Functional and Structural Characterization of the Antiphagocytic Properties of a Novel Transglutaminase from Streptococcus suis

Yu, Jie; Pian, Yaya; Ge, Jingpeng; Guo, Jie; Zheng, Yinan; Jiang, Hua; Hao, Huaijie; Yuan, Yuan; Jiang, Yongqiang; Yang, Maojun

doi:10.1074/jbc.m115.643338

Cited by 25 publications

(20 citation statements)

References 47 publications

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“…Fittipaldi and others ( 97 ) reviewed more than 70 virulent factors in the zoonotic agent Streptococcus suis . Interestingly, the enzyme microbial transglutaminase, which is extensively used in the processed food industries ( 98 – 100 ), has been recently described as a virulence factor in this bacterium ( 101 ). Finally, the resistome present in probiotics is most probably underestimated and the double-edged sword effects of probiotics such as health benefits vs antibiotic resistance gene dissemination to the gut microbiome have to be carefully contemplated ( 102 ).…”

Section: Hgt Potential Of Probioticsmentioning

confidence: 99%

“…They have been demonstrated recently as immunogenic in celiac disease patients, representing a new marker that reflects the intestinal damage ( 100 ). In pathogens such as S. suis , the enzyme is a virulence factor, conferring antiphagocytic properties ( 101 ). It is not clear whether microbial transglutaminases in other bacteria may play a role in virulence.…”

Section: Industrially Processed Foodmentioning

confidence: 99%

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Potential Effects of Horizontal Gene Exchange in the Human Gut

Tenbusch²,

2017

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Many essential functions of the human body are dependent on the symbiotic microbiota, which is present at especially high numbers and diversity in the gut. This intricate host–microbe relationship is a result of the long-term coevolution between the two. While the inheritance of mutational changes in the host evolution is almost exclusively vertical, the main mechanism of bacterial evolution is horizontal gene exchange. The gut conditions, with stable temperature, continuous food supply, constant physicochemical conditions, extremely high concentration of microbial cells and phages, and plenty of opportunities for conjugation on the surfaces of food particles and host tissues, represent one of the most favorable ecological niches for horizontal gene exchange. Thus, the gut microbial system genetically is very dynamic and capable of rapid response, at the genetic level, to selection, for example, by antibiotics. There are many other factors to which the microbiota may dynamically respond including lifestyle, therapy, diet, refined food, food additives, consumption of pre- and probiotics, and many others. The impact of the changing selective pressures on gut microbiota, however, is poorly understood. Presumably, the gut microbiome responds to these changes by genetic restructuring of gut populations, driven mainly via horizontal gene exchange. Thus, our main goal is to reveal the role played by horizontal gene exchange in the changing landscape of the gastrointestinal microbiome and potential effect of these changes on human health in general and autoimmune diseases in particular.

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Section: Hgt Potential Of Probioticsmentioning

confidence: 99%

Section: Industrially Processed Foodmentioning

confidence: 99%

Potential Effects of Horizontal Gene Exchange in the Human Gut

Tenbusch²,

2017

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“…After establishing immunogenicity in CD, its actual potential pathogenicity is being currently unraveled. In this sense, mTG was recently shown to exert an anti‐phagocytic effect , thus suppressing a crucial component of human immunity. Furthermore, it can be internalized trans‐enterocytically to be deposited beneath the human enteric epithelium, thus facing the immune cells and systems .…”

Section: Discussionmentioning

confidence: 99%

Antibodies against neo-epitope of microbial and human transglutaminase complexes as biomarkers of childhood celiac disease

Agardh

Tenbusch²,

Wusterhausen³

et al. 2019

Clinical and Experimental Immunology

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Summary Tissue transglutaminase (tTG) and microbial transglutaminase (mTG) cross‐link gliadins to form complexes that expose immunogenic neo‐epitopes to produce tTG and mTG‐neo‐epitope antibodies. The aim of this study was to test the diagnostic performance of antibodies against non‐complexed and complexed forms of transglutaminases, to correlate their activities to the intestinal damage and to explore age group dependency in celiac disease (CD). A total of 296 children with untreated CD and 215 non‐celiac disease controls were checked by in‐house enzyme‐linked immunosorbent assays detecting immunoglobulin (Ig)A, IgG or combined detection of IgA and IgG (check) against tTG, AESKULISA® tTG New Generation (tTG‐neo) and mTG‐neo (RUO), IgA and IgG antibodies against deamidated gliadin peptide (DGP) and human IgA anti‐endomysium antibodies (EMA) using AESKUSLIDES® EMA. Intestinal pathology was graded according the revised Marsh criteria, and age dependencies of the antibody activities were analysed. Using cut‐offs estimated from receiver operating characteristic (ROC) curves, the highest area under curve (AUC) of the TG assays was 0·963 for tTG‐neo check, followed by tTG check (0·962) when the diagnosis was based on enteric mucosal histology. tTG‐neo check was the most effective to reflect the intestinal abnormalities in CD (r = 0·795, P < 0·0001). High levels of anti‐mTG‐neo IgG and anti‐tTG‐neo IgG appeared in the earlier age groups, as compared to anti‐tTG IgG (P < 0·001). Considering antibody diagnostic performance based on AUC, enteric damage reflection and predictability at an early age, the anti‐neo tTG check was the most effective diagnostic biomarker for pediatric CD. The mTG neo check might represent a new marker for CD screening, diagnosis and predictability.

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“…glutaminase has yet to be formally reported. [30,31] In addition, a collection of recently identified bacteria have been shown to possess the capacity to secrete transglutaminase at high yields. [18,19] It is hoped that the use of these strains will enable a reduction in the cost of the manufacture of high purity transglutaminases due to decreased requirement for downstream processing.…”

Section: Enzymementioning

confidence: 99%

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

et al. 2020

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Intermolecular cross-linking is one of the most important techniques that can be used to fundamentally alter the material properties of a polymer. The introduction of covalent bonds between individual polymer chains creates 3D macromolecular assemblies with enhanced mechanical properties and greater chemical or thermal tolerances. In contrast to many chemical cross-linking reactions, which are the basis of thermoset plastics, enzyme catalysed processes offer a complimentary paradigm for the assembly of cross-linked polymer networks through their predictability and high levels of control. Additionally, enzyme catalysed reactions offer an inherently 'greener' and more biocompatible approach to covalent bond formation, which could include the use of aqueous solvents, ambient temperatures, and heavy metal-free reagents. Here, we review recent progress in the development of biocatalytic methods for polymer cross-linking, with a specific focus on the most promising candidate enzyme classes and their underlying catalytic mechanisms. We also provide exemplars of the use of enzyme catalysed cross-linking reactions in industrially relevant applications, noting the limitations of these approaches and outlining strategies to mitigate reported deficiencies.

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Functional and Structural Characterization of the Antiphagocytic Properties of a Novel Transglutaminase from Streptococcus suis

Cited by 25 publications

References 47 publications

Potential Effects of Horizontal Gene Exchange in the Human Gut

Potential Effects of Horizontal Gene Exchange in the Human Gut

Antibodies against neo-epitope of microbial and human transglutaminase complexes as biomarkers of childhood celiac disease

Enzyme‐catalysed polymer cross‐linking: Biocatalytic tools for chemical biology, materials science and beyond

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