Functional analysis on the 5′-flanking region of carp fast skeletal myosin heavy chain genes for their expression at different temperatures

Kobiyama, Atsushi; Hirayama, Makoto; Muramatsu-Uno, Maiko; Watabe, Shugo

doi:10.1016/j.gene.2005.12.006

Cited by 15 publications

(17 citation statements)

References 34 publications

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“…Although a considerable progress has been made in elucidating the molecular genetics underlying the muscle-specific expression of MYHs in carp and medaka mostly in a temperature dependent manner (Gauvry et al, 1996;Kobiyama et al, 2006;Liang et al, 2008), little is currently known regarding the molecular mechanisms governing gene expression even within the fast subfamily at embryonic and larval stages of torafugu. We recently reported that the 2.1 kb 5′-flanking region of torafugu MYH M743-2 regulated developmental muscle-specific expression (Yasmin et al, 2011).…”

Section: Discussionmentioning

confidence: 99%

“…1). These binding sites have been reported to be involved in the transcriptional regulation of many muscle-specific genes during development in fish (Du et al, 2006;Kobiyama et al, 2006;Liang et al, 2008). To determine the role of these MyoD and MEF2 binding sites, we deleted these sites individually or in various combinations (Fig.…”

Section: Myod and Mef2 Binding Sites Are Involved In The Transcriptiomentioning

confidence: 99%

“…However, the transcriptional mechanisms in regulating the expression of MYH M743-2 are not well defined. Transcriptional regulatory regions have been mapped for a small number of skeletal muscle-specific genes in fish, identifying both unique and shared cis-acting elements (Chen et al, 2007;Du et al, 2003;Kobiyama et al, 2006;Liang et al, 2008). Therefore it is important to identify cis-acting elements located at various distances upstream or downstream of the promoter responsible for the expression of torafugu MYH M743-2 .…”

Section: Introductionmentioning

confidence: 99%

“…The previous studies revealed that MEF2 and SRF binding elements regulate fast skeletal MYH transcription in vivo (Allen et al, 2005). So far, the transcriptional activities of MYHs have been extensively investigated on the fast skeletal MYHs of mammals (Allen et al, 2005;Harrison et al, 2011;Lakich et al, 1998;Swoap, 1998) and fish (mainly carp and medaka) in a temperature dependent manner (Gauvry et al, 1996;Kobiyama et al, 2006;Liang et al, 2008). However, it is still far from comprehensive understanding of the transcription factors functioning in the regulation of embryonic and larval fast-type MYHs expression in fish.…”

Section: Introductionmentioning

confidence: 99%

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Multiple cis-elements in the 5′-flanking region of embryonic/larval fast-type of the myosin heavy chain gene of torafugu, MYHM743-2, function in the transcriptional regulation of its expression

Asaduzzaman

Kinoshita

Siddique

et al. 2011

Gene

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Section: Discussionmentioning

confidence: 99%

Section: Myod and Mef2 Binding Sites Are Involved In The Transcriptiomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Multiple cis-elements in the 5′-flanking region of embryonic/larval fast-type of the myosin heavy chain gene of torafugu, MYHM743-2, function in the transcriptional regulation of its expression

Asaduzzaman

Kinoshita

Siddique

et al. 2011

Gene

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“…To localize functional regions of MYHs responsible for its expression in a temperature-dependent manner in our previous study using common carp, a series of deletion constructs was prepared from the 5Ј-flanking region, inserted upstream from the luciferase gene in a commercially available plasmid, and injected into the dorsal fast muscle of carp acclimated to 10 and 30°C (27). Promoter activity analysis showed that Ϫ1 kbp of MYH10, the gene predominantly expressed in the 10°C-acclimated fish, contained the region responsible for a temperaturedependent gene expression.…”

Section: Discussionmentioning

confidence: 99%

Fast skeletal muscle myosin heavy chain gene cluster of medakaOryzias latipesenrolled in temperature adaptation

