Functional and morphological analysis of isolates of phylloplane and rhizoplane endophytic bacteria interacting in different cocoa production systems in the Amazon

Júnior, Miguel Alves; Sousa, Fabiana Oliveira de; Silva, Thays Ferreira; Albino, Ulisses Brigatto; Garcia, Magali Gonçalves; Moreira, Simone Maria Costa de Oliveira; Vieira, Marcos Ribeiro da Silva

doi:10.1016/j.crmicr.2021.100039

Cited by 3 publications

(1 citation statement)

References 42 publications

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“…The phyllosphere is the habitat for a large diversity of microorganisms. Amongst them, a group of endophytic bacteria and fungi may colonize the internal tissues of host plants (Alves-Junior et al, 2021). These microbes can be isolated from flowers, fruits, leaves, stems, roots, and seeds of various plant species (Qin et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Endophytes Against Bacterial Blight of Pomegranate

Karn¹,

Sharma²,

Sharma³

et al. 2022

IJBSM

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The endophytic study was undertaken during 2019–2020 at department of plant pathology, Dr, YS Parmar UHF, Nauni, Solan (HP), India against Xanthomonas axonopodis pv. punicae inciting bacterial blight of pomegranate. Endophytes which we used, either endophytic bacteria or fungi have been isolated from four different genotypes/cultivars (Wild pomegranate, Kandhari, G-137 and Sindhuri) of pomegranate to study the isolation procedure and characterization of the endophytic microbes that colonize pomegranate plants. The huge diversity among the endophytic microbes and the host plants hinders a uniform protocol for isolation of endophytes and the establishment of inciting agent of bacterial blight. The most common technique that is currently used involves a thorough surface sterilization followed by fragmentation of the plant tissue and dilutions, culture of the fragments onto agar plates for endophytic bacteria and fragmentation of leaf bits after thorough surface sterilization onto agar plate for endophytic fungi. Among 27 and 19 bacterial and fungal endophytes, three potential microbes of each were selected after in vitro assay against Xanthomonas axonopodis pv. punicae which causes bacterial blight disease, and were identified based on morphological, biochemical, and cultural characteristics, respectively. This is the first report of endophytic microbes from the leaves of Punica granatum genotypes/cultivars in Himachal Pradesh, India. Future studies will determine the potential application of these isolates in biological control, growth promotion, enzyme production and colonization in host for induction of defense mechanism against bacterial blight disease of pomegranate.

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Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Endophytes Against Bacterial Blight of Pomegranate

Karn¹,

Sharma²,

Sharma³

et al. 2022

IJBSM

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Characterization of the microbiota dynamics associated with Moniliophthora roreri, causal agent of cocoa frosty pod rot disease, reveals new viral species

et al. 2023

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IntroductionTheobroma cacao, the cocoa tree, is a target for pathogens, such as fungi from the genera Phytophthora, Moniliophthora, Colletotrichum, Ceratocystis, among others. Some cacao pathogens are restricted to specific regions of the world, such as the Cacao swollen shoot virus (CSSV) in West African countries, while others are expanding geographically, such as Moniliophthora roreri in the Americas. M. roreri is one of the most threatening cacao pathogens since it directly attacks the cacao pods driving a significant reduction in production, and therefore economic losses. Despite its importance, the knowledge about the microenvironment of this pathogen and the cocoa pods is still poorly characterized.MethodsHerein we performed RNA sequencing of spores in differential stages of culture in a medium supplemented with cacao pod extract and mycelium collected of the susceptible variety ICT 7121 naturally infected by the pathogen to evaluate the diversity and transcriptional activity of microorganisms associated with the in vitro sporulation of M. roreri.ResultsOur data revealed a great variety of fungi and bacteria associated with M. roreri, with an exceptional diversity of individuals from the genus Trichoderma sp. Interestingly, the dynamics of microorganisms from different kingdoms varied proportionally, suggesting they are somehow affected by M. roreri culture time. We also identified three sequences similar to viral genomes from the Narnaviridae family, posteriorly confirmed by phylogenetic analysis as members of the genus Narnavirus. Screening of M. roreri public datasets indicated the virus sequences circulating in samples from Ecuador, suggesting a wide spread of these elements. Of note, we did not identify traces of the viral sequences in the M. roreri genome or DNA sequencing, restricting the possibility of these sequences representing endogenized elements.DiscussionTo the best of our knowledge, this is the first report of viruses infecting the fungus of the genus Moniliophthora and only the third description of viruses that are able to parasite elements from the Marasmiaceae family.

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Diversity Analysis and Function Prediction of Bacterial Communities in the Different Colored Pericarp of Citrus reticulata cv. ‘Shatangju’ Due to ‘Candidatus Liberibacter asiaticus’ Infection

Wang

Zhu

Zhang

et al. 2023

IJMS

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Huanglongbing (HLB), caused by the Candidatus Liberibacter spp., is the most devastating disease in the citrus industry. HLB significantly affects and alters the microbial community structure or potential function of the microbial community of leaves and roots. However, it is unknown how the microbial community structure of the pericarp with different pigments is affected by Candidatus Liberibacter asiaticus (CLas). This study identified the enriched taxa of the microbial community in the citrus pericarp with normal or abnormal pigment and determine the effects of HLB on the pericarp microbial community using 16S rRNA-seq. The alpha and beta diversity and composition of microbial communities were significantly different between normal and abnormal pigment pericarp tissues of ripe fruits infected by CLas. Firmicutes, Actinobacteriota, Bacteroidota, Acidobacteriota, and Desulfobacterota dominated the pericarp microbiota composition in WDYFs (whole dark yellow fruits) samples. The relative abundance of most genera in WDYFs was higher than 1%, such as Burkholderia, and Pelomonas. However, with the exception of the HLB pathogen, the relative abundance of most genera in the abnormal-colored pericarp samples was less than 1%. CLas decreased the relative abundance of pericarp taxonomic. The predicted function of microbial was more plentiful and functional properties in the WDYF sample, such as translation, ribosomal structure and biogenesis, amino acid transport and metabolism, energy production and conversion, and some other clusters of orthologous groups (COG) except for cell motility. The results of this study offer novel insights into understanding the composition of microbial communities of the CLas-affected citrus pericarps and contribute to the development of biological control strategies for citrus against Huanglongbing.

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Functional and morphological analysis of isolates of phylloplane and rhizoplane endophytic bacteria interacting in different cocoa production systems in the Amazon

Cited by 3 publications

References 42 publications

Isolation and Characterization of Endophytes Against Bacterial Blight of Pomegranate

Isolation and Characterization of Endophytes Against Bacterial Blight of Pomegranate

Characterization of the microbiota dynamics associated with Moniliophthora roreri, causal agent of cocoa frosty pod rot disease, reveals new viral species

Diversity Analysis and Function Prediction of Bacterial Communities in the Different Colored Pericarp of Citrus reticulata cv. ‘Shatangju’ Due to ‘Candidatus Liberibacter asiaticus’ Infection

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