Functional characteristics of New Zealand wheat rhizosphere &lt;i&gt;Pseudomonas fluorescens&lt;/i&gt; isolates and their potential to inhibit invitro growth of &lt;i&gt;Gaeumannomyces graminis&lt;/i&gt; var &lt;i&gt;tritici&lt;/i&gt;

Warren, Rachael; Chng, Soonie; Butler, R.C.

doi:10.30843/nzpp.2016.69.5914

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…Goldfarb et al ( 2011 ) showed that Buttiauxella warmboldiae str. DSM 9404 preferentially used the amino acid glycine as a labile carbon source for growth in soil microcosms and glycine is exuded by healthy wheat roots (Warren et al, 2016 ). The substantial difference in expression of genes of Buttiauxella spp between suppressive and non-suppressive rhizosphere soil requires further investigation.…”

Section: Discussionmentioning

confidence: 99%

Comparative Metatranscriptomics of Wheat Rhizosphere Microbiomes in Disease Suppressive and Non-suppressive Soils for Rhizoctonia solani AG8

et al. 2018

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The soilborne fungus Rhizoctonia solani anastomosis group (AG) 8 is a major pathogen of grain crops resulting in substantial production losses. In the absence of resistant cultivars of wheat or barley, a sustainable and enduring method for disease control may lie in the enhancement of biological disease suppression. Evidence of effective biological control of R. solani AG8 through disease suppression has been well documented at our study site in Avon, South Australia. A comparative metatranscriptomic approach was applied to assess the taxonomic and functional characteristics of the rhizosphere microbiome of wheat plants grown in adjacent fields which are suppressive and non-suppressive to the plant pathogen R. solani AG8. Analysis of 12 rhizosphere metatranscriptomes (six per field) was undertaken using two bioinformatic approaches involving unassembled and assembled reads. Differential expression analysis showed the dominant taxa in the rhizosphere based on mRNA annotation were Arthrobacter spp. and Pseudomonas spp. for non-suppressive samples and Stenotrophomonas spp. and Buttiauxella spp. for the suppressive samples. The assembled metatranscriptome analysis identified more differentially expressed genes than the unassembled analysis in the comparison of suppressive and non-suppressive samples. Suppressive samples showed greater expression of a polyketide cyclase, a terpenoid biosynthesis backbone gene (dxs) and many cold shock proteins (csp). Non-suppressive samples were characterised by greater expression of antibiotic genes such as non-heme chloroperoxidase (cpo) which is involved in pyrrolnitrin synthesis, and phenazine biosynthesis family protein F (phzF) and its transcriptional activator protein (phzR). A large number of genes involved in detoxifying reactive oxygen species (ROS) and superoxide radicals (sod, cat, ahp, bcp, gpx1, trx) were also expressed in the non-suppressive rhizosphere samples most likely in response to the infection of wheat roots by R. solani AG8. Together these results provide new insight into microbial gene expression in the rhizosphere of wheat in soils suppressive and non-suppressive to R. solani AG8. The approach taken and the genes involved in these functions provide direction for future studies to determine more precisely the molecular interplay of plant-microbe-pathogen interactions with the ultimate goal of the development of management options that promote beneficial rhizosphere microflora to reduce R. solani AG8 infection of crops.

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Section: Discussionmentioning

confidence: 99%

Comparative Metatranscriptomics of Wheat Rhizosphere Microbiomes in Disease Suppressive and Non-suppressive Soils for Rhizoctonia solani AG8

et al. 2018

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“…The commonly produced antibiotics by PGPR that act on biocontrol mechanisms are phenazine, 2,4-diacetylphloroglucinol (DAPG), pyrrolnitrin, surfactin, iturin, fengycin, viscosinamide, kanosamine, zwittermicin-A, polymyxin, circulin, pantocin, subtilin, and subtilosin (Kenawy et al, 2019). The antifungal antibiotic phenazine, produced by Pseudomonads, possesses redox activity and can suppress fungal pathogens of plants such as Fusarium oxysporum and Gaeumannomyces graminis (Mazurier et al, 2009;Warren et al, 2016). An effective and extensively studied antibiotic DAPG produced by Pseudomonas fluorescens has been reported for the destruction of the fungal cell membrane of F. oxysporum sp.…”

Section: Antifungal Antibioticsmentioning

confidence: 99%

Anti-fungal secondary metabolites and hydrolytic enzymes from rhizospheric bacteria in crop protection: a review

