Functional and histopathological study of the pancreas in hamsters (Mesocricetus auratus) infected and reinfected with Trypanosoma cruzi

Santos, Vitorino Modesto dos; Lima, Marcus Aurelho de; Cabrine-Santos, Marlene; Marquez, Daniela de Stefani; Pereira, Gilberto de Araújo; Lages‐Silva, Eliane; Ramı́rez, Luı́s Eduardo

doi:10.1007/s00436-004-1183-8

Cited by 13 publications

(21 citation statements)

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“…However, studies in human tongues and the results found for the area of the lumen of the ducts of the glands of von Ebner are the first report of this type in CD patients. The dilatation of the ducts may be ascribed to the denervation found in CD chronic phase as shown in human organs such as the heart (Almeida et al 1988), esophagus (Adad et al 1991), colon (Corbett et al 2001), pancreas (Dos Santos et al 2004), and skeletal muscles (Faria et al 1988). They would be related to a reflex stimulus of the salivary glands, which may cause hypersecretion of saliva.…”

Section: Discussionmentioning

confidence: 97%

Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease

et al. 2005

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The purpose of this study was to evaluate morphologically the tongue of individuals with chronic Chagas disease (CD) in comparison to the non-chagasic ones. Twenty-four protocol cases of autopsies were selected. They were subdivided into CD patients (10 cases) and non-chagasic ones (14 cases). The morphometric analysis was accomplished for the tongue muscle and salivary glands duct lumen area. In three CD patients, perineuritis was found, and two of them showed megaesophagus and megacolon. The intensity of the inflammation in the von Ebner's glands, the tongue muscles, and the salivary glands duct lumen area was significantly higher in the CD patients. We concluded that the CD patients show salivary glands duct dilatation, which probably would have a relation with alterations in the autonomic nervous system. The inflammation found in CD patients is in accordance with that described in comparative studies on the digestive tract and heart. These morphological findings suggest that the histopathological analysis of the tongue associated with other organs, or even in an isolated manner, can add in the diagnosis and pathogenesis of the CD chronic phase.

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Section: Discussionmentioning

confidence: 97%

Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease

et al. 2005

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“…Thus, in considering potential mechanisms for reduced insulin secretion in the setting of T. cruz i infection, pancreatic nerve cells have been examined (dos Santos et al 1999a; dos Santos et al 2004; Guariento et al 1994; Guariento et al 1993; Oliveira et al 1993). In fact, it was suggested that neuritis and autonomic dysfunction were etiologic factors for pancreatic disease in this setting (dos Santos et al 2004). The Rocha group (1998) compared pancreatic sections obtained from 14 individuals with chronic Chagas disease to 14 controls and found the total pancreatic neuron counts to be significantly lower in the samples from infected individuals.…”

Section: Evidence From Human Studiesmentioning

confidence: 99%

“…The dos Santos group (2004) employing a hamster model, investigated the consequence of T. cruzi infection on pancreatic β cells. Blood glucose and insulin levels in circulation were sampled in the animals during the acute phase (every 15 days until 60 dpi) as well as in the chronic phase (4 times between 105 and 375 dpi).…”

Section: Evidence From Preclinical Studies That T Cruzi Infection Rementioning

confidence: 99%

Alterations in pancreatic β cell function and Trypanosoma cruzi infection: evidence from human and animal studies

et al. 2016

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The parasite Trypanosoma cruzi causes a persistent infection, Chagas disease, affecting millions of persons in endemic areas of Latin America. As a result of immigration, this disease has now been diagnosed in non-endemic areas worldwide. Although, the heart and gastrointestinal tract are the most studied, the insulin secreting β cell of the endocrine pancreas is also a target of infection. In this review, we summarize available clinical and laboratory evidence to determine whether T. cruzi-infection-mediated changes of β cell function is likely to contribute to the development of hyperglycemia and diabetes. Our literature survey indicates that T. cruzi infection of humans and of experimental animals relates to altered secretory behavior of β cells. The mechanistic basis of these observations appears to be a change in stimulus-secretion pathway function rather than the loss of insulin producing β cells. Whether this attenuated insulin release ultimately contributes to the pathogenesis of diabetes in human Chagas disease however, remains to be determined. Since the etiologies of diabetes are multifactorial including genetic and life-style factors, the use of cell- and animal-based investigations, allowing direct manipulation of these factors, are important tools in testing if reduced insulin secretion has a causal influence on diabetes in the setting of Chagas disease. Long-term clinical investigations will be required to investigate this link in humans.

