Functional and Structural Analysis of the Siderophore Synthetase AsbB through Reconstitution of the Petrobactin Biosynthetic Pathway from Bacillus anthracis

Nusca, Tyler D.; Kim, Young Chang; Maltseva, N.; Lee, Jung Yeop; Eschenfeldt, William H.; Stols, Lucy; Schofield, Michael M.; Scaglione, Jamie B.; Dixon, Shandee D.; Oves-Costales, Daniel; Challis, Gregory L.; Hanna, Philip C.; Pfleger, Brian F.; Joachimiak, A.; Sherman, David H.

doi:10.1074/jbc.m112.359349

Cited by 31 publications

(73 citation statements)

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“…In the siderophore oriented sequences, 46% of the sequence showed similarity with the siderophore assembly subunit i.e. siderophore synthetase AsbS [26]; 27% of the sequence showed best hit classification with pyoverdine, which are generally the fluorescent siderophore produced by the members of the pseudomonaceae family like Azotobacter , Azomonas , Pseudomonas and Rhizobacter [27]; 13% were for siderophore regulating receptor system and rest 15% showed identities with the yersiniabactin (siderophore produced by the pathogenic bacteria Yersinia pestis , Yersinia pseudotuberculosis and Yersininaenterocolitica ) [28]; aerobactin (siderophore produced by E . coli ) [29]; bacillibactin (siderophore produced by the genus Bacillus ) [30]; achromobactin (siderophore produced by Pseudomonas syringe ) [31]; enterobactin and pyochelin (siderophore produced by Pseudomonas aeruginosa ) [32] respectively.…”

Section: Resultsmentioning

confidence: 99%

A metagenomic approach to decipher the indigenous microbial communities of arsenic contaminated groundwater of Assam

et al. 2017

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Metagenomic approach was used to understand the structural and functional diversity present in arsenic contaminated groundwater of the Ganges Brahmaputra Delta aquifer system. A metagene dataset (coded as TTGW1) of 89,171 sequences (totaling 125,449,864 base pairs) with an average length of 1406 bps was annotated. About 74,478 sequences containing 101,948 predicted protein coding regions passed the quality control. Taxonomical classification revealed abundance of bacteria that accounted for 98.3% of the microbial population of the metagenome. Eukaryota had an abundance of 1.1% followed by archea that showed 0.4% abundance. In phylum based classification, Proteobacteria was dominant (62.6%) followed by Bacteroidetes (11.7%), Planctomycetes (7.7%), Verrucomicrobia (5.6%), Actinobacteria (3.7%) and Firmicutes (1.9%). The Clusters of Orthologous Groups (COGs) analysis indicated that the protein regulating the metabolic functions constituted a high percentage (18,199 reads; 39.3%) of the whole metagenome followed by the proteins regulating the cellular processes (22.3%). About 0.07% sequences of the whole metagenome were related to genes coding for arsenic resistant mechanisms. Nearly 50% sequences of these coded for the arsenate reductase enzyme (EC. 1.20.4.1), the dominant enzyme of ars operon. Proteins associated with iron acquisition and metabolism were coded by 2% of the metagenome as revealed through SEED analysis. Our study reveals the microbial diversity and provides an insight into the functional aspect of the genes that might play crucial role in arsenic geocycle in contaminated ground water of Assam.

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Section: Resultsmentioning

confidence: 99%

A metagenomic approach to decipher the indigenous microbial communities of arsenic contaminated groundwater of Assam

et al. 2017

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“…It is found that the function of AsbA synthase can be compensated by AsbB. The strain deletion of AsbA synthetase with the other five genes can also synthesize part of Petrobactin (Nusca et al, 2012). The asbE gene deletion cannot synthesize Petrobactin siderophore showed that asbE gene was also a key gene in the pathway of Petrobactin siderophore synthesis.…”