Chowdhury¹,

Zaman²,

Islam³

et al. 2021

J Bangladesh Acad Sci

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Plant growth promoting rhizobacteria (PGPR) residing in soil rhizosphere provide enormous beneficial effects to a plant host producing diverse secondary metabolites and enzymes useful for plant growth and protection. Siderophores, antibiotics, volatile compounds and hydrolytic enzymes are the major molecules secreted by the PGPRs, which have substantial antifungal properties and can provide plant protection. These compounds are responsible for the lysis and hyperparasitism of antagonists against deleterious fungal pathogens. Siderophore-producing PGPRs function by depriving the pathogen of iron nutrition. Antibiotics have been reported to be involved in the suppression of different fungal pathogens by inducing fungistasis, inhibition of spore germination, lysis of fungal mycelia. The PGPRs also secrete a wide range of low molecular weight volatile organic compounds (VOCs) that inhibit mycelial growth, sporulation, germination of phytophathogenic fungi, etc. Hydrolytic enzymes, mostly chitinase, protease and cellulose, lyse the cell wall of fungi. Therefore, plant growth-promoting rhizobacteria can be considered as an effective, eco-friendly, and sustainable replacement to the chemical fungicides. There are many PGPRs that perform very well in controlled conditions but not in field conditions, and hence the commercializing of hese products is not easy. Development of formulations with increased shelf life, a broad spectrum of action and consistent performance under field conditions can pave the way for commercializing the PGPRs at a faster rate. Journal of Bangladesh Academy of Sciences, Vol. 44, No. 2, 69-84, 2020

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“…Couch ( 8 (0.8,55.3) The correlation (Pearson's r) between take-all and log10 Ggt DNA concentrations in wheat roots (r=0.46) and couch rhizomes (r=0.47) were weak. discovered by Warren et al (2016) that the PF1208-4 isolate of P. fluorescens produced siderophores only, and not hydrogen cyanide produced by other P. fluorescens isolates collected from roots of second-year wheat in New Zealand. Also, the PF1208-4 isolate does not contain the PhlD gene, which codes for production of the antibiotic 2,4-diacetylphloroglucinol effective in suppressing take-all in some countries (Weller et al 2007).…”

Section: Treatmentmentioning

confidence: 99%

Influence of glyphosate herbicide treatment of couch grass on take-all caused by <i>Gaeumannomyces graminis</i> var. <i>tritici</i> with the addition of soil-borne microorganisms

Toor

Chng

Warren

et al. 2017

NZPP

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Couch grass rhizomes harbour Gaeumannomyces graminis var. tritici (Ggt), which causes take-all of wheat. Glyphosate used after cereal harvest to control couch can increase take- all in subsequent wheat crops. Following glyphosate treatment, the colonisation of senescing couch rhizomes by Ggt when treated with the endophytic fungus Microdochium bolleyi, and the spread of Ggt from senescing couch rhizomes to wheat when treated with the rhizobacterium Pseudomonas fluorescens, were investigated in two separate experiments. In Experiment 1, glyphosate increased Ggt inoculum in couch, irrespective of whether M. bolleyi was added to the potting medium. In Experiment 2, take-all severity and Ggt DNA concentration in roots of the accompanying wheat plants tended to decrease with glyphosate treatment of couch and increase only when P. fluorescens was added. Soil-borne microflora in fields containing glyphosate-sprayed couch may affect expression of take-all in subsequent wheat.

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Functional characteristics of New Zealand wheat rhizosphere <i>Pseudomonas fluorescens</i> isolates and their potential to inhibit invitro growth of <i>Gaeumannomyces graminis</i> var <i>tritici</i>

Cited by 4 publications

References 12 publications

Comparative Metatranscriptomics of Wheat Rhizosphere Microbiomes in Disease Suppressive and Non-suppressive Soils for Rhizoctonia solani AG8

Comparative Metatranscriptomics of Wheat Rhizosphere Microbiomes in Disease Suppressive and Non-suppressive Soils for Rhizoctonia solani AG8

Anti-fungal secondary metabolites and hydrolytic enzymes from rhizospheric bacteria in crop protection: a review

Influence of glyphosate herbicide treatment of couch grass on take-all caused by <i>Gaeumannomyces graminis</i> var. <i>tritici</i> with the addition of soil-borne microorganisms

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