Functional characterization of a cold related flavanone 3-hydroxylase from Tetrastigma hemsleyanum: an in vitro, in silico and in vivo study

Wu, Lishuang; Tian, Jian; Yu, Yao; Yuan, Lixia; Zhang, Yujiong; Wu, Hao; Wang, Furong; Peng, Xin

doi:10.1007/s10529-023-03440-5

Cited by 2 publications

(1 citation statement)

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“…Next, to test whether the DEGs are closely related to cold response, we checked their possible gene functions through gene function annotation and literature mining. Indeed, we observed that many of them are directly involved in plant cold response or cold acclimation process, including genes encoding superoxide dismutase (SOD) (Lin et al, 2019), VERNALIZATION INSENSITIVE 3(VIN3) (Zhao et al, 2020), transcription factor LUX (Chow et al, 2014), RNA-binding protein CP29B (Kupsch et al, 2012), flavanone 3-dioxygenase 3 (Wu et al, 2023), and dehydration-responsive element-binding protein 2E (DREB2E) (Agarwal et al, 2017), etc. Also, many genes annotated as cold response or cold induced genes are observed among these DEGs (Table S6 and S7).…”

Section: Gene Expression Of Cold-stress Regulated Genes In Nts57mentioning

confidence: 99%

Genome assembly and genomic architecture of a prominent cold-resistant rapeseed germplasm

Wu,

Zheng,

Sun

et al. 2023

Preprint

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Cold stress is one of the most limiting factors affecting crop production. Winter rapeseed, as an important oilseed crop, is often subjected to low temperature stress and consequent yield loss at high latitudes. Therefore, identification of highly cold-resistant winter type rapeseed and elucidation of the molecular mechanism of cold tolerance are crucial for genetic improvement of the trait. Here, we report a chromosome-level reference genome of a prominent cold-resistant winter rapeseed cultivar, NTS57. We elucidated the phylogeny of NTS57 with other public rapeseed cultivars and performed gene family and structure variation analysis at the pan-genome level. In addition, we profiled the DNA methylation and transcriptomic changes of NTS57 upon cold stress, and many cold response genes were identified. Furthermore, we surveyed the cold resistance of 290 rapeseed accessions and performed GWAS analysis to identify the potential gene loci controlling cold resistance, which discovered some important functional genes, includingMYB34andRLK1.Overall, this research provided a basis for genetic dissection and improvement of cold tolerance in rapeseed.

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