Functional and structural characterization of IdnL7, an adenylation enzyme involved in incednine biosynthesis

Abstract

Edited by M. J. Romao, Universidade Nova de Lisboa, Portugal

Polyene Macrolactams from Marine and Terrestrial Sources: Structure, Production Strategies, Biosynthesis and Bioactivities

“…The second mode represented for incednine (35) biosynthesis [108][109][110][111], β-glutamate derived from α-L-glutamate by the action of glutamate 2,3-aminomutase is decarboxylated to (S)-β-aminobutyrate by the PLP-dependent β-glutamate decarboxylase IdnL3 (Figure 6). Then, the mentioned β-amino acid combined with acyltransferase (AT module) constructed a β-keto acyl intermediate.…”

“…Specific matching and protein combinations would need to be made to ensure the smooth coordination function of the key nodes' proteins in the PKS assembly line. To obtain the crystal structure [111,123] of biosynthetic enzymes X-ray diffraction, structural biology and physics methods were required. The surface interaction among proteins has already been observed using a Frozen Electron Microscope (FEM).…”

Structural Basis of Amide-Forming Adenylation Enzyme VinM in Vicenistatin Biosynthesis

“…VinM is conserved among biosynthetic gene clusters of other β-amino acid-containing macrolactam polyketides such as incednine and hitachimycin. , The VinM-type adenylation enzyme IdnL7 catalyzes the formation of an amide bond between 3-aminobutyryl-CP and l -alanine in incednine biosynthesis . The VinM-type adenylation enzyme HitE similarly catalyzes the formation of an amide bond between β-phenylalanyl-CP and l -alanine in hitachimycin biosynthesis .…”

“…The VinM-type adenylation enzyme HitE similarly catalyzes the formation of an amide bond between β-phenylalanyl-CP and l -alanine in hitachimycin biosynthesis . Thus, amide-forming adenylation enzymes are consistently used in the biosynthesis of β-amino acid-containing macrolactam polyketides. , Previously, we determined the structure of IdnL7 in complex with an l -alanyl adenylate intermediate analog, 5′- O -[ N -( l -alanyl)­sulfamoyl]­adenosine ( l -alanyl-SA), in the adenylate-forming conformation . The IdnL7 structure clearly revealed the recognition mechanism of the l -alanyl group.…”

“…9,10 The VinM-type adenylation enzyme IdnL7 catalyzes the formation of an amide bond between 3-aminobutyryl-CP and L-alanine in incednine biosynthesis. 9 The VinM-type adenylation enzyme HitE similarly catalyzes the formation of an amide bond between β-phenylalanyl-CP and L-alanine in hitachimycin biosyn- thesis. 10 Thus, amide-forming adenylation enzymes are consistently used in the biosynthesis of β-amino acidcontaining macrolactam polyketides.…”