Functional and structural organization of chlorophyll in the developing photosynthetic membranes of Euglena gracilis Z. V-separation and characterization of pigment-protein complexes of the differentiated thylakoids

Pineau, Bernard; Dubertret, G.; Schantz, Rodolphe

doi:10.1007/bf00032790

Cited by 11 publications

(2 citation statements)

References 29 publications

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“…Chlorophyll is bound in the thylakoid membrane to specific chlorophyll binding proteins which are associated with the photosystem I reaction center complex, CP I, the photosystem II reaction center complex, CPa, and the light harvesting complex proteins associated with photosystem I and II (Cunningham and Schiff 1986a, b;Schantz 1987, 1988;Ortiz and Stutz 1980;Pineau et al 1985;Yi et al 1985). Proteins of the electron transport chain are also in the thylakoid membrane.…”

Section: Photocontrol Of Chloroplast Developmentmentioning

confidence: 99%

Photo and Nutritional Regulation of Euglena Organelle Development

Schwartzbach

2017

Advances in Experimental Medicine and Biology

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Euglena can use light and CO, photosynthesis, as well as a large variety of organic molecules as the sole source of carbon and energy for growth. Light induces the enzymes, in this case an entire organelle, the chloroplast, that is required to use CO as the sole source of carbon and energy for growth. Ethanol, but not malate, inhibits the photoinduction of chloroplast enzymes and induces the synthesis of the glyoxylate cycle enzymes that comprise the unique metabolic pathway leading to two carbon, ethanol and acetate, assimilation. In resting, carbon starved cells, light mobilizes the degradation of the storage carbohydrate paramylum and transiently induces the mitochondrial proteins required for the aerobic metabolism of paramylum to provide the carbon and energy required for chloroplast development. Other mitochondrial proteins are degraded upon light exposure providing the amino acids required for the synthesis of light induced proteins. Changes in protein levels are due to increased and decreased rates of synthesis rather than changes in degradation rates. Changes in protein synthesis rates occur in the absence of a concomitant increase in the levels of mRNAs encoding these proteins indicative of photo and metabolic control at the translational rather than the transcriptional level. The fraction of mRNA encoding a light induced protein such as the light harvesting chlorophyll a/b binding protein of photosystem II, (LHCPII) associated with polysomes in the dark is similar to the fraction associated with polysomes in the light indicative of photoregulation at the level of translational elongation. Ethanol, a carbon source whose assimilation requires carbon source specific enzymes, the glyoxylate cycle enzymes, represses the synthesis of chloroplast enzymes uniquely required to use light and CO as the sole source of carbon and energy for growth. The catabolite sensitivity of chloroplast development provides a mechanism to prioritize carbon source utilization. Euglena uses all of its resources to develop the metabolic capacity to utilize carbon sources such as ethanol which are rarely in the environment and delays until the rare carbon source is no longer available forming the chloroplast which is required to utilize the ubiquitous carbon source, light and CO.

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Section: Photocontrol Of Chloroplast Developmentmentioning

confidence: 99%

Photo and Nutritional Regulation of Euglena Organelle Development

Schwartzbach

2017

Advances in Experimental Medicine and Biology

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“…Chlorophyll is found in the thylakoid membrane bound to specific chlorophyll binding proteins which are associated with the photosystem I reaction center complex, C P I, the photosystem I1 reaction center complex, CPa, and the light harvesting chlorophyll aib binding proteins of photosystem 11, LHCPII (Ortiz and Stutz, 1980;Pineau et al, 1985;Yi et al, 1985;Cunningham and Schiff, 1986a, b). The thylakoid membrane also contains the proteins PAP 51:Z-6' of the electron transport chain.…”

Section: Photocontrol Of the Synthesis Of Chloroplast Proteinsmentioning

confidence: 99%

PHOTOCONTROL OF ORGANELLE BIOGENESIS IN Euglena

Schwartzbach

1990

Photochem & Photobiology

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Characterization of cDNA sequences for LHCI apoproteins in Euglena gracilis: The mRNA encodes a large precursor containing several consecutive divergent polypeptides

Houlné

Schantz

1988

Mol Gen Genet

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Screening of a lambda gt11 cDNA expression library with antibodies directed against LHCII allowed the detection of several clones which differ markedly from the previously described cDNAs encoding LHCII apoproteins. A Northern analysis revealed a transcript size of 4.2 kb, whereas clones encoding LHCII hybridize with mRNAs in the range of 7.5 kb. Nucleotide sequencing of 2 clones showed open reading frames of 530 and 331 codons, respectively. Within these reading frames, 5 analogous motifs can be delimited corresponding to coding regions for around 180 amino acids (molecular weight, 18-19 kDa). The 5 segments share between 50% and 80% homology. Comparison with a tomato LHCI sequence indicates conserved regions at the two ends, and a central part highly divergent and containing a large deletion. By hybrid-selected translation, followed by immunoprecipitation with a monoclonal antibody directed against LHCI apoprotein, a protein of around 100 kDa is obtained. In vivo, the same antibody recognizes peptides of around 20 kDa. These results, coupled with our previous observations concerning LHCII, confirm that in Euglena at least, some chloroplast proteins encoded in the nucleus are synthesized by large mRNAs containing the information for several consecutive divergent peptides. Implications for processing and chloroplast import are discussed.

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Functional and structural organization of chlorophyll in the developing photosynthetic membranes of Euglena gracilis Z. V-separation and characterization of pigment-protein complexes of the differentiated thylakoids

Cited by 11 publications

References 29 publications

Photo and Nutritional Regulation of Euglena Organelle Development

Photo and Nutritional Regulation of Euglena Organelle Development

PHOTOCONTROL OF ORGANELLE BIOGENESIS IN Euglena

Characterization of cDNA sequences for LHCI apoproteins in Euglena gracilis: The mRNA encodes a large precursor containing several consecutive divergent polypeptides

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