Functional and Safety Aspects of Enterococci Isolated from Different Spanish Foods

Omar, Nabil Ben; Castro, Araceli; Lucas, Rosario; Abriouel, Hikmate; Yousif, N.M.K.; Franz, Charles M. A. P.; Holzapfel, Wilhelm H.; Pulido, Rubén Pérez; Martı́nez-Cañamero, Magdalena; Gálvez, Antonio

doi:10.1078/0723-2020-00248

Cited by 186 publications

(64 citation statements)

References 51 publications

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“…Isolates have been identified, characterized, and utilized with Lactobacillus pentosus as starter cultures for Spanish-style green olive fermentation. In another study [10], three strains of Enterococci were isolated from olives, belonged to the species E. faecium. Furthermore, a total number of 52 LAB have been isolated from naturally fermented green olives collected from different areas of Sicily [11].…”

Section: Isolation and Identification Of Labmentioning

confidence: 99%

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

2018

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Table olive is one of the main fermented vegetable worldwide and can be processed as treated or natural product. Lactic Acid Bacteria (LAB) are responsible for the fermentation of treated olives. The aim of this work was to study the technological characteristics and the potential probiotic properties of LAB isolated from Cypriot green table olives. This is the first comprehensive report on the isolation and characterization of LAB isolates retrieved from Cypriot green table olives. From a collection of 92 isolates from spontaneously fermenting green olives, 64 g positive isolates were firstly identified to genus level using biochemical tests, and secondly to species level using multiplex species specific polymerase chain reaction (PCR) amplifications of the sodA gene. Moreover, each of our isolates were tested for their technological and probiotics properties, as well as for their safety characteristics, using biochemical and molecular methods, in order to be used as starter cultures. Finally, to discriminate the most promising isolates on the base of their technological and probiotics properties, Principal component analysis was used. All the isolates were identified as Enteroccocus faecium, having interesting technological properties, while pathogenicity determinants were absent. Principal component analysis showed that some isolates had a combination of the tested parameters. These findings demonstrate that enteroccoci from Cypriot table olives should be considered as a new source of potential starter cultures for fermented products, having possibly promising technological and probiotic attributes.

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Section: Isolation and Identification Of Labmentioning

confidence: 99%

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

2018

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“…Similar results reported previously [10,15] . GÜLHAN, BOYNUKARA, ÇİFTCİ ÜNLÜ SÖĞÜT, FINDIK The genotypic diversity of enterococcal isolates obtained from different origins was assessed using RAPD-PCR fingerprinting [10,11,17] . The RAPD-PCR analysis classified five profiles were discerned for E. faecium [11] .…”

Section: Discussionmentioning

confidence: 99%

Köpek, Kedi ve İnsan Orijinli Enterococcus feacium İzolatlarının Biofilm Üretimi, Genotip ve Antibiyotik Direnç Profillerinin Belirlenmesi

Gülhan¹,

Boynukara²,

Çiftci³

et al. 2015

Kafkas Univ Vet Fak Derg

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The aim of the study was to determine the biofilm production, genotypes, antibiotics resistance patterns and antibiotypes of 82 Enterococcus faecium strains isolated from dog, cat and human. Of examined strains biofilm production detected totally 72 (87.8%) in 35 (97.2%) dog, 22 (78.6%) cat and 15 (83.3%) human isolates. Genotyping of isolates was performed by RAPD-PCR and 16, 3 and 4 different profiles were detected in dog, cat and human isolates, respectively. In total of 98.8% with a maximum resistance to nalidixic acid and 4.9% with the lowest resistance to vancomycin was found. None of vancomycin resistance 4 isolates, vancomycin resistance genes (vanA, vanB, vanC1/C2 or vanD) has been detected. Antibiotyping of isolates was performed with UPGMA and 5 groups of dog, 10 groups of cat and 7 groups of human isolates were determined. The results from this study indicate that healthy dogs and cats are a source of Antibiotic resistant enterococci and may act as a reservoir of resistance that can be transferred from pets to people. Also our results demonstrated that the phenotype and genotype patterns found among enterococci strains from dogs, cats and humans were heterogeneous. (vanA, vanB, vanC1/2 veya vanD) saptanamadı. UPGMA ile yapılan antibiyotiplendirmede köpek izolatları 5, kedi izolatları 10 ve insan izolatları da 7 grup altında toplandı. Çalışma sonuçları sağlıklı köpek ve kedilerin antibiyotiklere dirençli enterokoklar için kaynak oluşturabileceğini ve insanlara direncin aktarılmasında rezervuar olarak rol oynayabileceğini göstermiştir. Ayrıca, elde edilen sonuçlarla köpek, kedi ve insanlar orijinli enterokoklarda bulunan fenotipik ve genotipik patternlerin heterojen olduğu belirlenmiştir. Keywords

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“…The following tests were made: gelatinase, lipase, hemolytic activity and DNAse [15][16][17][18]. The formation of a transparent or opalescent halo indicated test positivity.…”

Section: Phenotypic Virulence Testsmentioning

confidence: 99%

Antagonistic Activity, Antimicrobial Susceptibility and Potential Virulence Factors of Enterococcus faecalis

Carneiro¹,

Evangelista-Barreto²,

Silveira-Oliveira³

et al. 2015

JLS

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Enterococcus faecalis isolates (87) were phenotypically and genotypically identified and subsequently subjected to the antagonism test and antimicrobial susceptibility. The lipolitic, hemolytic and DNAse activities were identified along with the genes gelE, cylL, cylS, ccf, cpd and cob that, encode virulence determinants. Thirty seven percent of isolates inhibited Listeria monocytogenes (CERELA), Listeria innocuous (CERELA), Staphylococcus aureus (ATCC25932), Lactococcus lactis (IL1403), Micrococcus luteus (ATCC10240) and Enterococcus faecalis (ATCC29212). All strains were sensitive to the ampicillin antibiotic, but 47% were resistant to at least one antimicrobial agent and 6% of isolates presented multidrug resistance. Ninety seven percent of isolates contained the gelE gene, but 77% of these isolates showed gelatinase activity. Presence of cylL and cylS genes was observed in 25% of the isolates, but only 5% presented hemolytic activity. None isolates showed lipase and DNAse activities. Eight percent of isolates contained the ccf gene and 2% showed the presence of the cpd and cob genes. The ability to inhibit pathogenic bacteria, low resistance to antibiotics and absence of virulence factors make some of Enterococcus faecalis strains characterized in the present study promising for exploitation in other applications such as probiotics in aquaculture.

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Functional and Safety Aspects of Enterococci Isolated from Different Spanish Foods

Cited by 186 publications

References 51 publications

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

Enterococci Isolated from Cypriot Green Table Olives as a New Source of Technological and Probiotic Properties

Köpek, Kedi ve İnsan Orijinli Enterococcus feacium İzolatlarının Biofilm Üretimi, Genotip ve Antibiyotik Direnç Profillerinin Belirlenmesi

Antagonistic Activity, Antimicrobial Susceptibility and Potential Virulence Factors of Enterococcus faecalis

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