Functional and structural characterization of two <i>Bacillus megaterium</i> nitroreductases biotransforming the herbicide mesotrione

Carles, Louis; Besse-Hoggan, Pascale; Joly, Muriel; Vigouroux, Armelle; Morera, S.; Batisson, Isabelle

doi:10.1042/bj20151366

Cited by 21 publications

(19 citation statements)

References 46 publications

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“…Moreover, a proteomic study of Bacillus megaterium strain Mes11 revealed a network of mesotrione‐adaptative proteins mainly involved in stress response, central metabolism and storage mechanisms . More recently, the identification of mesotrione‐degrading enzymes (NfrA1 and NfrA2/YcnD) in this strain, led the way to new biomarkers in order to foresee the ability of environment to transform this molecule .…”

Section: Fate Of Mesotrione In the Environmentmentioning

confidence: 99%

Mesotrione Herbicide: Efficiency, Effects, and Fate in the Environment after 15 Years of Agricultural Use

Carles

Joly

2017

CLEAN Soil Air Water

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Mesotrione (2‐[4‐(methylsulfonyl)‐2‐nitrobenzoyl]‐cylohexane‐1,3‐dione) is an herbicide brought to the market 15 years ago and commonly used on maize cultures. This review summarizes the results obtained from worldwide scientific studies on its efficiency, effects, and fate in environment, in order to bring first overall conclusions about its safety of use. The comprehensive review of the available scientific literature reveals that mesotrione applied as recommended ensures the desired effects on sensitive crops, mainly in post‐emergence application, while exhibiting favorable toxicological, and environmental profile. According to the studies realized so far, mesotrione presents no significant risks to humans and other non‐target organisms or to the environment. Its degradation by soil microorganisms is fast, ensuring a low possibility to retrieve mesotrione in ground water. Its terminal residues in soil, maize, grain, and maize straw are below the limit of quantification. All these results suggest that mesotrione applied as directed ensures the respect of current environmental standards and food safety. Future prospects should focus on its associated pesticides when applied in mixtures on field, and on the detection, quantification, and risk assessment of its transformation products in order to exclude any toxicological synergistic effects.

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Section: Fate Of Mesotrione In the Environmentmentioning

confidence: 99%

Mesotrione Herbicide: Efficiency, Effects, and Fate in the Environment after 15 Years of Agricultural Use

Carles

Joly

2017

CLEAN Soil Air Water

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“…The PCA analysis did not reveal significant volatolome difference in B. megaterium Mes11 whether they were exposed to the sulcotrione or not. B. megaterium Mes11 was characterized as an efficient mesotrione-degrading strain (Batisson et al, 2009; Bardot et al, 2015; Carles et al, 2016), a selective herbicide belonging to the triketone family like the sulcotrione. Briefly, B. megaterium Mes11 isolated from soils was able to completely and very rapidly (about 1 h at 30 mg/L) biotransform the mesotrione into 2-amino-4methylsulfonylbenzoic acid (AMBA) and 4-methylsufonyl-2-nitrobenzoic acid (MNBA) in its culture growth (Batisson et al, 2009; Carles et al, 2016) by nitro-reduction and hydrolysis.…”

Section: Discussionmentioning

confidence: 99%

“…Therefore, in the present work, we designed an experimental volatolomic test devoted to identify indirect VOC markers of exposure of two soil bacterial strains to three different pesticides (deltamethrine, cypermethrine and sulcotrione). We choose to work with Pseudomonas fluorescens strain SG-1 and Bacillus megaterium strain Mes11, two common soil strains characterized based on works on pesticide degradations and selected from enrichment culture conditions (Batisson et al, 2009; Bardot et al, 2015; Carles et al, 2016). Based on previous works dealing with the exposure of bacteria communities and other organisms to chemical contaminants (Berge et al, 2011; Bouhlel et al, 2017; Defois et al, 2017), we assumed that exposure to pesticides should influence the metabolic pathways of our two bacteria strains, resulting in specific changes in their volatolome.…”

Section: Introductionmentioning

confidence: 99%

Volatolomics in Bacterial Ecotoxicology, A Novel Method for Detecting Signatures of Pesticide Exposure?

Hidalgo¹,

Ratel²,

Mercier³

et al. 2019

Front. Microbiol.

