Functional Aspects of Creatine Kinase in Brain

Hemmer, Wolfram; Wallimann, Theo

doi:10.1159/000111342

Cited by 159 publications

(101 citation statements)

References 66 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The presence of brain type B CK in Bergmann glial cells and astrocytes is very likely related to the energy requirements for ion homeostasis in the brain, as well as for metabolite and neurotransmitter trafficking between glial cells and neurons. CK and its substrates, creatine and phosphocreatine, constitute an intricate cellular energy buffering and transport system connecting sites of energy production (mitochondria) with sites of energy consumption (Hemmer and Wallimann, 1993). Creatine stabilizes mitochondrial CK and inhibits opening of the mitochondrial transition pore (O'Gorman et al, 1996).…”

Section: Discussionmentioning

confidence: 99%

Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes

et al. 2008

View full text Add to dashboard Cite

Delta-9-tetrahydrocannabinol (Δ 9 -THC), the main psychoactive component of marijuana, is known to dysregulate various immune responses. Cannabinoid (CB)-1 and -2 receptors are expressed mainly on cells of the central nervous system (CNS) and the immune system. The CNS is the primary target of cannabinoids and astrocytes are known to play a role in various immune responses. Thus we undertook this investigation to determine the global molecular effects of cannabinoids on normal human astrocytes (NHA) using genomic and proteomic analyses. NHA were treated with Δ 9 -THC and assayed using gene microarrays and two-dimensional (2D) difference gel electrophoresis (DIGE) coupled with mass spectrometry (MS) to elucidate their genomic and proteomic profiles respectively. Our results show that the expression of more than 20 translated protein gene products from NHA was differentially dysregulated by treatment with Δ 9 -THC compared to untreated, control NHA.

show abstract

Section: Discussionmentioning

confidence: 99%

Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes

et al. 2008

View full text Add to dashboard Cite

show abstract

“…creatine phosphate) and CK isoenzymes play a central role in energy transduction in tissues with large fluctuating energy demands like the brain. 28 Using magnetisation transfer techniques, creatine kinase activity was shown to be reduced in rat brains following chronic alcohol intoxication. 29 This together with the decreased expression of CK-B in alcoholic BA9 white matter suggests a role for CK-B in alcohol-related brain damage.…”

Section: Discussionmentioning

confidence: 99%

Differential protein expression in the prefrontal white matter of human alcoholics: a proteomics study

et al. 2005

View full text Add to dashboard Cite

Neuroimaging and post-mortem studies indicate that chronic alcohol use induces global changes in brain morphology, such as cortical and subcortical atrophy. Recent studies have shown that frontal lobe structures are specifically susceptible to alcohol-related brain damage and shrinkage in this area is largely due to a loss of white matter. This may explain the high incidence of cognitive dysfunction observed in alcoholics. Using a proteomics-based approach, changes in protein expression in the dorsolateral prefrontal region (BA9) white matter were identified in human alcoholic brains. Protein extracts from the BA9 white matter of 25 human brains (10 controls; eight uncomplicated alcoholics; six alcoholics complicated with hepatic cirrhosis; one reformed alcoholic) were separated using two-dimensional gel electrophoresis. Overall, changes in the relative expression of 60 proteins were identified (Po0.05, ANOVA) in the alcoholic BA9 white matter. In total, 18 protein spots have been identified using MALDI-TOF; including hNP22, a-internexin, transketolase, creatine kinase chain B, ubiquitin carboxy-terminal hydrolase L1 and glyceraldehyde-3-phosphate dehydrogenase. Several of these proteins have been previously implicated in alcohol-related disorders and brain damage. By identifying changes in protein expression in this region from alcoholics, hypotheses may draw upon more mechanistic explanations as to how chronic ethanol consumption causes white matter damage.

show abstract

“…Located in the cytoplasm of glial cells 38 and GABAergic interneurons, 39 CKB reversibly catalyses the transfer of phosphate between ATP and various phosphogens such as creatine phosphate, 40 important in the brain for habituation, spatial learning and behaviour. 41 Cells with high CKB expression are known to be resistant to neurological insults, such as glucose and oxygen deprivation 39 and as such the increased CKB expression in the ACC gray matter may protect cells from oxidative damage observed in the disease state.…”

Section: Metabolismmentioning

confidence: 99%

A proteome analysis of the anterior cingulate cortex gray matter in schizophrenia

et al. 2006

View full text Add to dashboard Cite

The Anterior Cingulate Cortex (ACC, Brodmans Area 24) is implicated in the pathogenesis of schizophrenia due to its normal functions and connectivity together with reports of structural, morphological and neurotransmitter aberrations within this brain area in the disease state. Two-dimensional gel electrophoresis (2DE) was employed to scan and compare the ACC gray matter proteomes between schizophrenia (n = 10) and control (n = 10) post-mortem human tissue. This proteomic approach has detected 42 protein spots with altered levels in the schizophrenia cohort, which to our knowledge is the first proteomic analysis of the ACC in schizophrenia. Thirty nine of these proteins were subsequently identified using mass spectrometry and functionally classified into metabolism and oxidative stress, cytoskeletal, synaptic, signalling, trafficking and glial-specific groups. Some of the identified proteins have previously been implicated in the disease pathogenesis and some offer new insights into schizophrenia. Investigating these proteins, the genes encoding these proteins, their functions and interactions may shed light on the molecular mechanisms underlying the heterogeneous symptoms characteristic of schizophrenia.

show abstract

Functional Aspects of Creatine Kinase in Brain

Cited by 159 publications

References 66 publications

Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes

Genomic and proteomic analysis of the effects of cannabinoids on normal human astrocytes

Differential protein expression in the prefrontal white matter of human alcoholics: a proteomics study

A proteome analysis of the anterior cingulate cortex gray matter in schizophrenia

Contact Info

Product

Resources

About