Functional assessment after sciatic nerve injury in a rat model

Dinh, Paul; Hazel, Antony; Palispis, Winnie A.; Suryadevara, Sourabh; Gupta, Ranjan

doi:10.1002/micr.20685

Cited by 84 publications

(49 citation statements)

References 36 publications

(41 reference statements)

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“…Finally, it has also been suggested that the BBB scale, a method commonly used for the study of spinal cord injury and regeneration (Basso et al, 1995), can be also a valuable additional method for the assessment of sciatic nerve injury and regeneration too (Dinh et al, 2009). This method is based on a 21 point scale based on the analysis of specific components of functional behavior, such as the limb movement, paw placement/position and stepping.…”

Section: Functional Assessmentmentioning

confidence: 99%

The sciatic nerve injury model in pre-clinical research

Geuna

2015

Journal of Neuroscience Methods

111

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In the pre-clinical view, the study of peripheral nerve repair and regeneration still needs to be carried out in animal models due to the structural complexity of this organ which can be only partly simulated in vitro. The far most used experimental model is based on the injury of the sciatic nerve, the largest nerve trunk in mammals. In this paper, the potential application of the sciatic nerve injury model in pre-clinical research is critically reviewed. This paper is aimed at helping researchers in properly employing this in vivo model for the study of nerve repair and regeneration as well as interpreting the results in a clinical translation perspective.

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Section: Functional Assessmentmentioning

confidence: 99%

The sciatic nerve injury model in pre-clinical research

Geuna

2015

Journal of Neuroscience Methods

111

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“…As described in a previous study (Liu et al, 2007;Dinh et al, 2009), the rats were allowed conditioning trials on an 8.2 x 50 cm walking track with a piece of white paper at the bottom of the track. The hind feet were dipped in red ink, leaving prints on the white paper.…”

Section: Determination Of Sciatic Functional Index (Sfi)mentioning

confidence: 99%

“…Therefore, in this study, we used the gastrocnemius muscle atrophy model caused by rat sciatic nerve crush. Following the progression of muscle atrophy and recovery, we determined the sciatic nerve functional index (SFI) (Dinh et al, 2009) and took blood samples for each rat at different postoperative time points. The blood was collected to detect the expression of myostatin and follistatin using ELISA methods.…”

Section: Introductionmentioning

confidence: 99%

A potential indicator of denervated muscle atrophy: the ratio of myostatin to follistatin in peripheral blood

Wang²,

Yang³

et al. 2011

Genet. Mol. Res.

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ABSTRACT. Myostatin is a secreted negative regulator of muscle mass, and follistatin antagonizes the function of several members of the TGF-b family, including myostatin. Previously, myostatin expression was found to be closely associated with atrophy of the gastrocnemius muscle, showing a linear correlation, after sciatic nerve injury. In this study, we investigated the possibility of myostatin being an indicator of denervated muscle atrophy. ELISA was used to detect the concentration of myostatin and follistatin in sera collected from individual rats at different times after sciatic nerve crush. A strong correlation was shown between the expression level of secreted myostatin in circulation and the wet weight ratio of the gastrocnemius muscle. The ratio of follistatin/ myostatin could be used to monitor the progress of target muscle ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research (2011) Ahead of Print A potential indicator of denervated muscle atrophy 3915 atrophy and recovery. Our study provides a potential serological test to detect denervated muscle atrophy for clinical purposes.

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“…Although BBB is widely used to assess functional recovery in spinal cord injured animals, however, it has been demonstrated that it could be most useful in assessment of never repair processes in peripheral nerve injuries [25]. Scores of 0 and 21 were given when there were no spontaneous movement and normal movement, respectively.…”

Section: Behavioral Testingmentioning

confidence: 99%

Peripheral Nerve Regeneration using Silicone Rubber Chamber Combined by Local Administration of Cyclosporin a in Streptozotocin Induced Diabetic Rats

Mohammadi¹

2015

Int J Oral Craniofac Sci

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Introduction: The repair of peripheral nerve injuries is still one of the most challenging tasks and concerns in neurosurgery. Effect of cyclosporin (CsA) loaded silicon conduit as an in situ delivery system of CsA in bridging the defects was studied using a sciatic nerve regeneration model in diabetic rats. Methods:A 10-mm sciatic nerve defect was bridged using a silicon conduit (SIL/CsA) filled with 10 µL carrier-drug dilution (10 ng/mL CsA). In control group (SIL), the conduit was filled with the same volume of the phosphate buffered solution. The regenerated fibers were studied 4, 8, 12 and 16 weeks after surgery. Result:The functional and electrophysiological studies confirmed faster recovery of the regenerated axons in CsA treated than control group (P < 0.05). The mean ratios of gastrocnemius muscles weight were measured. There was statistically significant difference between the muscle weight ratios of SIL/CsA and SIL groups (P<0.05). Morphometric indices of regenerated fibers showed number and diameter of the myelinated fibers in SIL/CsA were significantly higher than in control group. Conclusion:Cyclosporin when loaded in a silicon conduit resulted in improvement of functional recovery and quantitative morphometric indices of sciatic nerve in diabetic rats.

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Functional assessment after sciatic nerve injury in a rat model

Cited by 84 publications

References 36 publications

The sciatic nerve injury model in pre-clinical research

The sciatic nerve injury model in pre-clinical research

A potential indicator of denervated muscle atrophy: the ratio of myostatin to follistatin in peripheral blood

Peripheral Nerve Regeneration using Silicone Rubber Chamber Combined by Local Administration of Cyclosporin a in Streptozotocin Induced Diabetic Rats

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