Section: Resultsmentioning

confidence: 99%

“…Petrobactin is the main siderophore in Bacillus anthracis. Because it is not identified by the innate immune system and it has been found that the Petrobactin is the cardinal factor in the anthrax model, most of which is the mixed type of catechol-carboxylate siderophore (Nusca et al, 2012). It appears that Bacillibactin has higher importance in Bacillus cereus on insect model experiments (Hayrapetyan et al, 2016).…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, the pathogenicity of asbA deletion mutant mice was also significantly reduced (Cendrowski et al, 2004). Nusca et al (2012) realized the heterologous expression of Petrobactin siderophore by using citric acid, spermidine and 3, 4-two hydroxybenzoic acid substrates. It is found that the function of AsbA synthase can be compensated by AsbB.…”

Section: Resultsmentioning

confidence: 99%

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Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

Sheng¹,

Jia²,

Xiaomai³

et al. 2018

American Journal of Biochemistry and Biotechnology

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Objective of this paper is to excavate the siderophore synthesis gene from Brevibacillus brevis GZDF3 and verify its type and antibacterial effects. The method is using genome mining technology to analyze the siderophore synthesis genes and the phylogenetic tree of each synthesis gene was constructed separately. Iron free medium was utilized to induce the synthesis of siderophore and CAS liquid detection method was used for qualitative and quantitative analysis on siderophore. The type of siderophore was preliminaries identified by Arnow and its antibacterial effects were analyzed according to the agar punching method. The results show that a siderophore synthesis gene cluster with 83% similarity to Petrobactin was found in Brevibacillus brevis GZDF3 genome. Iron free medium could induce siderophore synthesis and the optimal incubation time cultured in iron free medium was 30 h and 48 h. Antagonistic strain GZDF3 had the capacity to synthesize catechol-type siderophore. Also, GZDF3 had a powerful antibacterial effect on pathogenic fungus Fusarium oxysporum of rotted root on Pinellia ternata. Therefore, Brevibacillus brevis GZDF3 can produce catechol-type siderophore in an iron-deficient culture medium, which was also a main antifungal active substance.

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“…The presence of 4,4 0diaminodibutylamine groups rather than spermidines in rhodopetrobactins can be explained by the substrate specificity of AsbA and AsbB. Both synthetases can be active with a range of diamine substrates, producing petrobactin analogues differing only in the polyamine linker groups (Nusca et al, 2012).…”

Section: Genomics Of Rhodopetrobactin Productionmentioning

confidence: 99%

The purple non‐sulfur bacterium Rhodopseudomonas palustris produces novel petrobactin‐related siderophores under aerobic and anaerobic conditions

Baars

Morel

Zhang

2018

Environmental Microbiology

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Many bacteria produce siderophores to bind and take up Fe(III), an essential trace metal with extremely low solubility in oxygenated environments at circumneutral pH. The purple non-sulfur bacterium Rhodopseudomonas palustris str. CGA009 is a metabolically versatile model organism with high iron requirements that is able to grow under aerobic and anaerobic conditions. Siderophore biosynthesis has been predicted by genomic analysis, however, siderophore structures were not identified. Here, we elucidate the structure of two novel siderophores from R. palustris: rhodopetrobactin A and B. Rhodopetrobactins are structural analogues of the known siderophore petrobactin in which the Fe chelating moieties are conserved, including two 3,4-dihydroxybenzoate and a citrate substructure. In the place of two spermidine linker groups in petrobactin, rhodopetrobactins contain two 4,4'-diaminodibutylamine groups of which one or both are acetylated at the central amine. We analyse siderophore production under different growth modes and show that rhodopetrobactins are produced in response to Fe limitation under aerobic as well as under anaerobic conditions. Evaluation of the chemical characteristics of rhodopetrobactins indicates that they are well suited to support Fe acquisition under variable oxygen and light conditions.

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Functional and Structural Analysis of the Siderophore Synthetase AsbB through Reconstitution of the Petrobactin Biosynthetic Pathway from Bacillus anthracis

Cited by 31 publications

References 73 publications

A metagenomic approach to decipher the indigenous microbial communities of arsenic contaminated groundwater of Assam

A metagenomic approach to decipher the indigenous microbial communities of arsenic contaminated groundwater of Assam

Mining, Isolation and Identification of Siderophore Synthesis Gene from Brevibacillus brevis GZDF3

The purple non‐sulfur bacterium Rhodopseudomonas palustris produces novel petrobactin‐related siderophores under aerobic and anaerobic conditions

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