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“…We investigated the presence of amastigote nests in the whole tissue area and the inflammatory processes were semiquantitatively classified into absent (-), mild (+), moderate (++), and severe (+++), as previously described [23][24][25][26] .…”

Section: Histopathologymentioning

confidence: 99%

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

Gómez-Hernández

Rezende-Oliveira

Nascentes

et al. 2011

Rev. Soc. Bras. Med. Trop.

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Introduction: For a long time, the importance of Chagas disease in Mexico, where many regarded it as an exotic malady, was questioned. Considering the great genetic diversity among isolates of Trypanosoma cruzi, the importance of this biological characterization, and the paucity of information on the clinical and biological aspects of Chagas disease in Mexico, this study aimed to identify the molecular and biological characterization of Trypanosoma cruzi isolates from different endemic areas of this country, especially of the State of Jalisco. Methods: Eight Mexican Trypanosoma cruzi strains were biologically and genetically characterized (PCR specific for Trypanosoma cruzi, multiplex-PCR, amplification of space no transcript of the genes of the mini-exon, amplification of polymorphic regions of the mini-exon, classification by amplification of intergenic regions of the spliced leader genes, RAPD -(random amplified polymorphic DNA). Results: Two profiles of parasitaemia were observed, patent (peak parasitaemia of 4.6×10 6 to 10 7 parasites/mL) and subpatent. In addition, all isolates were able to infect 100% of the animals. The isolates mainly displayed tropism for striated (cardiac and skeletal) muscle. PCR amplification of the mini-exon gene classified the eight strains as TcI. The RAPD technique revealed intraspecies variation among isolates, distinguishing strains isolated from humans and triatomines and according to geographic origin. Conclusions: The Mexican T. cruzi strains are myotrophic and belong to group TcI. Keywords: Trypanosoma cruzi. Mexico. Biological characterization. Genetic characterization. RESUMOIntrodução: Durante muito tempo, foi questionada a importância da doença de Chagas no México onde muitos a consideravam um padecimento exótico. Considerando a grande diversidade genética existente, entre os isolados de Trypanosoma cruzi, a importância da caracterização biológica desses e o escasso número de informações sobre os aspectos clínicos e biológicos da doença de Chagas no México, o objetivo deste trabalho foi realizar a caracterização biológica e molecular de isolados de Trypanosoma cruzi originários de diferentes áreas endêmicas deste país, principalmente do Estado de Jalisco. Métodos: Oito cepas mexicanas de Trypanosoma cruzi foram caracterizadas biologicamente e geneticamente (PCR específica para Trypanosoma cruzi, PCR-multiplex, amplificação do espaço não transcrito dos genes do mini-exon, amplificação das regiões polimórficas do gene do mini-exon, classificação pela amplificação de regiões intergênicas dos genes do spliced leader, RAPD -random amplified polymorphic DNA). Resultados: Foram observados dois tipos de parasitemia: patente com picos máximos de parasitemia entre 4,6x10 6 e 10 7 parasitas/mL e subpatente. Além disso, todos os isolados foram capazes de infectar 100% dos animais. Observou-se tropismo predominante pelo músculo estriado (cardíaco e esquelético). As técnicas de PCR do gene do mini-éxon classificaram as oito cepas como TcI e a técnica de RAPD mostrou variaçã...

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Functional and histopathological study of the pancreas in hamsters (Mesocricetus auratus) infected and reinfected with Trypanosoma cruzi

Cited by 13 publications

References 31 publications

Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease

Inflammation and glandular duct dilatation of the tongue from patients with chronic Chagas disease

Alterations in pancreatic β cell function and Trypanosoma cruzi infection: evidence from human and animal studies

Molecular characterization of Trypanosoma cruzi Mexican strains and their behavior in the mouse experimental model

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