Functional Antagonism between High Temperature Requirement Protein A (HtrA) Family Members Regulates Trophoblast Invasion

Chen, Yao Yu; Chuang, Pei Yun; Chen, Chie Pein; Chiu, Yueh Ho; Cheong, Mei Leng; Huang, Jyun Yuang; Kuo, Pao Lin; Chen, Hung−Wen

doi:10.1074/jbc.m114.576744

Cited by 32 publications

(25 citation statements)

References 27 publications

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“…Conversely, a decrease in the anti-HtrA4 response in the CM sera could be caused by the interaction of the secreted HtrA1 and HtrA3 proteases with the HtrA4 protein in the ECM. Additionally, it has been shown that HtrA1 and HtrA3 interact with HtrA4 and degrade it (Singh et al, 2011;Chen et al, 2014). The changes in the HtrA proteins' concentrations are probably restricted to the areas where the MCs accumulate, since there were no significant changes in the serum levels of the HtrA1/3/4 proteins between the CM and control groups (Fig.…”

Section: Discussionmentioning

confidence: 90%

Immune response against HtrA proteases in children with cutaneous mastocytosis

Renke

Kędzierska‐Mieszkowska

Lange

et al. 2018

Acta Biochim Pol

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Mast cells play an important role in both, the innate and adaptive immunity, however, clonal proliferation of abnormal mast cells in various organs leads to mastocytosis. A skin variant of the disease, cutaneous mastocytosis (CM) is the most frequent form of mastocytosis in children. HtrA proteases are modulators of important cellular processes, including cell signaling and apoptosis, and are related to development of several pathologies. The above and the observation that mast cells constitutively release the HtrA1 protein, prompted us to investigate a possible involvement of the HtrA proteins in pediatric CM. Levels of the serum autoantibodies (IgG) against the recombinant HtrA proteins (HtrA1-4) in children with CM (n=36) and in healthy controls (n=62) were assayed. Anti-HtrA IgGs were detected using enzyme linked immunosorbent assay (ELISA) and Western-blotting. In the CM sera, levels of the anti-HtrA1 and anti-HtrA3 autoantibodies were significantly increased when compared to the control group, while the HtrA protein levels were comparable. No significant differences in the anti-HtrA2 IgG level were found; for the anti-HtrA4 IgGs lower levels in CM group were revealed. In healthy children, the IgG levels against the HtrA1, -3 and -4 increased significantly with the age of children; no significant changes were observed for the anti-HtrA2 IgG. Our results suggest involvement of the HtrA1 and HtrA3 proteins in pediatric CM; involvement of the HtrA4 protein is possible but needs to be investigated further. In healthy children, the autoantibody levels against HtrA1, -3 and -4, but not against HtrA2, increase with age.

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Section: Discussionmentioning

confidence: 90%

Immune response against HtrA proteases in children with cutaneous mastocytosis

Renke

Kędzierska‐Mieszkowska

Lange

et al. 2018

Acta Biochim Pol

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“…Indeed, HtrA4, like HtrA1/3 has been found to be a protease associated with pregnancy; it is implicated in embryo implantation and morphogenesis of placenta, and promotes trophoblast invasion [89]. Recently, Chen et al [85] revealed a novel regulatory mechanism of trophoblast invasion through physical and functional interaction between the HtrA family members. As already mentioned, they found that the decidua-secreted HtrA1 and HtrA3 antagonize the HtrA4-mediated trophoblast invasion and that HtrA4 is subjected to proteolytic cleavage by these proteases.…”

Section: Human Htra Proteins In Cellular Physiology and Pathogenesismentioning

confidence: 98%

“…It has been shown, using cultured cell lines that decidua-secreted HtrA3 is an important element of this regulatory system. HtrA3, together with HtrA1, antagonizes HtrA4-mediated trophoblast invasion by degrading HtrA4 [84,85].…”

Section: Human Htra Proteins In Cellular Physiology and Pathogenesismentioning

confidence: 99%

Structural insights into the activation mechanisms of human HtrA serine proteases

Żurawa-Janicka

Wenta

Jarzab

et al. 2017

Archives of Biochemistry and Biophysics

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“…High temperature requirement A4 (HTRA4) is a secreted serine protease highly expressed in the invasive extravillous trophoblasts that invade decidua. HtrA1 and HtrA3 interact with and degrade HtrA4 and thereby inhibit trophoblast-like JAR cell invasion (Chen et al 2014). In our study, Prss56 is highly expressed in the primary decidual zone on day 5 of pregnancy, which is co-localized with COX-2 and mPGES-1, suggesting that Prss56 may regulate decidualization via stimulating COX-2 and mPGES-1 as trypsin does.…”

Section: Discussionmentioning

confidence: 49%

HB-EGF regulates Prss56 expression during mouse decidualization via EGFR/ERK/EGR2 signaling pathway

Liu

Gao

Liu

et al. 2017

Journal of Endocrinology

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Embryo implantation and decidualization are key steps for successful reproduction. Although numerous factors have been identified to be involved in embryo implantation and decidualization, the mechanisms underlying these processes are still unclear. Based on our preliminary data, Prss56, a trypsin-like serine protease, is strongly expressed at implantation site in mouse uterus. However, the expression, regulation and function of Prss56 during early pregnancy are still unknown. In mouse uterus, is strongly expressed in the subluminal stromal cells at implantation site on day 5 of pregnancy compared to inter-implantation site. Under delayed implantation, expression is undetected. After delayed implantation is activated by estrogen, Prss56 is obviously induced at implantation site. Under artificial decidualization, Prss56 signal is seen at the primary decidual zone at the initial stage of artificial decidualization. When stromal cells are induced for decidualization, expression is significantly elevated. expression under decidualization is suppressed by siRNA. In cultured stromal cells, HB-EGF markedly stimulates expression through EGFR/ERK pathway. Based on promoter analysis, we also showed that Egr2 is involved in Prss56 regulation by HB-EGF. Collectively, expression at implantation site is modulated by HB-EGF/EGFR/ERK signaling pathway and involved in mouse decidualization.

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Functional Antagonism between High Temperature Requirement Protein A (HtrA) Family Members Regulates Trophoblast Invasion

Cited by 32 publications

References 27 publications

Immune response against HtrA proteases in children with cutaneous mastocytosis

Immune response against HtrA proteases in children with cutaneous mastocytosis

Structural insights into the activation mechanisms of human HtrA serine proteases

HB-EGF regulates Prss56 expression during mouse decidualization via EGFR/ERK/EGR2 signaling pathway

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