Functional characteristics of neutrophils collected and stored after administration of G–CSF

Abstract

Neutrophils collected after the administration of G-CSF retained functional and biochemical characteristics for at least 24 hours of storage, which suggests additional effects of G-CSF mobilization beyond enhancing PMN yields and the possibility of storage of these components after collection.

Anoxia and glucose supplementation preserve neutrophil viability and function

“…G-CSF was previously shown to prolong neutrophil survival, 17,18 in particular by inhibiting the increase in intracellular calcium levels associated with apoptosis. 19,20 It was also described that G-CSF induced pseudo-Pelger-Huët anomalies, similar to the ones observed in hereditary neutrophils morphological modifications. 21 Consistently, symmetric bilobed nuclei in neutrophils purified from G-CSF-stimulated volunteers were observed ( Figure 1B), which is a typical pseudo-Pelger-Huët anomaly.…”

Granulocyte transfusions in the G-CSF era. Where do we stand?

“…25,26 A total dose of G-CSF of 300À450 mg given subcutaneously produces maximal neutrophil increments, and further escalation of the dose does not produce additional increments. 22,27 Importantly, G-CSF mobilised neutrophils have apparently normal function when tested in assays of respiratory burst chemiluminescence, 25,28,29 phagocytosis, 28 chemotaxis 28,30 and superoxide anion production. 28,30 When G-CSF is combined with glucocorticoid administration to the donor the peripheral blood neutrophil count may be increased further 22,31 and the neutrophil function maintained.…”

“…Recent studies have suggested that G-CSF mobilised granulocytes may maintain functional viability and normal chemotactic responses for up to 24 h in vitro. 30 By reducing the temperature of the stored granulocytes viability may be maintained further. 40 However, the optimal conditions to maintain granulocyte viability in vitro require further study and currently storage of GTX for patients beyond 24 h cannot be recommended.…”

Granulocyte concentrates: prolonged functional capacity during storage in the presence of phenotypic changes

“…In our study, mobilized neutrophils from granulocyte concentrates retained the ability to undergo chemotaxis in response to a panel of chemoattractants (IL-8, C5a, PAF) after storage at ambient temperature, which is in agreement with the observations of Leavey et al and those of Hubel et al on the effects of storage at room temperature or 10°C, respectively, for 24h. 38,39,43 Another important issue is whether stored granulocytes retain antifungal activity. Individuals with severely prolonged neutropenia or neutrophil dysfunction have an increased risk of developing fungal infections.…”