Functional and Morphological Evaluation of the Mucosa of Endometrium and Oviduct in Postpartum Goats

Abstract

The aim of this work was to characterize the structural changes in the surface epithelium and endometrial glands as well as oviduct mucosa in goats after parturition using the light and transmission electron microscopy. Fifteen Slovak short-haired goats were used. They were killed on days 3, 21, 36, and 40 after parturition. The evaluation of semithin sections from day 3 postpartum revealed impaired epithelial cells. Lymphocytes and neutrophil granulocytes were present in the lumen of uterine glands. Ciliated cells and sporadic secretory cells that were released into the oviduct lumen were found on semithin sections from the ampullary part of oviducts (day 3 postpartum). Cells in the cytoplasm contained mitochondria and cisternae of endoplasmic reticulum. Impaired cells with a vacuolised cytoplasm were present on ultrathin sections from the caruncular region of endometrium on day 21 postpartum. Uterine glands were in the stage of proliferation. Ultrathin sections from day 21 postpartum showed that ciliated cells were present and secretory cells with secretory granules increased in the epithelium of oviduct ampulla. The surface epithelium on day 36 was continuous, simple columnar; in some locations pseudostratified columnar. No signs of morphological damage of cells were found. On the free border of ciliated cells of the oviduct mucosa on day 36, the presence of numerous kinocilia was observed. Glandular cells contained a great amount of dense secretory granules in the cytoplasm (day 36 postpartum). The microscopic and submicroscopic appearance of the uterus and oviduct on day 40 postpartum was similar to that on day 36 of the period observed. The results obtained can be used in synchronization or induction of estrus in the intensification of reproduction and increasing the reproductive turnover in goat husbandry. , uterus, oviduct, puerperium, light and electron microscopy Goat

Activity of alkaline and acidic phosphatase in glandular cells of uterine endometrium of puerperal ewes after exposure to polychlorinated biphenyls

“…The range of values of its activity showed significant difference (P<0.001) between day 17 and day 34 of the evaluated period in the control group. Similar results were reported by Krajni~áková et al, (2002) in regressive changes occurring in the uterus during the postpartum period of goats. Zamiri et Blackshaw (1979) claim that alkaline phosphatase activity at the surface uterine glands of ewes gradually increased with peak values between day 14 and day 21 of the estral cycle.…”

“…Zamiri et Blackshaw (1979) claim that alkaline phosphatase activity at the surface uterine glands of ewes gradually increased with peak values between day 14 and day 21 of the estral cycle. The proliferation process of endometrial structures of sheep and goats as observed by Krajni~áková et al, (1999Krajni~áková et al, ( , 2002 is consistent with the occurrence of alkaline phosphatase activity and connected with their role in transformation mechanisms and metabolic processes occurring in the postpartum uterus. Statistical significance compared to day 17, 25 and 34 days after parturition ** = P<0.01; *** = P<0.001 Significance of differences between the control and experimental groups during the observed days after parturition a : a = P>0.05 ; b : b = P< 0.001 No significant statistical difference was noted (P>0.05) when evaluating alkaline phosphatase activity in the glandular cells of the endometrium (Tab.…”

“…The rate of the acidic phosphatase reaction begins to increase significantly as early as the onset of the follicular phase (Uhrín, 1992) of the estrous cycle in cows. An increasing trend of alkaline and acidic phosphatase activity with the progressing of the postpartal period in goats was reported by Krajni~áková et al (2002). Marjanov et al (1985) observed that the activity of alkaline phosphatase decreased in the endometrium of sows after administration of medrooxyprogesterone.…”

Activity of alkaline phosphatase, acidic phosphatase and nonspecific esterase in the oviducts of puerperal ewes after exposure to polychlorinated biphenyls

“…Similar results were reported by Krajnicakova et al (2002) in regressive changes occurring in the NsE activity is given as the integrated absorbance in min/µm 3 of oviductal endothelial cells at a wavelength of 540 nm; *P < 0.05, **P < 0.01. Statistical significance compared between Day 17 and other days after parturition; b P < 0.001.…”

The distribution and immunolocalization of fibroblast growth factors (FGFs) in the rat oviduct during early pregnancy and the post‐partum period

“…In healthy animals, the secretory activity of the oviduct decreases during the luteal phase and pregnancy, both of which are characterized by high progesterone levels (Nayak et al., 1976). In a study conducted in the oviduct of goats in the post‐partum period when the effect of the progesterone hormone disappears, it is reported that the number of secretory cells containing a large number of secretory granules in the ampulla epithelium increases from the post‐partum 21st day (Krajničáková et al., 2002). In another study examining the morphological features of the sheep oviduct in the post‐partum period, it is stated that the secretory cells in the early post‐partum period are light coloured and do not contain secretory granules.…”