Functional association between NUCKS1 gene and Parkinson disease: A potential susceptibility biomarker

Singh, Sukhchain; Seth, Prahlad K.

doi:10.6026/97320630015548

Cited by 12 publications

(8 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Its expression is downregulated in PD patients, but its causal mechanisms are unknown. 58 Table of top-ranking SNPs (based on GWAS p value) in open chromatin at active regulatory regions of DNA in microglia. See also Table S1 and Figure S2 .…”

Section: Resultsmentioning

confidence: 99%

Parkinson’s disease risk enhancers in microglia

Booms,

Pierce,

van der Schans

et al. 2024

iScience

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

Parkinson’s disease risk enhancers in microglia

Booms,

Pierce,

van der Schans

et al. 2024

iScience

View full text Add to dashboard Cite

“…NUCKS1 is highly expressed in various tumors [5,6] . To investigate the expression of NUCKS1 in endometriosis, which has similar characteristics with tumor, we collected ectopic endometrium and eutopic endometrium from patients with endometriosis, along with normal endometrial tissues.…”

Section: Discussionmentioning

confidence: 99%

“…The Nuclear Ubiquitous Casein and cyclin-dependent Kinase Substrate 1(NUCKS1)gene is located on human chromosome 1q32.1 [3] and encodes the NUCKS1 protein, a 27 kDa nuclear DNA binding protein that plays an important role in cell growth and proliferation [4] . NUCKS1 plays an important role in nonneoplastic diseases such as Parkinson disease [5] and bipolar disorder [6] . Moreover, NUCKS1 is highly expressed in a variety of malignancies and acts as a tumor-associated protein regulating cellular transcription and repair [7,8] .…”

Section: Introductionmentioning

confidence: 99%

NUCKS1 is Overexpressed in Endometriosis

Li¹,

Chen

Wang³

et al. 2023

Preprint

View full text Add to dashboard Cite

Background Endometriosis is still a difficult problem for women.The Nuclear Ubiquitous Casein and cyclin-dependent Kinase Substrate 1(NUCKS1)gene is located on human chromosome 1q32.1. It encodes the NUCKS1 protein, a 27 kDa nuclear DNA binding protein that plays an important role in cell growth and proliferation. NUCKS1 plays an important role in the development of many diseases. However, its role in endometriosis is unclear. Methods Ectopic endometrial tissues and normal tissue specimens were collected, and the expression of NUCKS1, NF-κB and PI3K was detected by RT-qPCR and immunohistochemistry. Inhibition of NUCKS1 in hEM15A cells, study the changes in cell viability, apoptosis, migration and protein expression by CCK8 assay, flow cytometry, wound-healing assay, western blot and ELISA techniques. The comparison of differences between the two groups was implemented using the unpaired sample t test or the Wilcoxon signed rank sum test. One-way analysis of variance or Kruskal-wallis test was used for comparisons among the three groups. Results (1) NUCKS1 is highly expressed in endometriosis tissues. (2) Inhibition of NUCKS1 decreases cell viability and capability of migration, and increases apoptosis in endometriosis cells. (3) Expressions of NF-κB and PI3K are increased in endometriosis tissues, and inhibition of NUCKS1 decreases the expression levels of PI3K and NF-κB in endometriosis cells. (4) Inhibition of NUCKS1 decreases the expression of VEGF. Conclusion (1) NUCKS1 is overexpressed in endometriosis, and inhibition of NUCKS1 inhibits cell viability and capability of migration, and increases apoptosis. (2) NUCKS1 promotes the progress of endometriosis through activating PI3K and NF-κB pathways, and VEFG is also involved in this process.

