2011
DOI: 10.1186/1754-6834-4-50
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Functional characterization and target discovery of glycoside hydrolases from the digestome of the lower termite Coptotermes gestroi

Abstract: BackgroundLignocellulosic materials have been moved towards the forefront of the biofuel industry as a sustainable resource. However, saccharification and the production of bioproducts derived from plant cell wall biomass are complex and lengthy processes. The understanding of termite gut biology and feeding strategies may improve the current state of biomass conversion technology and bioproduct production.ResultsThe study herein shows comprehensive functional characterization of crude body extracts from Copto… Show more

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Cited by 38 publications
(30 citation statements)
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“…The kinetic parameters were calculated from initial velocities using p-NPG at 0.5-14 mM concentrations. Reactions were carried out in 50 mM sodium acetate buffer (pH 5.5) with 0.5 μg purified BGs at 40°C and was stopped by adding 1 M Na 2 CO 3 [24]. The rate of pnitrophenol (p-NP) released was measured every 1 min by absorbance at 410 nm.…”
Section: Kinetic Parameter Determination and Mutant Activity Assaymentioning
confidence: 99%
“…The kinetic parameters were calculated from initial velocities using p-NPG at 0.5-14 mM concentrations. Reactions were carried out in 50 mM sodium acetate buffer (pH 5.5) with 0.5 μg purified BGs at 40°C and was stopped by adding 1 M Na 2 CO 3 [24]. The rate of pnitrophenol (p-NP) released was measured every 1 min by absorbance at 410 nm.…”
Section: Kinetic Parameter Determination and Mutant Activity Assaymentioning
confidence: 99%
“…In the termite gut, carbohydrate-active enzymes (CAZymes), such as cellulases and hemicellulases, are secreted (i.e., both symbiotic and endogenous enzymes). Additionally, a set of pro-oxidant, antioxidant, and detoxification enzymes (PADs) is also present [57]. Among the PADs found in termites, superoxide dismutase (SOD), catalases (CATs), glutathione S-transferase (GST), and aldo–keto reductases (AKRs) have been studied in detail because the transcription of mRNAs encoding these enzymes is upregulated in response to lignocellulose degradation [8].…”
Section: Introductionmentioning
confidence: 99%
“…Biomass-degrading microorganisms also often act in synergy in order to optimize their growth efforts by minimizing the energetic expense for production of the required degradation enzymes and by maximizing benefits of their common action. For these reasons, metagenomic studies focusing on whole microbial communities rather than isolated organisms, are more likely to identify interesting enzymes for lignocellulose degradation, especially when these communities are derived from specialized communities from lignocellulose-rich ecological niches, such as those from soil, or from specific natural or artificial environments such as compost, or the digestive tract of large herbivores or termites [47]. …”
Section: Introductionmentioning
confidence: 99%