Functional Aspects of Fish Mucosal Lectins—Interaction with Non-Self

Brinchmann, Monica F.; Patel, Deepti Manjari; Pinto, Nevil; Iversen, Martin Haugmo

doi:10.3390/molecules23051119

Cited by 40 publications

(24 citation statements)

References 68 publications

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“…Zebrafish produce antimicrobial peptides, of which β-defensins, cathelicidins, hepcidins, and histone-derived peptides are also found in mammals, and piscidins are fish specific (Katzenback, 2015;Zou, Mercier, Koussounadis, & Secombes, 2007). Secreted peptidoglycan recognition proteins (Chang, Nie, & Wei, 2007) and antibacterial lectins functionally similar to mammalian pore forming Ctype lectins (Brinchmann, Patel, Pinto, & Iversen, 2018) also contribute to zebrafish intestinal mucosal immunity. Secreted antimicrobial factors are expressed throughout the zebrafish intestine, both in larvae and adults (Oehlers et al, 2011a).…”

Section: Innate Immunity and The Gi Tractmentioning

confidence: 99%

The zebrafish as a model for gastrointestinal tract–microbe interactions

Flores

Nguyen

Odem

et al. 2020

Cellular Microbiology

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The zebrafish (Danio rerio) has become a widely used vertebrate model for bacterial, fungal, viral, and protozoan infections. Due to its genetic tractability, large clutch sizes, ease of manipulation, and optical transparency during early life stages, it is a particularly useful model to address questions about the cellular microbiology of host-microbe interactions. Although its use as a model for systemic infections, as well as infections localised to the hindbrain and swimbladder having been thoroughly reviewed, studies focusing on host-microbe interactions in the zebrafish gastrointestinal tract have been neglected. Here, we summarise recent findings regarding the developmental and immune biology of the gastrointestinal tract, drawing parallels to mammalian systems. We discuss the use of adult and larval zebrafish as models for gastrointestinal infections, and more generally, for studies of host-microbe interactions in the gut. K E Y W O R D S Danio rerio, gastrointestinal tract, host-pathogen interactions, infection model, microbiome, microbiota, zebrafish

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Section: Innate Immunity and The Gi Tractmentioning

confidence: 99%

The zebrafish as a model for gastrointestinal tract–microbe interactions

Flores

Nguyen

Odem

et al. 2020

Cellular Microbiology

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“…As primary vertebrate species, teleosts possess both innate immune and adaptive immune systems (54). The innate immune system of teleosts has more diverse immune molecules than that of mammals, including lectins, complement, and NK cell receptors (30,55). However, the adaptive immune system of teleosts has fewer Ig types than that of mammals (56,57), a lack of Ab classswitch recombination, and low Ab affinity maturation (30).…”

Section: Discussionmentioning

confidence: 99%

CXCR4s in Teleosts: Two Paralogous Chemokine Receptors and Their Roles in Hematopoietic Stem/Progenitor Cell Homeostasis

Zhu

Shen

et al. 2020

The Journal of Immunology

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Hematopoietic stem/progenitor cells (HSPCs) generate the entire repertoire of immune cells in vertebrates and play a crucial role during infection. Although two copies of CXC motif chemokine receptor 4 (CXCR4) genes are generally identified in teleosts, the function of teleost CXCR4 genes in HSPCs is less known. In this study, we identified two CXCR4 genes from a teleost, ayu (Plecoglossus altivelis), named PaCXCR4a and PaCXCR4b. PaCXCR4b was constitutively expressed in ayu HSPCs, whereas PaCXCR4a was induced by LPS treatment. The stromal-derived factor-1-binding activity of CXCR4b was significantly higher than that of CXCR4a, whereas the LPS-binding activity of CXCR4a was significantly higher than that of CXCR4b in the teleosts ayu, large yellow croaker (Larimichthys crocea), and tiger puffer (Takifugu rubripes). CXCR4a + HSPCs were mobilized into blood by LPS, whereas CXCR4b + HSPCs were mobilized by leukocyte cell-derived chemotaxin-2. PaSDF-1 and PaCXCR4b, but not PaCXCR4a, inhibited HSPC proliferation by regulating reactive oxygen species levels. Compared with PaCXCR4b + HSPCs, PaCXCR4a + HSPCs preferentially differentiated into myeloid cells in ayu by maintaining high stem cell leukemia expression. These data suggest that the two copies of CXCR4s achieve a division of labor in the regulation of teleost HSPC homeostasis, supporting the concept that subfunctionalization after gene duplication in teleosts may stabilize the immune system.

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“…Spot D15 was identified as natterin, a protein with lectin like domain and a toxic domain with kinogenase activity 30,31 . In skin, the lectin like domain could recognise pathogens and the toxin domain could cause lysis of pathogenic microbes.…”

Section: Discussionmentioning

confidence: 99%

Proteomic and structural differences in lumpfish skin among the dorsal, caudal and ventral regions

Patel

Bhide

et al. 2019

Sci Rep

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Fish skin is a vital organ that serves a multitude of functions including mechanical protection, homeostasis, osmoregulation and protection against diseases. The expression of skin proteins changes under different physiological conditions. However, little is known about differences in protein expression among various body sites in naïve fish. The objectives of this work is to study potential differences in protein and gene expression among dorsal, caudal and ventral regions of lumpfish skin employing 2D gel based proteomics and real-time PCR and to assess structural differences between these regions by using Alcian blue and Periodic acid Schiff stained skin sections. The proteins collagen alfa-1, collagen alfa-2, heat shock cognate 71 kDa, histone H4, parvalbumin, natterin-2, 40S ribosomal protein S12, topoisomerase A and topoisomerase B were differentially expressed among the three regions. mRNA expression of apoa1 , hspa8 and hist1h2b showed significant differences between regions. Skin photomicrographs showed differences in epidermal thickness and goblet cell counts. The ventral region showed relatively high protein expression, goblet cell count and epidermal thickness compared to dorsal and caudal regions. Overall, this study provides an important benchmark for comparative analysis of skin proteins and structure between different parts of the lumpfish body.

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Functional Aspects of Fish Mucosal Lectins—Interaction with Non-Self

Cited by 40 publications

References 68 publications

The zebrafish as a model for gastrointestinal tract–microbe interactions

The zebrafish as a model for gastrointestinal tract–microbe interactions

CXCR4s in Teleosts: Two Paralogous Chemokine Receptors and Their Roles in Hematopoietic Stem/Progenitor Cell Homeostasis

Proteomic and structural differences in lumpfish skin among the dorsal, caudal and ventral regions

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