show abstract

“…LMNA (lamin A/C) [187], PFKFB3 [188], TRAF2 [189], ADORA2B [190], ACADS (acyl-CoA dehydrogenase short chain) [191], IRF7 [192], ASL (argininosuccinatelyase) [193], IL15 [194], PIK3R1 [195], OSM (oncostatin M) [196], SOCS3 [197], CHD9 [198], IFI44L [199], GNLY (granulysin) [200], FLNB (filamin B) [201], IL1R1 [202], SETD3 [203], TP53 [204], BRD4 [205], COX2 [206], HSP90AB1 [207], SLC7A1 [208], ND2 [209] and COX1 [210] have been reported to be associated with hypertension. LMNA (lamin A/C) [211], INPP5K [212], SCYL1 [213], AKR7A2 [214], TRAF2 [215], SLC11A2 [216], NEU1 [217], SNAP29 [218], DUSP22 [219], P2RX4 [220], ADORA2B [221], NAXD (NAD(P)HX dehydratase) [222], FEZ1 [223], TBK1 [224], GRN (granulin precursor) [225], ATG4D [226], CTSD (cathepsin D) [227], PPP2R5D [228], IRF7 [229], ACAA1 [230], S1PR1 [231], PGRMC1 [169], MAPK14 [232], IL15 [233], OSM (oncostatin M) [234], GBA (glucosylceramidase beta) [235], SOCS3 [236], GLUL (glutamate-ammonia ligase) [175], MON1A [237], LMAN2L [238], RHOC (ras homolog family member C) [239], TUBB2A [240], CHP1 [241], TP53 [242], ATXN1 [243], JARID2 [244], IFNG (interferon gamma) [245], NUCKS1 [246], CTBP1 [247], DNAJC3 [180], ATP6 [248], BRD4 [249], EIF4B [250], COX2 [251], SFPQ (splicing factor proline and glutamine rich) [252], PCBP1 [253], RPL5 [254], HSP90AB1 [255], TRA2A […”

Section: Discussionmentioning

confidence: 99%

Bioinformatics Analysis of next generation sequencing data for Risk Prediction in Patients with Type 1 diabetes mellitus

Vastrad¹,

Vastrad²

2022

Preprint

View full text Add to dashboard Cite

Type 1 diabetes mellitus (T1DM) comprise the most common forms of autoimmune disease. The aim of this investigation was to apply a bioinformatics approach to reveal related pathways or genes involved in the development of T1DM. The next generation sequencing (NGS) dataset GSE182870 was downloaded from the gene expression omnibus (GEO) database. Differentially expressed gene (DEG) analysis was performed using DESeq2. The g:Profiler was utilized to analyze the functional enrichment, gene ontology (GO) and REACTOME pathway of the differentially expressed genes. Protein-protein interaction (PPI) network, modules, miRNA-hub gene regulatory network, and TF-hub gene regulatory network were conducted via comprehensive target prediction and network analyses. Finally, hub genes were validated by using receiver operating characteristic curve analysis. A total of 860 DEGs were screened out from NGS dataset, among which 477 genes were up regulated and 383 genes were down regulated. GO enrichment analysis indicated that up regulated genes were mainly involved in cellular metabolic process, intracellular anatomical structure and catalytic activity, and the down regulated genes were significantly enriched in cellular nitrogen compound biosynthetic process, protein containing complex and heterocyclic compound binding. REACTOME pathway enrichment analysis showed that the up regulated genes were mainly enriched in metabolism of carbohydrates and the down regulated genes were significantly enriched in metabolism of RNA. A PPI network, modules, miRNA-hub gene regulatory network and TF-hub gene regulatory network were constructed, and hub genes (MAPK14, RHOC, MAD2L1, TAF1, TRAF2, HSP90AA1, TP53, HSP90AB1, UBA52 and RACK1) were identified. This study provides further insights into the underlying pathogenesis of T1DM.

show abstract

Functional association between NUCKS1 gene and Parkinson disease: A potential susceptibility biomarker

Cited by 12 publications

References 37 publications

Parkinson’s disease risk enhancers in microglia

Parkinson’s disease risk enhancers in microglia

NUCKS1 is Overexpressed in Endometriosis

Bioinformatics Analysis of next generation sequencing data for Risk Prediction in Patients with Type 1 diabetes mellitus

Contact Info

Product

Resources

About