Functional and molecular consequences of ionizing irradiation on large conductance Ca2+-activated K+ channels in rat aortic smooth muscle cells

Plasmonic gold nanoparticles possess the ability to open potassium channels in rat thoracic aorta smooth muscles in a remote control manner

“…The data obtained clearly indicate that decrease in outward current density in both endothelial and SM cells combined with diminished BK Ca mRNA expression led to vascular hypercontractility and hypertension development [13].…”

“…The underlying mechanisms include impaired vasodilator potential and decreased endothelium-dependent vasorelaxation [5,15], and suppression of ionic currents through potassium channels ( [6,7,13,14]). Finally, all these factors taken together promote vascular malfunction and decreased tissue blood supply.…”

Electrophysiological and contractile evidence of the ability of human mesenchymal stromal cells to correct vascular malfunction in rats after ionizing irradiation

“…channel (BK Ca ) activity in endothelial cells [2]. Nevertheless, although endothelium is the most radiation-sensitive and vulnerable component of the vascular wall, the studies performed have shown that irradiation has the ability to enhance vascular contractility in an endothelium-independent manner [3,15]. Another potential mechanism by which vascular tone could be enhanced is through changes in the transmembrane potential of the vascular SMC or myofilament calcium sensitivity.…”

“…-dependent potassium channels (BK Ca ) activity in endothelial cells seems to be one of the most vulnerable mechanisms of vasodilatation in vascular wall under irradiation [1,2]. It has recently been shown that both BK Ca channels function and expression in smooth muscle cells (SMC) are very sensitive to radiation, suggesting that the vasodilator potential of vascular smooth muscle had decreased following irradiation [3].…”

“…Restrictase EarI was employed in some cases for the verification of PCR products with a single restriction site (expected sizes of both fragments are indicated also). MSCs(1)(2)(3)(4)(5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15)(16), bone marrow progenitors(18)(19)(20): 1 CD45, 2 CD34, 3 CD14, 4 CD71, 5 CD29, 6 CD29/EarI, 7 CD44, 8 CD44/EarI, 9 CD105, 10 CD105/EarI, 11 CD73, 12 CD73/ EarI, 13 CD166, 14 CD166/EarI, 15 CD90, 16 CD90/EarI, 17 50 base-pair ladder, 18 CD45, 19 CD34, Endothelium-dependent acetylcholine-induced vascular responses in rat thoracic aorta rings obtained from healthy (open circles) and irradiated (open squares) rats and hMSC treated nonirradiated (closed circles) and post-irradiated (closed squares) animals. hMSC were administered intravenously on the 7th day after irradiation (6 Gy).…”