Functional and Biochemical Parameters of Erythrocytes during Mexicor Treatment in Posttraumatic Period after Experimental Blood Loss and Combined Traumatic Brain Injury

Deryugina, А. V.; Shumilova, Anastasiia; Filippenko, E. S.; Galkina, Ya. V.; Симутис, И. С.; Boyarinov, G. A.

doi:10.1007/s10517-017-3918-4

Cited by 14 publications

(11 citation statements)

References 5 publications

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“…Discussing the mechanisms of changes in the phase portrait and EPME in various pathologies before and after the treatment, it may be stated that these measurements do not depend on the etiology of the disease and are of the typical character. Based on the previously found dependence of EPME on the stress reaction development and triggering of the adaptive processes [9,10] it may be considered that emergence of echiocytic cell transformation and EPME reduction is caused by the development of stress reaction the intensity of which is manifested by a significant EPME decrease and appearance of spheroechinocytes. But in Thus, the results obtained allow us to state that during the development of a pathological process, stress reaction is progressing manifesting itself in the morphological changes of erythrocytes and their electrophoretic mobility.…”

Section: Resultsmentioning

confidence: 99%

Alterations in the Phase Portrait and Electrophoretic Mobility of Erythrocytes in Various Diseases

Deryugina¹,

Ivashchenko²,

Ignatiev³

et al. 2019

Sovrem Tehnol Med

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The aim of the investigation was to study a phase portrait, electrophoretic mobility of erythrocytes (EPME), and clinical and laboratory blood indices in patients with various types of diseases. Materials and Methods. A phase portrait, EPME, and clinical and laboratory blood indices have been studied in patients with cardiovascular, bronchopulmonary, gynecological, and gastroenterological diseases. The phase portrait was investigated using laser interferometry technique, EPME was determined by microelectrophoresis. The clinical and laboratory indices included counting the number of erythrocytes and leukocytes, measurements of hemoglobin content and ESR by means of standard clinical methods. The phase portrait and EPME were studied under the action of adrenaline and cortisol in experiments in vitro. Results. Using laser interferometry, phase images of erythrocytes in cardiovascular, gastroenterological, bronchopulmonary, and gynecological diseases have been obtained. The analysis of the gallery of erythrocyte phase images has shown that alterations were unidirectional in all types of pathologies and were characterized by the increase of echinocyte number. The morphological modification of erythrocytes correlated with EPME changes and clinical and laboratory blood indices and reflected the degree of stress and triggering of adaptive processes in the course of the conducted therapy. Influence of stress-realizing systems has been confirmed by the in vitro experiments under the action of adrenaline and cortisol. Conclusion. Indices of EPME and erythrocyte phase portrait obtained with the help of the current techniques of cellular microelectrophoresis and laser interferometry provide the opportunity to obtain new information on the internal structure of cytoobjects without special preparations of specimens and contrast or fluorescent probes. These indices may be applied for quantitative characteristic of the stress reaction degree and the development of adaptive processes which is of great importance for the transition to personified medicine.

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Section: Resultsmentioning

confidence: 99%

Alterations in the Phase Portrait and Electrophoretic Mobility of Erythrocytes in Various Diseases

Deryugina¹,

Ivashchenko²,

Ignatiev³

et al. 2019

Sovrem Tehnol Med

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“…(20) Earlier it was shown that this group of preparations decreases the intensity of LPO both in blood plasma and in cell membranes. (21) It is very important to note that mexicor and cytoflavin promoted a decrease in the LMMWS content. The catabolic component of LMMWS is represented by low molecular weight compounds-protein breakdown products.…”

Section: Discussionmentioning

confidence: 99%

Endogenous Intoxication and the Role of Antioxidants in Motion Activity Correction with Traumatic Brain Injury in Rat Model

Polozova¹,

Bojarinov²,

Ivashchenko³

et al. 2019

IJBM

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The aim of this study was to assess the effectiveness of cytoflavin and mexicor in the posttraumatic period of traumatic brain injury (TBI). Materials and Methods:The experiments were carried out on 60 white non pedigree female rats weighing 180-200 g, with 15 rats in each series. TBI was modeled by a free-falling weight drop of 100 g from a height of 80 cm on the parietal-occipital area of the head. Blood samples were taken from the sublingual vein in an amount of 2.0 ml on Days 1, 3, 7, and 12 after the alteration. After TBI, in Group 1, 15 rats received an intraperitoneal injection of 2-ethyl-6-methyl-3-hydroxypyridine succinate (mexicor) for 10 days in a daily dose of 8.0 mg/kg. In Group 2, 15 rats received an intraperitoneal injection of cytoflavin for 10 days in a daily dose of 0.2 ml/kg. The activity of lipid peroxidation and antioxidant protection system in the blood plasma was determined by a biochemicoluminescence method. Analysis of animal motion activity included the determination of the ability to balance and to stay at the wooden bar, time spent for moving on the bar from the bright light source to the darkroom, and paw slip frequency.Results: Mexicor and cytoflavin decreased the level of oxidative processes in rat model with TBI and the development of secondary brain injury. The positive dynamics in restoring pro-and antioxidant system balance was combined with positive changes in motor function.

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“…The value of potential gradient was determined using the formula: Н=I/g×χ, where I -amperage, g -chamber cross section, χelectrical conductivity of the media. (38,39) Animals were housed in keeping with the rules for good laboratory practice. Experiment was performed in accordance with the Guide for the Care and Use of Laboratory Animals (the institute of Laboratory Animal Resources, 1996) and with approval of local Ethics Committee.…”

Section: Methodsmentioning

confidence: 99%

Stress-Related Effects of Low-Intensity Laser Irradiation

Deryugina¹,

Ivashchenko²,

Ignatyev³

et al. 2019

IJBM

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The purpose of this study was to investigate the effect of low-level laser (light) therapy (LLLT) on the electrokinetic properties of red blood cells (RBCs), taking into account the activity of stress-realizing systems of the body. The RBC electrophoretic mobility (RBCEM) was used as an index of stress reaction. The experiment included two series: in vivo and in vitro. Analyzing the LLLT effect on RBCEM, we can assume that the body's response to LLLT is associated with a short-term activation of the sympathoadrenal system and the subsequent longer reaction of the hypothalamic-pituitary-adrenal axis. Through activation of the hypothalamic-pituitary-adrenal axis, LLLT can indirectly cause the development of adaptation processes in the body.

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Functional and Biochemical Parameters of Erythrocytes during Mexicor Treatment in Posttraumatic Period after Experimental Blood Loss and Combined Traumatic Brain Injury

Cited by 14 publications

References 5 publications

Alterations in the Phase Portrait and Electrophoretic Mobility of Erythrocytes in Various Diseases

Alterations in the Phase Portrait and Electrophoretic Mobility of Erythrocytes in Various Diseases

Endogenous Intoxication and the Role of Antioxidants in Motion Activity Correction with Traumatic Brain Injury in Rat Model

Stress-Related Effects of Low-Intensity Laser Irradiation

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