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Volatile organic compounds (VOC) produced by microorganisms in response to chemical stressor showed recently increasing attention, because of possible environmental applications. In this work, we aimed to bring the first proof of concept that volatolomic (i.e., VOCs analysis) can be used to determine candidate VOC markers of two soil bacteria strains (Pseudomonas fluorescens SG-1 and Bacillus megaterium Mes11) exposure to pesticides. VOC determination was based on solid-phase microextraction (SPME) coupled with gas chromatography-mass spectrometry (GC-MS). Accordingly, we highlighted a set of bacterial VOCs modulated in each strains according to the nature of the pesticide used. Three out these VOCs were specifically modulated in P. fluorescens SG-1 when exposed with two pyrethroid pesticides (deltamethrine and cypermethrine): 2-hexanone; 1,3-ditertbutylbenzene and malonic acid, hexyl 3-methylbutyl ester. Our results thus suggest the possible existence of generic VOC markers of pyrethroids in this strain. Of particular interest, two out of these three VOCs, the 1,3-ditertbutylbenzene and the malonic acid, hexyl 3-methylbutyl ester were found also in B. megaterium Mes11 when exposed with cypermethrine. This result highlighted the possible existence of interspecific VOC markers of pyrethroid in these two bacteria. Altogether, our work underlined the relevance of volatolomic to detect signatures of pesticides exposure in microorganisms and more generally to microbial ecotoxicology. Based on these first results, considerations of volatolomics for the chemical risk assessment in environment such as soils can be indirectly explored in longer terms.

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“…Bacillus sp. strains (DURAND et al, 2006;BATISSON et al, 2009;CARLES et al, 2016) and Bradyrhizobium sp. SR1.…”

Section: Mesotrione Degradation As Affected By Soil Type and Applicatmentioning

confidence: 99%

“…and Escherichia coli (DURAND et al, 2006;PILEGGI et al, 2012;OLCHANHESKI et al, 2014;CARLES et al, 2016;ROMDHANE et al, 2016). Mesotrione degradation by Bacillus sp.…”

Section: Introductionmentioning

confidence: 99%

Environmental fate and behavior of mesotrione alone and mixed with S-metolachlor and terbuthylazine in Brazilian soils

Mendes¹

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Dados Internacionais de Catalogação na Publicação (CIP) Seção Técnica de Biblioteca -CENA/USP Mendes, Kassio FerreiraDestino ambiental e comportamento do mesotrione isolado e misturado com S-metolachlor e terbuthylazine em solos brasileiros / Environmental fate and behavior of mesotrione alone and mixed with S-metolachlor and terbuthylazine in Brazilian soils / Kassio Ferreira Mendes; orientador Valdemar Luiz Tornisielo. The mixture of herbicides is a widely used technique in weed control in several crops, including maize. However, interactions that might potentially result from mixing herbicides are a matter of constant concern and research. Soils are ideal settings to study transport and behavior of herbicides along gradients of physicochemical properties. The aim of this study was to evaluate sorption-desorption, leaching mineralization, biodegradation, and microbial respiration of mesotrione applied alone and mixed with S-metolachlor + terbuthylazine in samples from Brazilian sites cultivated with maize. The sorption-desorption of 14 C-labeled [cyclohexane-2-14 C] mesotrione was evaluated using the batch method, leaching was in glass columns, mineralization and degradation experiments were conducted using biometric flask, and microbial respiration was established according to the soil microorganisms: carbon transformation test with , respectively, with similar behavior across soils. Mesotrione sorption was influenced primarily by the clay mineral (CM) content and the soil pH. Leaching of mesotrione is relatively high in the tropical soils and correlates with the pH (R 2 = -0.84) and CM content (R 2 = 0.75) and may pose a potential groundwater contamination risk. From the 49 d laboratory incubation data, increased mineralization half-life of mesotrione were observed for the mixture of herbicides, ranging from a 4 d increase for the sandy loam soil to a 1 d increase in the sandy clay texture soils. Mesotrione degradation rate had a 2-fold increase in the sandy loam compared to the sandy clay soil. Two metabolites can be identified from mesotrione degradation, 4-methyl-sulfonyl-2-nitrobenzoic acid (MNBA) and 2-amino-4-methylsulfonyl benzoic acid (AMBA). For unamended soil -control (without herbicide), microbial activity followed similar behavior to amended soil with herbicides in total 14 CO 2 released and accumulated, ranging from 23 to 27%. The mode of application of mesotrione did not influence sorption, desorption or leaching. Mesotrione sorption was relatively low in soils, which creates the high potential for leaching in maize producing areas. Thus, recommendations for mesotrione application, without the prior knowledge of the soil physical and chemical properties can result in an inefficient weed control. Mesotrione biotransformation was relatively quick, indicating this herbicide has low persistence, and consequently, low residual effect on crops and weeds when present in similar soils to this present study. Microbial respiration for all treatments was slightly higher in the sandy clay compared with sa...

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Functional and structural characterization of two Bacillus megaterium nitroreductases biotransforming the herbicide mesotrione

Cited by 21 publications

References 46 publications

Mesotrione Herbicide: Efficiency, Effects, and Fate in the Environment after 15 Years of Agricultural Use

Mesotrione Herbicide: Efficiency, Effects, and Fate in the Environment after 15 Years of Agricultural Use

Volatolomics in Bacterial Ecotoxicology, A Novel Method for Detecting Signatures of Pesticide Exposure?

Environmental fate and behavior of mesotrione alone and mixed with S-metolachlor and terbuthylazine in Brazilian soils

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