Liang

Kobiyama

Shimizu

et al. 2007

Physiological Genomics

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Liang C-S, Kobiyama A, Shimizu A, Sasaki T, Asakawa S, Shimizu N, Watabe S. Fast skeletal muscle myosin heavy chain gene cluster of medaka Oryzias latipes enrolled in temperature adaptation. Physiol Genomics 29: [201][202][203][204][205][206][207][208][209][210][211][212][213][214] 2007. First published January 16, 2007; doi:10.1152/physiolgenomics.00078.2006.-To disclose mechanisms involved in temperature acclimation of fish muscle, we subjected eurythermal fish of medaka Oryzias latipes to cloning of myosin heavy chain genes (MYHs). We cloned cDNAs encoding fast skeletal muscle myosin heavy chain (MYH) isoforms from cDNA libraries of medaka acclimated to 10 and 30°C and observed that different MYH cDNA clones are expressed in the two temperatureacclimated fish. Subsequently, we isolated several overlapping MYH contigs by shotgun cloning strategy from a medaka genomic library. Contig assembly of the complete medaka MYH (mMYH) locus of 219 kbp revealed a cluster of tandemly arrayed 11 mMYHs, in which eight genes are actually transcribed, with the remaining three being pseudogenes. Expression analysis of the transcribed genes revealed that two genes were each highly expressed in medaka acclimated to 10 and 30°C, whereas comparatively lower expression levels of the three genes were exclusively observed in medaka acclimated to 30°C. cDNAs of the remaining genes were too underrepresented in the libraries to determine the expression levels, and the transcripts could only be obtained by reverse transcription-polymerase chain reaction. Deduced amino acid sequences in the loop 1 and loop 2 regions of mMYHs were highly variable, suggesting that these isoforms were functionally different. The present findings consolidate our knowledge on teleost MYH multigene family and would provide further insight into the mechanisms by which expressions of individual MYH molecules are fine-tuned with environmental temperature fluctuations with further functional analysis of the genes concerned. cDNA library; genomic bacterial artificial chromosome library; temperature acclimation; myofibrillar Mg 2ϩ -ATPase activity MYOSINS ARE ACTIN-BASED MOLECULAR motors that convert chemical energy released from the hydrolysis of ATP into mechanical force in eukaryotic cells. Numerous myosins comprising 18 classes (48, 52) have been described, including a subgroup of class II that contains sarcomeric myosins functionally to drive muscle contraction. Class II or conventional two-headed myosin is a heterohexamer consisting of two heavy chains (MYHs) of ϳ200 kDa and four light chains of ϳ20 kDa. The NH 2 -terminal half of each MYH folds into a globular head, termed subfragment-1 (S1), which contains an ATP-and an actin-binding site (30). The COOH-terminal halves of two heavy chains form an ␣-helical coiled coil structure, a rod. Rods can be separated further into two fragments by limited proteolysis; an NH 2 -terminal half called subfragment-2 (S2) and a COOH-terminal half of light meromyosin (LMM) (11). Furthermore, tryptic digestion of S1 results in...

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Early development of medaka Oryzias latipes muscles as revealed by transgenic approaches using embryonic and larval types of myosin heavy chain genes

Ono

Kinoshita

Ikeda

et al. 2010

Developmental Dynamics

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We cloned three full-length cDNAs encoding myosin heavy chains (MYHs) previously found to be expressed in embryos or larvae of medaka Oryzias latipes. Based on cDNA sequence information, the three medaka MYH genes, mMYH emb1 , mMYH L1 and mMYH L2 , were localized on the chromosomes. In vivo promoter assay using the gene encoding green or red fluorescent protein and linked to the 5 0 -flanking region of mMYH demonstrated that the transcripts of fast-type mMYH emb1 , first expressed in embryos but belonging to the adult type in phylogenetic analysis, were located in the horizontal myoseptum. On the other hand, embryonic fast-type mMYH L1 and mMYH L2 were expressed in the whole myotomes. Interestingly, cells expressing mMYH emb1 were localized together with engrailed, and cyclopamine, which blocks hedgehog signaling, inhibited mMYH emb1 expression as well as the formation of the horizontal myoseptum, suggesting that muscle pioneer cells express mMYH emb1 as a key protein in the formation of the horizontal myoseptum.

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Functional analysis on the 5′-flanking region of carp fast skeletal myosin heavy chain genes for their expression at different temperatures

Cited by 15 publications

References 34 publications

Multiple cis-elements in the 5′-flanking region of embryonic/larval fast-type of the myosin heavy chain gene of torafugu, MYHM743-2, function in the transcriptional regulation of its expression

Multiple cis-elements in the 5′-flanking region of embryonic/larval fast-type of the myosin heavy chain gene of torafugu, MYHM743-2, function in the transcriptional regulation of its expression

Fast skeletal muscle myosin heavy chain gene cluster of medakaOryzias latipesenrolled in temperature adaptation

Early development of medaka Oryzias latipes muscles as revealed by transgenic approaches using embryonic and larval types of myosin heavy chain genes

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