Functional and molecular immune response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria

Costa, María M.; Prado-Álvarez, María; Gestal, Camino; Li, H.; Roch, Philippe; Novoa, Beatriz; Figueras, António

doi:10.1016/j.fsi.2009.02.001

Cited by 123 publications

(82 citation statements)

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“…Similarly, the temporal control of NF-jB activation in mammals is coordinated by degradation and synthesis of IjB proteins which are responsible for the strong negative regulation allowing a fast turn-off of the NF-jB response (Hoffmann et al, 2002). On the opposite, up-and down-regulation of the various AMP genes in M. galloprovincialis was reported by several laboratories using different techniques (Costa et al, 2009b;Li et al, 2010;Mitta et al, 2000a;Pallavicini et al, 2008;Venier et al, 2011) and appears somehow conflicting. Details related to the experimental challenges (e.g.…”

Section: Tablementioning

confidence: 99%

Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis

Toubiana

Rosani

Giambelluca

et al. 2014

Developmental & Comparative Immunology

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a b s t r a c tBased on protein domain structure and organization deduced from mRNA contigs, 15 transcripts of the Toll signaling pathway have been identified in the bivalve, Mytilus galloprovincialis. Identical searches performed on publicly available Mytilus edulis ESTs revealed 11 transcripts, whereas searches performed in genomic and new transcriptome sequences of the Pacific oyster, Crassostrea gigas, identified 21 Toll-related transcripts. The remarkable molecular diversity of TRAF and IKK coding sequences of C. gigas, suggests that the sequence data inferred from Mytilus cDNAs may not be exhaustive. Most of the Toll pathway genes were constitutively and ubiquitously expressed in M. galloprovincialis, although at different levels, and clearly induced after in vivo injection with bacteria. Such over-transcription was more rapid and intense with Gram-negative than with Gram-positive bacteria. Injection of a fungus modulated the transcription of few Toll pathway genes, with the induction levels of TLR/MyD88 complex being always less intense. Purified LPS and b-glucans had marginal effect whereas peptidoglycans were ineffective. At the moment, we found no evidence of an IMD transcript in bivalves. In conclusion, mussels possess a complete Toll pathway which can be triggered either by Gram-positive or Gram-negative bacteria.

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Section: Tablementioning

confidence: 99%

Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis

Toubiana

Rosani

Giambelluca

et al. 2014

Developmental & Comparative Immunology

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“…V. anguillarum is one of the most common pathogens to marine bivalves. The immune responses of marine bivalves to V. anguillarum challenges have been extensively investigated at molecular levels [7,10]. Fig.…”

Section: Effects Of M Luteus and V Anguillarum On The Proteome Of Hmentioning

confidence: 99%

“…M. galloprovincialis is a preferable bioindicator for marine environmental contaminants in 'Mussel Watch Program' and frequently investigated as an experimental species in ecotoxicology due to its high accumulation and tolerance to marine environmental contaminants [5,6]. In addition, as a filter-feeder, M. galloprovincialis can accumulate a large amount of bacteria from the seawater and therefore has been used in immunity studies of marine aquaculture animals [7]. Traditional approaches in the immune innate system in M. galloprovincialis consist of histological studies on the lesions and the identification and characterization of a certain class of molecules in the immune network at gene and protein levels [7][8][9][10].…”

Section: Introductionmentioning

confidence: 99%

“…In addition, as a filter-feeder, M. galloprovincialis can accumulate a large amount of bacteria from the seawater and therefore has been used in immunity studies of marine aquaculture animals [7]. Traditional approaches in the immune innate system in M. galloprovincialis consist of histological studies on the lesions and the identification and characterization of a certain class of molecules in the immune network at gene and protein levels [7][8][9][10]. For example, infection of vibrios (Vibrio splendidus and Vibrio anguillarum) could significantly up-regulated the expression of antioxidant enzymes (catalase and glutathione transferase), lysozymes and metallothioneins which are involved in immune defense systems in M. galloprovincialis [8].…”

Section: Introductionmentioning

confidence: 99%

“…Micrococcus luteus is a Gram-positive bacterium that can be found in both aquatic and soil environments as well as in aquatic organisms [24]. In M. galloprovincialis, several studies have compared the responses to both Grampositive and Gram-negative bacteria based on the gene expression and functional parameters involved in immune system [7,10]. In this study, the proteomic and metabolomic responses were compared in M. galloprovincialis challenged by V. anguillarum and M. luteus, respectively.…”

Section: Introductionmentioning

confidence: 99%

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Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum

Wei

et al. 2013

Journal of Proteomics

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The outbreak of pathogens can induce diseases and lead to massive mortalities of aquaculture animals including fish, mollusk and shrimp. In this work, the responses induced by Micrococcus luteus and Vibrio anguillarum were investigated in hepatopancreas of mussel Mytilus galloprovincialis using proteomics and metabolomics. Metabolic biomarkers demonstrated that M. luteus and V. anguillarum injections could induce osmotic stress and disturbance in energy metabolism. And the uniquely and more markedly altered metabolic biomarkers (glutamine, succinate, aspartate, glucose, ATP, homarine and tyrosine) indicated that V. anguillarum could cause more severe disturbances in osmotic regulation and energy metabolism. The differentially altered proteins meant that M. luteus and V. anguillarum induced different effects in mussels. However, the common proteomic biomarkers, arginine kinase and small heat shock protein, demonstrated that these two bacteria induced similar effects including oxidative stress and disturbance in energy metabolism in M. galloprovincialis. In addition, some metabolic biomarkers, ATP and glutamine, were confirmed by related proteins including arginine kinase, ATP synthase, nucleoside diphosphate kinase and glutamine synthetase in bacteria-challenged mussels.This study demonstrated that proteomics and metabolomics could provide an insightful view into the effects of environmental pathogens to the marine mussel M. galloprovincialis. Biological significanceThe outbreak of pathogens can lead to diseases and massive mortalities of aquaculture animals including fish, mollusk and shrimp. The mussel M. galloprovincialis distributes widely along the Bohai coast and is popularly consumed as delicious seafood by local residents. This bivalve has become one of the important species in marine aquaculture industry in China. Therefore a study on pathogen-induced effects is necessary. In the present study, an integrated metabolomic and proteomic approach was used to elucidate the differential effects induced by the representative Gram-positive (M. luteus) and Gram-negative (V. anguillarum) bacteria in M. galloprovincialis.

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Diseases and parasites

Gosling¹

2015

Marine Bivalve Molluscs

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Functional and molecular immune response of Mediterranean mussel (Mytilus galloprovincialis) haemocytes against pathogen-associated molecular patterns and bacteria

Cited by 123 publications

References 50 publications

Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis

Toll signal transduction pathway in bivalves: Complete cds of intermediate elements and related gene transcription levels in hemocytes of immune stimulated Mytilus galloprovincialis

Proteomic and metabolomic responses in hepatopancreas of Mytilus galloprovincialis challenged by Micrococcus luteus and Vibrio anguillarum

Diseases and parasites

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