galactosidase in multiple aspects, including sources, classification, characterisation, immobilisation, genetic engineering and applications in terms of whey treatment, biofuel production, production of lactose‐free dietary product, synthesis of galacto‐oligosaccharides and the early detection of cellular senescence and tumours.

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“…Cold-active endoglucanase (CelX) from psychrotrophic Pseudoalteromonas sp. DY3 and β-galactosidase enzyme produced by Enterobacter ludwigii are regarded as possible and economically attractive alternatives to hydrolyze lignocellulose at refrigerated temperatures (Zeng et al, 2006;Alikkunju et al, 2016;Alikunju et al, 2018). These psychrophilic endocellulases can also be used as detergents in laundry as well as to produce biofuels and chemicals (Yuan et al, 2018).…”

Section: Psychrophiles and Psychrotolerant Bacteriamentioning

confidence: 99%

Recent Development of Extremophilic Bacteria and Their Application in Biorefinery

Zhu

Adebisi

Ahmad

et al. 2020

Front. Bioeng. Biotechnol.

105

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The biorefining technology for biofuels and chemicals from lignocellulosic biomass has made great progress in the world. However, mobilization of laboratory research toward industrial setup needs to meet a series of criteria, including the selection of appropriate pretreatment technology, breakthrough in enzyme screening, pathway optimization, and production technology, etc. Extremophiles play an important role in biorefinery by providing novel metabolic pathways and catalytically stable/robust enzymes that are able to act as biocatalysts under harsh industrial conditions on their own. This review summarizes the potential application of thermophilic, psychrophilic alkaliphilic, acidophilic, and halophilic bacteria and extremozymes in the pretreatment, saccharification, fermentation, and lignin valorization process. Besides, the latest studies on the engineering bacteria of extremophiles using metabolic engineering and synthetic biology technologies for high-efficiency biofuel production are also introduced. Furthermore, this review explores the comprehensive application potential of extremophiles and extremozymes in biorefinery, which is partly due to their specificity and efficiency, and points out the necessity of accelerating the commercialization of extremozymes.

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Functional Characterization of a New Cold-Adapted β-Galactosidase from an Arctic Fjord Sediment Bacteria Enterobacter ludwigii MCC 3423

Cited by 9 publications

References 49 publications

Antarctic Rahnella inusitata: A Producer of Cold-Stable β-Galactosidase Enzymes

Antarctic Rahnella inusitata: A Producer of Cold-Stable β-Galactosidase Enzymes

β‐Galactosidase: Insights into source variability, genetic engineering, immobilisation and diverse applications in food, industry and medicine

Recent Development of Extremophilic Bacteria and Their Application in Biorefinery

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